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ProteomicsAnalytical guidelines for co-fractionation mass spectrometry obtained through global profiling of gold standard Saccharomyces cerevisiae protein complexes
Co-fractionation mass spectrometry (CF-MS) is a technique with potential to characterise endogenous and unmanipulated protein complexes on an unprecedented scale. However this potential has been offset by a lack of guidelines for best-practice CF-MS data collection and analysis. To obtain such guide...
ORGANISM(S): Saccharomyces cerevisiae (Baker's yeast) 
2020-08-24 | PXD019513 | Pride
Protein correlation profiling Co-fractionation mass spectrometry Protein complexes Reference complex profiling Protein-protein interactions 
ProteomicsCo-ordinated alternative splicing changes drive osteogenic differentiation of human bone marrow stromal stem cells
Alternative splicing (AS) is fundamental to our understanding of the transcriptional regulation of cell differentiation, yet the dynamic landscape of AS and its regulation during osteoblast differentiation from bone marrow stromal stem cells remains largely uncharacterised. By combining deep RNA seq...
ORGANISM(S): Homo sapiens (Human) 
2026-09-14 | PXD027590 | Pride
Osteogenic differentiation Alternative splicing Single-cell rna sequencing Human bone marrow stromal stem cells 
ProteomicsThe human cholesterogenic enzymes DHCR14 and LBR are differentially regulated transcriptionally and post-translationally
Cholesterol synthesis is a tightly regulated process, both transcriptionally and post-translationally. Transcriptional control of cholesterol synthesis is relatively well understood. However, of the ~20 enzymes in cholesterol biosynthesis, post-translational regulation has only been examined for a s...
ORGANISM(S): Homo sapiens (Human) Cricetulus griseus (Chinese hamster) (Cricetulus barabensis griseus) 
2020-01-15 | PXD016417 | Pride
Dhcr14 Lbr Cholesterol synthesis enzymes 
ProteomicsANP32e binds histone H2A.Z in a cell cycle-dependent manner and regulates its protein stability in the cytoplasm
ANP32e, a chaperone of H2A.Z, is receiving increasing attention because of its link to cancer growth and progression. An unanswered question is whether ANP32e regulates H2A.Z dynamics during the cell cycle; if so, this could have clear implications for the proliferation of cancer cells. Using the hu...
ORGANISM(S): Homo sapiens (Human) 
2024-05-23 | PXD033229 | Pride
Protein stability Epigenetics Chromatin remodeling Anp32e Chaperone H2a.z Cell cycle Chromatin 
ProteomicsA systematic analysis of methylpeptide false discovery rates
All large-scale LC-MS/MS post-translational methylation site discovery experiments require methylpeptide spectrum matches (methyl-PSMs) to be identified at acceptably low false discovery rates (FDRs). To meet estimated methyl-PSM FDRs, methyl-PSM filtering criteria are often determined using the tar...
ORGANISM(S): Saccharomyces cerevisiae (Baker's yeast) 
2016-01-05 | PXD002857 | Pride
Arginine methylation Heavy-methyl silac Lysine methylation False discovery rates Target-decoy approach 
ProteomicsPRMT6 substrate specificity analysis
Protein arginine methyltransferase 6 (PRMT6) catalyses the asymmetric dimethylation of arginines on numerous substrate proteins within the human cell. In particular, PRMT6 methylates histone H3 arginine 2 (H3R2) which affects both gene repression and activation. However, the substrate specificity of...
ORGANISM(S): Homo Sapiens 
PXD016711 | panorama
ProteomicsSILAC analysis of Δhmt1 yeast - Knockout of the Hmt1p arginine methyltransferase in Saccharomyces cerevisiae leads to the dysregulation of phosphate-associated genes and processes
In this study, the proteome-level effects of the deletion of the gene encoding Hmt1p, the predominant yeast arginine methyltransferase, were measured using SILAC (stable isotope labeling by amino acids in cell culture), to clarify if the abundance levels of any proteins were disrupted by systemic lo...
ORGANISM(S): Saccharomyces cerevisiae (Baker's yeast) 
2018-11-21 | PXD004054 | Pride
Methylation Yeast Arginine methylation Hmt1 Phosphate Silac 
UnknownTATA-Box Binding Protein O-GlcNAcylation at T114 Regulates Formation of the B-TFIID Complex and Is Critical for Metabolic Gene Regulation.
Not available
S-EPMC7061307 | biostudies-literature
UnknownThe terminal enzymes of cholesterol synthesis, DHCR24 and DHCR7, interact physically and functionally.
Not available
S-EPMC4373745 | biostudies-literature
UnknownThe influence of biotic and abiotic factors on the bacterial microbiome of gentoo penguins (Pygoscelis papua) in their natural environment.
Not available
S-EPMC11297207 | biostudies-literature
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