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ProteomicsGel-free mass spectrometry analysis of Drosophila melanogaster heads
Here we describe a bottom-up approach to analyse an enriched membrane fraction from Drosophila melanogaster heads using multidimensional liquid chromatography (LC) coupled with tandem-mass spectrometry (MS/MS) that relies on a complete solubilisation and digestion of proteins. An enriched membrane f...
ORGANISM(S): Drosophila melanogaster (Fruit fly) 
2015-07-27 | PXD001712 | Pride
Proteomics Mass spectrometry Heads Membrane proteins Drosophila melanogaster 
Genomicsheads only
heads only
PRJNA87985 | ENA
TranscriptomicsRNA-Seq of Heliconius melpomene heads
We made RNA-Seq libraries of Heliconius melpomene heads, antennae, legs and mouth parts to identify genes upregulated in heads. We explored the expression patterns of a gene family that functions in visual pigment chromophore transport in Drosophila to identify a non-orthologous gene that has a simi...
ORGANISM(S): Heliconius melpomene 
2018-02-28 | E-MTAB-6342 | biostudies-arrayexpress
GenomicsRNA-Seq of Heliconius melpomene heads
RNA-Seq of Heliconius melpomene heads
PRJEB24062 | ENA
GenomicsTotal RNA from different species fly heads
Total RNA from different species fly heads
PRJNA510827 | ENA
TranscriptomicsSex-biased expression in heads of three Drosophila species
Investigation of sex-biased expression across species have relied on measurements from whole flies which sample the extensive expression differences found in the germline and gonads of females and males. We wanted to examine genes with sex-biased expression in a somatic tissue to analyze patterns of...
ORGANISM(S): Drosophila pseudoobscura 
2010-07-30 | E-GEOD-23309 | biostudies-arrayexpress
TranscriptomicsSex-specific gene expression profiles of Aedes aegypti 24 hr pupal heads
Custom microarrays were used to examine global differences in female vs. male gene expression in the developing pupal head of the dengue vector mosquito Aedes aegypti. RNA was extracted from the heads of male and female 24 hr pupae. 20 male or female heads were pooled for each of four replicates. ...
ORGANISM(S): Aedes aegypti 
2014-10-27 | E-GEOD-56521 | biostudies-arrayexpress
TranscriptomicsmiRNA* loading on Ago2
In flies, microRNAs (miRNAs) are synthesized as a short imperfect duplex, where the miRNA is paired to another small RNA, dubbed the "miRNA*". Usually, miRNA*s are degraded in the last steps of miRNA maturation, but we found evidence that some of them can be loaded on the Ago2 protein. Sma...
ORGANISM(S): Drosophila melanogaster 
2010-08-02 | E-GEOD-12462 | biostudies-arrayexpress
ProteomicsIn vivo polycystin-1 interactome using heads of Pkd1 knock-in newborn mice
We generated a new mouse model with three copies of the HA epitope and eGFP knocked-in frame into the endogenous mouse Pkd1 gene by CRISPR/Cas9. The modified allele is fully functional. Using nanobody-coupled beads and large quantities of tissue (8 heads of P1 newborn mice / experiment / condition),...
ORGANISM(S): Mus musculus (Mouse) 
2023-08-08 | PXD035250 | Pride
Interactome Pkd1 knock-in mouse model Polycystin-1 Pkd1 Polycystic kidney disease 
TranscriptomicsTranscription profiling of wild type fly heads (CantonS) and eye-less fly heads (sine occulis)
To identify the eye-enriched genes by comparing the mRNA expression profiles from wild type fly heads (CantonS) and eye-less fly heads (sine occulis).
ORGANISM(S): Drosophila melanogaster 
2012-05-17 | E-MEXP-49 | biostudies-arrayexpress
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