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ProteomicsCSF proteome mapping: Human cerebrospinal fluid LC-MS/MS, Part 2
3 mL CSF from a pool of 21 neurologically healthy donors was separated into a bound and a depleted fraction using a MARS Hu-14 column. This experiment represents the depleted fraction. Gel separation on a gradient gel (SDS-PAGE) followed and the gel lane was cut into 46 fractions. Each fraction was ...
ORGANISM(S): Homo sapiens (Human) 
2014-08-04 | PXD000652 | Pride
Homo sapiens Cerebrospinal fluid Lc-ms/ms 
ProteomicsCSF proteome mapping: Human cerebrospinal fluid LC-MS/MS, Part 1
3 mL CSF from a pool of 21 neurologically healthy donors was separated into a bound and a depleted fraction using a MARS Hu-14 column. This experiment represents the bound fraction. Gel separation on a gradient gel (SDS-PAGE) followed and the gel lane was cut into 37 fractions. Each fraction was in-...
ORGANISM(S): Homo sapiens (Human) 
2014-08-04 | PXD000651 | Pride
Homo sapiens Cerebrospinal fluid Lc-ms/ms 
ProteomicsCSF proteome mapping: Human plasma LC-MS/MS, Part 7
40 µL plasma from a pool of 20 neurologically healthy donors was separated into a bound and a depleted fraction using a MARS Hu-14 column. This experiment represents the depleted fraction. Sample was in-solution trypsin digested and peptides were fractionated into 67 fractions by mixed-mode reversed...
ORGANISM(S): Homo sapiens (Human) 
2014-08-04 | PXD000657 | Pride
Plasma Homo sapiens Lc-ms/ms 
ProteomicsCSF proteome mapping: Human cerebrospinal fluid LC-MS/MS, Part 4
1.6 mL CSF from a pool of 21 neurologically healthy donors was separated into a bound and a depleted fraction using a MARS Hu-14 column. This experiment represents the depleted fraction. Sample was in-solution trypsin digested and resulting peptides were fractionated into 66 fractions by mixed-mode ...
ORGANISM(S): Homo sapiens (Human) 
2014-08-04 | PXD000654 | Pride
Homo sapiens Cerebrospinal fluid Lc-ms/ms 
ProteomicsCSF proteome mapping: Human Plasma LC-MS/MS, Part 6
40 µL plasma from a pool of 20 neurologically healthy donors was separated into a bound and a depleted fraction using a MARS Hu-14 column. This experiment represents the bound fraction. Sample was in-solution trypsin digested and peptides were fractionated into 10 fractions by mixed-mode reversed ph...
ORGANISM(S): Homo sapiens (Human) 
2014-08-04 | PXD000656 | Pride
Plasma Homo sapiens Lc-ms/ms 
ProteomicsCSF proteome mapping: Human cerebrospinal fluid LC-MS/MS, Part 5
1.6 mL CSF from a pool of 21 neurologically healthy donors was separated into a bound and a depleted fraction using a MARS Hu-14 column. This experiment represents the bound fraction. Sample was in-solution trypsin digested and peptides were fractionated into 10 fractions by mixed-mode reversed phas...
ORGANISM(S): Homo sapiens (Human) 
2014-08-04 | PXD000655 | Pride
Cerebrospinal fluid. homo sapiens Lc-ms/ms 
ProteomicsCSF proteome mapping: Human cerebrospinal fluid LC-MS/MS, Part 3
3 mL CSF from a pool of 21 neurologically healthy donors was in-solution trypsin digested followed by Solid-phase enrichment of N-linked glycopeptides (SPEG) using hydrazide modified beads. Glycopeptides were further fractionated into 20 fractions by mixed-mode reversed phase anion exchange (MM(RP-A...
ORGANISM(S): Homo sapiens (Human) 
2014-08-04 | PXD000653 | Pride
Homo sapiens Cerebrospinal fluid Lc-ms/ms 
ProteomicsCerebrospinal fluid 20 μg protein digest mixed-mode fractionation 11 fractions
A CSF fractionation experiment was conducted, where trypsin digested 20 µg un-depleted CSF peptides were separated into eleven fractions and analysed using an QExactive HF, resulting in 1194 identified proteins.
ORGANISM(S): Homo sapiens (Human) 
2020-09-25 | PXD013449 | Pride
Cerebrospinal fluid Human 
ProteomicsMultiple sclerosis biomarker discovery in cerebrospinal fluid using glycopeptide enrichment, TMT labeling and LC-MS/MS
Multiple sclerosis biomarker discovery in pooled cerebrospinal fluid (CSF) using glycopeptide enrichment, TMT labeling and LC-MS/MS. Comparing 3 pools of CSF from relapsing-remitting MS (RRMS) patients to 3 pools of CSF from patients with other neurological diseases (OND). To reveal protein groups a...
ORGANISM(S): Homo sapiens (Human) 
2016-10-13 | PXD004540 | Pride
Human Cerebrospinal fluid Tmt Discovery Glycopeptide enrichment Lc-ms/ms 
ProteomicsParallel reaction monitoring assay development for multiple sclerosis biomarkers in cerebrospinal fluid.
We have developed and tested parallel reaction monitoring (PRM) assays for 25 proteins highly relevant as cerebrospinal fluid (CSF) biomarkers for multiple sclerosis, representing various biological processes affected in the disease. The proteins were represented by 72 peptides selected according to...
ORGANISM(S): Homo Sapiens Rattus Norvegicus 
2021-05-20 | PXD017281 | panorama
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