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ProteomicsSpic a specific marker of ground state pluripotency that regulates one carbon metabolism
Understanding mechanisms of epigenetic regulation in embryonic stem cells (ESCs) is of fundamental importance for stem cell and developmental biology. Here we identify Spic, a member of the ETS family of transcription factors, as a specific marker of ground state pluripotency. We show that Spic is r...
ORGANISM(S): Mus musculus (Mouse) 
2025-05-06 | PXD039213 | Pride
Spic Sam Embryonic stem 2il One carbone metabolism Epigenetic Ground state Bhmt 
ProteomicsThe translational landscape of naïve and primed state pluripotency
Translational control plays a central role in regulation of gene expression and can lead to significant divergence between mRNA- and protein-abundance. The translational landscape of early mammalian development and its impact on cellular proteome, however, remains largely un-explored. Here we used g...
ORGANISM(S): Mus musculus (Mouse) 
2020-04-03 | PXD014528 | Pride
Ribosome profiling Proteome Translation Embryonic stem cells Ground state pluripotency 
TranscriptomicsTransition of embryonic stem cells from ground state pluripotency
Mouse embryonic stem cells were maintained in minimal and chemically-defined culture conditions supporting naive pluripotency. Inhibitors of the Gsk3 (CHIR99021) and Mek/Erk (PD0325901) pathways were withdrawn, cultures maintained for 24 hours, and subsequently sorted by flow cytometry based on fluo...
ORGANISM(S): Mus musculus 
2017-02-22 | E-MTAB-5304 | biostudies-arrayexpress
TranscriptomicsTransition of embryonic stem cells from ground state pluripotency (RNA-seq)
Mouse embryonic stem cells were maintained in minimal and chemically-defined culture conditions supporting naive pluripotency. Inhibitors of the Gsk3 (CHIR99021) and Mek/Erk (PD0325901) pathways were withdrawn, cultures maintained for 24 hours, and subsequently sorted by flow cytometry based on fluo...
ORGANISM(S): Mus musculus 
2017-02-22 | E-MTAB-5305 | biostudies-arrayexpress
ProteomicsPramel7-Cul2 axis establishes ground-state gene expression in embryonic stem cells
Pluripotency is established in E4.5 preimplantation epiblast, the founder populations of the adult embryo. The establishment of in vitro models that closely resemble ground-state pluripotency represents an important opportunity to study early embryo development. Pramel7, a protein highly expressed i...
ORGANISM(S): Mus musculus (Mouse) 
2024-01-08 | PXD032184 | Pride
Pluripotency Embryonic stem cells Pramel7 Proteasome Cullin 2 
ProteomicsTracking the embryonic stem cell transition from ground state pluripotency
Mouse embryonic stem (ES) cells are locked into self-renewal by shielding from inductive cues. Release from this ground state in minimal conditions offers a system for delineating developmental progression from naive pluripotency. Here we examined the initial transition process. The ES cell populati...
ORGANISM(S): Mus musculus (Mouse) 
2017-02-22 | PXD005581 | Pride
Es cell Pluripotency Epiblast Rex1 
ProteomicsQuantitative subcellular proteomics using SILAC reveals enhanced metabolic buffering in ground state pluripotency
The ground state of pluripotency is defined as a minimal unrestricted state as present in the Inner Cell Mass (ICM). Mouse embryonic stem cells (ESCs) grown in a defined serum-free medium with two kinase inhibitors (‘2i’) reflect this state, whereas ESCs grown in the presence of serum (‘serum’) shar...
ORGANISM(S): Mus musculus (Mouse) 
2018-11-07 | PXD009217 | Pride
Embryonic stem cells Naïve pluripotency Metabolism Silac 
GenomicsChIP-seq of Klf2 in mouse embryonic stem cells (Serum/LIF and 2i)
The self-renewing pluripotent state was first captured in mouse embryonic stem cells (mESCs) over two decades ago. The standard condition requires the presence of serum and LIF, which provide growth promoting signals for cell expansion. However, there are pro-differentiation signals which destabiliz...
ORGANISM(S): Mus musculus 
2014-06-04 | E-MTAB-2365 | biostudies-arrayexpress
TranscriptomicsJun-mediated changes in cell adhesion contribute to embryonic stem cell exit from ground state pluripotency
Embryonic stem cells (ESC) are able to give rise to any somatic cell type. A lot is known about how ESC pluripotency is maintained, but comparatively less is known about how differentiation is promoted. Cell fate decisions are regulated by interactions between signalling and transcriptional networks...
ORGANISM(S): Mus musculus 
2015-12-07 | E-MTAB-4068 | biostudies-arrayexpress
GenomicsPRAMEL7 and CUL2 decrease NuRD stability to establish ground-state pluripotency
PRAMEL7 and CUL2 decrease NuRD stability to establish ground-state pluripotency
PRJNA889810 | ENA
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