Sort   by:  
 Page size 
ProteomicsDMSO enhances electrospray response boosting sensitivity of proteomic experiments
We report that low percentages of dimethylsulfoxide (DMSO) in liquid chromatography solvents lead to a strong enhancement of electrospray ionization of peptides, improving the sensitivity of protein identification in bottom up proteomics by up to tenfold. The method can be easily implemented on any ...
ORGANISM(S): Homo sapiens (Human) 
2013-08-07 | PXD000254 | Pride
Proteomics Liquid chromatography-tandem mass spectrometry Electrospray ionization Dimethyl sulfoxide 
ProteomicsProteome-wide purification of O-GlcNAc proteins
Global O-GlcNAc profiling: proteome-wide purification and identification of O-GlcNAc proteins using GlcNAz metabolic labeling in combination with Click chemistry and label-free LC-MS/MS-based quantification. OGA inhibition: same workflow as 'Global O-GlcNAc profiling'; inhibition of O-GlcNAcase by a...
ORGANISM(S): Homo sapiens (Human) 
2013-01-10 | PXD000061 | Pride
Proteomics Metabolic labeling O-glcnac Click chemistry 
ProteomicsMolecular characterization of HUVEC in vivo and in vitro
Proteomic and transcriptomic analysis of freshly isolated HUVEC and primary HUVEC on conventional plastic as well as bacterial-cellulose based support (Xellulin).
ORGANISM(S): Homo sapiens (Human) 
2017-06-26 | PXD003975 | Pride
Proteomics Huvec Transcriptomics 
ProteomicsCombined proteomics and miRNomics of a glioblastoma immunotherapy cohort reveals resistance factor candidates and novel counterstrategy concepts
Glioblastoma is the most prevalent and aggressive form of brain cancer. With a median overall survival of ~20 months under current standard therapy (surgery, chemotherapy, radiotherapy), novel treatment approaches are desperately needed. Immunotherapies are being investigated in clinical trials but ...
ORGANISM(S): Homo sapiens (Human) 
2020-01-27 | PXD012616 | Pride
Proteomics Immune oncology Glioblastoma Clinical cohort 
TranscriptomicsTranscriptome analysis of Bacillus subtilis salt stress adaptation
Whole-genome microarray technology and state-of-the-art proteomic techniques were applied to provide a global and time-resolved picture of the physiological response of B. subtilis cells exposed to a severe and sudden osmotic up-shift. This combined experimental approach provided quantitative data f...
ORGANISM(S): Bacillus subtilis 
2010-05-19 | E-GEOD-18345 | biostudies-arrayexpress
ProteomicsAnnotation of the domestic pig genome by quantitative proteomics
Proteogenomics analysis of porcine embryonal stages and juvenile organs.
ORGANISM(S): Sus scrofa domesticus (domestic pig) 
2017-06-21 | PXD003204 | Pride
Pig genome Proteogenomics Quantitative proteomics 
ProteomicsComparative proteomic and transcriptomic analysis of the impact of androgen stimulation and darolutamide inhibition on prostate cancer cells
Androgen receptor (AR) signaling is a major driver and therapy target in prostate cancer. Several inhibitors of AR function are approved for different stages of the disease and their impact on downstream gene transcription has been described. However, the ensuing effects of androgen and anti-androge...
ORGANISM(S): Homo sapiens (Human) 
2023-02-17 | PXD036962 | Pride
Andrigen receptor signalling Vcap Darolutamide 
ProteomicsIon mobility tandem mass spectrometry enhances performance of bottom-up proteomics
We describe a novel method utilizing ion mobility-based concentration of peptide fragment ions on a qTOF mass spectrometer improving the sensitivity of bottom-up proteomics by up to 10-fold. This enabled the identification of 7,500 human proteins within one day and 8,600 phosphorylation sites within...
ORGANISM(S): Homo sapiens (Human) 
2015-08-18 | PXD000863 | Pride
Quadrupole time of flight mass spectrometer Proteomics Ion mobility mass spectrometry Chemical proteomics Phosphoproteomics 
ProteomicsA Novel Two-Stage Tandem Mass Spectrometry Approach and Scoring Scheme for the Identification of O-GlcNAc Modified Peptides
The modification of serine and threonine residues in proteins by a single N-acetylglucosamine (O-GlcNAc) residue is an emerging post-translational modification (PTM) with broad biological implications. However, the systematic or large-scale analysis of this PTM is hampered by several factors, includ...
ORGANISM(S): Homo sapiens (Human) 
2018-06-18 | PXD010152 | Pride
O-glcnac 
ProteomicsMass spectrometry based draft of the human proteome
This PXD project contains two projects published on ProteomicsDB (https://www.proteomicsDB.org) as integral part of the publication. The first project entitled 'human body map' (https://www.proteomicsdb.org/#projects/42) involves the analysis of 36 different human tissues and body fluids. The second...
ORGANISM(S): Homo sapiens (Human) 
2014-05-28 | PXD000865 | Pride
Human proteome Human body map Missing proteins 
Sort   by:  
 Page size