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High-molecular-weight glutenin subunits (HMW-GS) play an essential role in the end-use quality of wheat (Triticum aestivum L.). We developed a targeted liquid chromatography-tandem mass spectrometry method in parallel reaction monitoring (LC-MS/MS-PRM) to detect and differentiate wheat HMW-GS in Ger...
ORGANISM(S): Triticum aestivum (Wheat) 
2026-09-10 | PXD058934 | Pride
PII proteins are pivotal regulators of nitrogen metabolism in most prokaryotes, controlling the activities of many targets, including nitrogen assimilation enzymes, two component regulatory systems and ammonium transport proteins. Escherichia coli contains two PII-like proteins, PII (product of glnB...
ORGANISM(S): Mycobacterium tuberculosis 
We investigated design principles of DARPin-based bioPROTACs targeting the model substrate GS-eGFP (eGFP with an n-terminal tag, necessary for proteasomal unfolding). Using a highly active E3 ligase domain from CHIP and nine well-characterized DARPins, we evaluated their ability to induce proteasoma...
ORGANISM(S): Homo sapiens (Human) 
2025-06-05 | PXD063877 | Pride
We performed unbiased quantitative mass spectrometry (MS)-based analysis to measure changes in protein abundance and oxidation in cerebrospinal fluid (CSF) from a cohort of Amyotrophic Lateral Sclerosis (ALS) patients and healthy controls at two time points (approximately four months apart) to captu...
ORGANISM(S): Homo sapiens (Human) 
2025-07-14 | PXD053913 | Pride
High-molecular-weight glutenin subunits (HMW-GS) play an essential role in the end-use quality of wheat (Triticum aestivum L.). We developed a targeted liquid chromatography-tandem mass spectrometry method in parallel reaction monitoring (LC-MS/MS-PRM) to detect and differentiate wheat HMW-GS in Ger...
ORGANISM(S): Triticum Aestivum 
2026-03-05 | PXD062483 | panorama
Wheat gluten proteins are key determinants of baking quality. While untargeted proteomics enable relative quantification, absolute quantities of specific gluten protein groups are not available so far. We developed a targeted liquid chromatography with tandem mass spectrometry (LC-MS/MS) stable isot...
ORGANISM(S): Triticum Aestivum 
2026-03-05 | PXD072549 | panorama
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