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ProteomicsSpic a specific marker of ground state pluripotency that regulates one carbon metabolism
Understanding mechanisms of epigenetic regulation in embryonic stem cells (ESCs) is of fundamental importance for stem cell and developmental biology. Here we identify Spic, a member of the ETS family of transcription factors, as a specific marker of ground state pluripotency. We show that Spic is r...
ORGANISM(S): Mus musculus (Mouse) 
2025-05-06 | PXD039213 | Pride
Spic Sam Embryonic stem 2il One carbone metabolism Epigenetic Ground state Bhmt 
ProteomicsPramel7-Cul2 axis establishes ground-state gene expression in embryonic stem cells
Pluripotency is established in E4.5 preimplantation epiblast, the founder populations of the adult embryo. The establishment of in vitro models that closely resemble ground-state pluripotency represents an important opportunity to study early embryo development. Pramel7, a protein highly expressed i...
ORGANISM(S): Mus musculus (Mouse) 
2024-01-08 | PXD032184 | Pride
Pluripotency Embryonic stem cells Pramel7 Proteasome Cullin 2 
GenomicsChIP-seq of Klf2 in mouse embryonic stem cells (Serum/LIF and 2i)
The self-renewing pluripotent state was first captured in mouse embryonic stem cells (mESCs) over two decades ago. The standard condition requires the presence of serum and LIF, which provide growth promoting signals for cell expansion. However, there are pro-differentiation signals which destabiliz...
ORGANISM(S): Mus musculus 
2014-06-04 | E-MTAB-2365 | biostudies-arrayexpress
TranscriptomicsRNA-seq analysis of ES cell populations with different functional potentials in serum/LIF and 2i/LIF, as well as analysis of the transcriptional effect of LIF in ground state (2i) culture conditions.
We report that ES cells cultured in ground state (2i and 2i/LIF) culture conditions are heterogeneous and show heterogeneus expression of extraembryonic markers. Using a highly sensitive reporter for the endoderm marker Hex we can sort Hex high and low populations from either serum/LIF or 2i/LIF and...
ORGANISM(S): Mus musculus 
2013-06-11 | E-GEOD-45182 | biostudies-arrayexpress
GenomicsDerivation of novel human ground state naM-CM-/ve pluripotent stem cells
Mouse embryonic stem (ES) cells are isolated from the inner cell mass of blastocysts, and can be preserved in vitro in a naive inner-cell-mass-like configuration by providing exogenous stimulation with leukaemia inhibitory factor (LIF) and small molecule inhibition of ERK1/ERK2 and GSK3b signalling ...
ORGANISM(S): Homo sapiens 
2013-11-28 | E-GEOD-52617 | biostudies-arrayexpress
ProteomicsMPP8 is essential for sustaining self-renewal of ground-state pluripotent stem cells
Deciphering the mechanisms that control the pluripotent ground state is key for understanding embryonic development. Nonetheless, the epigenetic regulation of ground-state mouse embryonic stem cells (mESCs) is not fully understood. Here, we identify the epigenetic protein MPP8 as being essential for...
ORGANISM(S): Mus musculus (Mouse) 
2021-03-21 | PXD019345 | Pride
Lc-ms/ms Ip-ms Embryonic stem cells 
TranscriptomicsTransition of embryonic stem cells from ground state pluripotency
Mouse embryonic stem cells were maintained in minimal and chemically-defined culture conditions supporting naive pluripotency. Inhibitors of the Gsk3 (CHIR99021) and Mek/Erk (PD0325901) pathways were withdrawn, cultures maintained for 24 hours, and subsequently sorted by flow cytometry based on fluo...
ORGANISM(S): Mus musculus 
2017-02-22 | E-MTAB-5304 | biostudies-arrayexpress
ProteomicsThe translational landscape of naïve and primed state pluripotency
Translational control plays a central role in regulation of gene expression and can lead to significant divergence between mRNA- and protein-abundance. The translational landscape of early mammalian development and its impact on cellular proteome, however, remains largely un-explored. Here we used g...
ORGANISM(S): Mus musculus (Mouse) 
2020-04-03 | PXD014528 | Pride
Ribosome profiling Proteome Translation Embryonic stem cells Ground state pluripotency 
ProteomicsQuantitative subcellular proteomics using SILAC reveals enhanced metabolic buffering in ground state pluripotency
The ground state of pluripotency is defined as a minimal unrestricted state as present in the Inner Cell Mass (ICM). Mouse embryonic stem cells (ESCs) grown in a defined serum-free medium with two kinase inhibitors (‘2i’) reflect this state, whereas ESCs grown in the presence of serum (‘serum’) shar...
ORGANISM(S): Mus musculus (Mouse) 
2018-11-07 | PXD009217 | Pride
Embryonic stem cells Naïve pluripotency Metabolism Silac 
TranscriptomicsTransition of embryonic stem cells from ground state pluripotency (RNA-seq)
Mouse embryonic stem cells were maintained in minimal and chemically-defined culture conditions supporting naive pluripotency. Inhibitors of the Gsk3 (CHIR99021) and Mek/Erk (PD0325901) pathways were withdrawn, cultures maintained for 24 hours, and subsequently sorted by flow cytometry based on fluo...
ORGANISM(S): Mus musculus 
2017-02-22 | E-MTAB-5305 | biostudies-arrayexpress
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