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ProteomicsA H2O2-Free Proximity Proteomics Technology for Exploring Dynamic Protein Complexes in Living Systems
Dynamic protein complex assembly is critical for regulating various biological processes. Proximity labeling(PL), best represented by the ascorbate peroxidase APEX2, allows these molecular events to be captured in living cells in a spatiotemporal manner. However, the hydrogen peroxide (H2O2) depende...
ORGANISM(S): Homo sapiens (Human) 
2026-04-14 | PXD064195 | Pride
Ascorbate peroxidase Tyrosine radical H2o2-free proximity labeling 
ProteomicsDefining proximity proteomics of Histone modifications by antibody-mediated protein A-APEX2 labeling
Proximity labeling catalyzed by promiscuous enzymes, such as APEX2, has emerged as a powerful approach to characterize multiprotein complexes and protein–protein interactions. However, current methods depend on the expression of exogenous fusion proteins and cannot be applied to identify proteins su...
ORGANISM(S): Mus musculus (Mouse) 
2023-07-12 | PXD028063 | Pride
Proximity labeling  post-translationally  amapex  modified histones 
ProteomicsCharacterization of microbe-containing phagosomes using APEX2-based proximity labeling
Phagocytosis is the process by which myeloid phagocytes bind and internalize potentially dangerous microbes. During phagocytosis, innate immune receptors and the associated signaling adapters are localized to the maturing phagosome compartment. We used proximity labeling of phagosomal contents (Phag...
ORGANISM(S): Candida albicans (Yeast) Mus musculus (Mouse) Escherichia coli 
2024-04-22 | PXD043088 | Pride
Phagocytosis Apex2 Pd-l1 Proximity labeling 
ProteomicsProximity labelling and SILAC based proteomic approach identifies proteins at the interface of homotypic and heterotypic cancer cell interactions.
Cell-cell interactions are critical for the growth of organisms and maintaining homeostasis. In the tumor microenvironment, these interactions promote cancer progression. Given their importance in healthy and diseased conditions, we have developed a method to analyze the cell-to-cell interactome. Ou...
ORGANISM(S): Homo sapiens (Human) 
2025-05-26 | PXD052917 | Pride
Silac based  proteomics Nano lc-ms/ms orbitap Cell-cell interactions 
ProteomicsProximity labelling and SILAC based proteomic approach identifies proteins at the interface of homotypic and heterotypic cancer cell interactions.
Cell-cell interactions are critical for the growth of organisms and maintaining homeostasis. In the tumor microenvironment, these interactions promote cancer progression. Given their importance in healthy and diseased conditions, we have developed a method to analyze the cell-to-cell interactome. Ou...
ORGANISM(S): Homo sapiens (Human) 
2025-05-26 | PXD052746 | Pride
Silac based Orbitrap Hrp Cell-cell interactions 
ProteomicsProximity labelling and SILAC based proteomic approach identifies proteins at the interface of homotypic and heterotypic cancer cell interactions.
Cell-cell interactions are critical for the growth of organisms and maintaining homeostasis. In the tumor microenvironment, these interactions promote cancer progression. Given their importance in healthy and diseased conditions, we have developed a method to analyze the cell-to-cell interactome. Ou...
ORGANISM(S): Homo sapiens (Human) 
2025-05-26 | PXD060134 | Pride
Silac based  proteomics Nano lc-ms/ms orbitap Cell-cell interactions 
ProteomicsSpatiotemporal proteomics reveals the biosynthetic lysosomal membrane protein interactome in neurons
Spatiotemporal proteomics reveals the biosynthetic lysosomal membrane protein interactome in neurons. Lysosomes are membrane-bound organelles critical for maintaining cellular homeostasis. Delivery of biosynthetic lysosomal proteins to lysosomes is crucial to orchestrate proper lysosomal function. H...
ORGANISM(S): Rattus norvegicus (Rat) 
2025-02-27 | PXD056787 | Pride
Protein synchronization Potatomap Proximity labeling 
ProteomicsAPEX2 proximity labelling of FRS2 interactors in UW228 cells
The scaffold protein FRS2 is overexpressed in medulloblastoma (MB) and correlates with aggressive tumor phenotype and poor prognosis. Studies regarding FRS2's interactome have been limited to the assembly of the well described canonical FGFR signalosome. Here, we explored the proteomic proximity of ...
ORGANISM(S): Homo sapiens (Human) 
2026-09-07 | PXD062524 | Pride
Human Medulloblastoma Interactors Apex2 Frs2 
ProteomicsUBE2QL1 prximity proteome in HeLa cells in response to lysosomal damage
SILAC-based proximity proteomics of UAPEX2-tagged UBE2QL1 in differential LLOMe-treated HeLa cells
ORGANISM(S): Homo sapiens (Human) 
2019-08-22 | PXD014521 | Pride
Lysosome repair Ube2ql1 Lysophagy 
ProteomicsLipid droplet-targeted APEX proteome
Lipid droplet (LD) function is regulated by a complement of integral and peripheral proteins that associate with the LD phospholipid monolayer. Defining the composition of the LD proteome has remained a challenge due to the presence of contaminating proteins in LD-enriched buoyant fractions. To over...
ORGANISM(S): Homo sapiens (Human) 
2017-12-22 | PXD007695 | Pride
Lipid droplet Apex Erad 
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