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ProteomicsLight-activatable, 2,5-disubstituted tetrazoles for the proteome-wide profiling of aspartates and glutamates in living bacteria
Covalent inhibitors have recently seen a resurgence of interest in drug development. Nevertheless, compounds, that do not rely on an enzymatic activity, have almost exclusively been developed to target cysteines. Expanding the scope to other amino acids would be largely facilitated by the ability to...
ORGANISM(S): Escherichia coli Staphylococcus aureus 
2020-04-14 | PXD016659 | Pride
Covalent inhibitors Antibiotics Chemoproteomics Isodtb-abpp 
ProteomicsIsotopically Labeled Desthiobiotin Azide (isoDTB) Tags Enable Global Profiling of the Bacterial Cysteinome
In the presented datasets, we use newly developed isotopically labeled desthiobiotin azide (isoDTB) tags to residue-specifically quantify cysteines in a variety of bacterial strains and a human cell line with consistently high coverage. We e.g. quantify 59% of all cysteines in essential proteins in ...
ORGANISM(S): Staphylococcus aureus 
2019-12-02 | PXD015457 | Pride
Covalent inhibitors Antibiotics Protein modifications Isotopic labeling Proteomics 
ProteomicsCompetitive Profiling of Ligandable Cysteines in Staphylococcus aureus with an Organogold Compound
With the idea of exploiting metal-templated reactions to achieve selective modification of cysteines in proteins for antibacterial applications, an organometallic cyclometalated Au(III) compound was explored in a competitive chemoproteomic approach based on the isoDTB-ABPP (isotopically labelled des...
ORGANISM(S): Staphylococcus aureus 
2022-04-19 | PXD031708 | Pride
Covalent inhibitors Organogold Competitive residue-specific proteomics Antibiotics 
ProteomicsProteome-wide profiling of the selectivity of diverse electrophiles
Targeted covalent inhibitors (TCIs) are established as powerful entities in drug discovery, but their application has so far mainly been limited to addressing cysteine. Competitive, residue-specific proteomics has been instrumental to streamline covalent drug discovery targeting cysteine. Several pr...
ORGANISM(S): Homo sapiens (Human) Staphylococcus aureus 
2025-08-09 | PXD024454 | Pride
Covalent inhibitors Chemical proteomics Activity-based protein profiling Isodtb-abpp 
ProteomicsBioresponsive pseudoGlucosinolates (psGSLs) release Isothiocyanates (ITCs) in the Presence of Nitroreductases
Glucosinolates (GSLs) are secondary metabolites produced as part of an herbivore defence system in plants of the order Brassicales. GSLs release isothiocyanates (ITCs) upon activation by the myrosinase. Beyond their herbivore feeding deterrent properties, these ITCs have multiple interesting bioacti...
ORGANISM(S): Staphylococcus aureus 
2026-09-07 | PXD049355 | Pride
Isothiocyanates Artificial glucosinolates Bioresponsive protein labelling Pseudoglucosinolates Prodrug 
ProteomicsProteome-wide profiling of the selectivity of diverse electrophiles (part 2)
Targeted covalent inhibitors (TCIs) are established as powerful entities in drug discovery, but their application has so far mainly been limited to addressing cysteine. Competitive, residue-specific proteomics has been instrumental to streamline covalent drug discovery targeting cysteine. Several pr...
ORGANISM(S): Homo sapiens (Human) Staphylococcus aureus 
2025-08-04 | PXD065811 | Pride
Human Chemical proteomics S. aureus 
ProteomicsDegrasyn exhibits antibiotic activity against multi-resistant Staphylococcus aureus by modifying several essential cysteines
In this dataset, we investigate the targets of degrasyn in the methicillin-sensitive S. aureus strain NCTC 8325. This includes data on enrichment studies and competition studies with conventional ABPP using a degrasyn-derived probe, competition studies with residue-specific proteomics using the isoD...
ORGANISM(S): Staphylococcus aureus 
2020-04-14 | PXD016413 | Pride
Chemoproteomics Antibioitcs S. aureus Isodtb-abpp 
ProteomicsRepurposing human kinase inhibitors to create an antibiotic active against drug-resistant Staphylococcus aureus
To combat methicillin-resistant Staphylococcus aureus (MRSA) infections novel drugs are desperately needed. From a screen, we found that the anti-cancer drug sorafenib effectively kills MRSA strains. By synthetic variation of key structural features, we identified a potent analog, PK150, exhibiting ...
ORGANISM(S): Staphylococcus aureus 
2019-09-09 | PXD012946 | Pride
Full proteome Affinity-based protein profiling (afbpp) Q exactive plus Chemical proteomics Staphylococcus aureus subsp. aureus nctc 8325 Secretome Surfaceome Orbitrap fusion 
ProteomicsA tailored phosphoaspartate probe unravels CprR as a response regulator in Pseudomonas aeruginosa interkingdom signaling
Pseudomonas aeruginosa is a difficult-to-treat Gram-negative bacterial pathogen causing life-threatening infections. Adaptive resistance (AR) to cationic peptide antibiotics such as polymyxin B impairs the therapeutic success. This self-protection is mediated by two component systems consisting of a...
ORGANISM(S): Pseudomonas Aeruginosa Pao1 
2021-08-19 | PXD022470 | panorama
ProteomicsXanthocillin X Effectively Kills A.Baumannii via Dysregulation of Heme Biosynthesis
Isonitrile natural products exhibit promising antibacterial activities, however, their mode of action (MoA) remains largely unknown. Based on the nanomolar potency of xanthocillin X (Xan) against diverse difficult-to-treat Gram-negative bacteria, including the critical priority pathogen Acinetobacte...
ORGANISM(S): Acinetobacter baumannii ATCC 19606 = CIP 70.34 = JCM 6841 Escherichia coli 
2021-09-09 | PXD021400 | Pride
Isonitriles Antibiotics 
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