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ProteomicsIdentification of biomarkers for PKD1 using urinary exosomes
Autosomal dominant polycystic kidney disease (ADPKD) is a common cause of ESRD. Affected individuals inherit a defective copy of either the PKD1 or PKD2 gene, encoding the proteins polycystin?1 (PC1) or polycystin?2 (PC2) respectively. PC1 and PC2 are secreted on urinary exosome?like vesicles (ELVs)...
ORGANISM(S): Homo Sapiens (ncbitaxon:9606) 
2016-03-01 | MSV000079547 | MassIVE
ProteomicsAnalysis of PKD1 autophosphorylation in vitro and confirmation of stoichiometric S742 phosphorylation of a recombinant PRKD1 kinase domain
Protein kinase D1 (PRKD1, also referred to as PKD1), has been proposed to undergo autophosphorylation on serine 742 and potentially also on serine 738 in the activation loop of its kinase domain. We used recombinant PKD1 kinase domain to test whether PKD1 is indeed able to undergo autophosphorylatio...
ORGANISM(S): Homo sapiens (Human) 
2019-08-19 | PXD013216 | Pride
Pkd1 Protein kinase d 
TranscriptomicsRNA seq of fluid shear stress treated mouse Pkd1-/- renal epithelial cells
Pkd1-/- renal epithelial cells are exposed to mechanical forces due to flow-induced shear stress within the nephrons. We applied RNA sequencing to get a comprehensive overview of fluid-shear regulated genes and pathways in the immortalized Pkd1-/- renal proximal tubular epithelial cell line. Cells w...
ORGANISM(S): Mus musculus 
2019-03-01 | E-MTAB-6641 | biostudies-arrayexpress
GenomicsPKD1
PKD1 gene and protein study
PRJEB28230 | ENA
ProteomicsIdentification of biomarkers for PKD1 using urinary exosomes
Autosomal dominant polycystic kidney disease (ADPKD) is a common cause of ESRD. Affected individuals inherit a defective copy of either the PKD1 or PKD2 gene, encoding the proteins polycystin?1 (PC1) or polycystin?2 (PC2) respectively. PC1 and PC2 are secreted on urinary exosome?like vesicles (ELVs)...
ORGANISM(S): Homo sapiens (Human) 
2014-12-02 | PXD001075 | Pride
Exosomes Exosome-like vesicles Pkd Adpkd Autosomal dominant polycystic kidney disease Urine 
ProteomicsCellular concentrations of Protein Kinase D1 in HEK293T and INS-1 cells
Protein kinase D1 (PRKD1, also referred to as PKD1), has been proposed to undergo concentration dependent dimerization in vitro. To test whether the cellular concentration of PKD1 is in the range of the dissociation constant obtained, the expression level of PKD1 was investigated by PRM. To this end...
ORGANISM(S): Homo sapiens (Human) Rattus norvegicus (Rat) 
2019-08-19 | PXD013232 | Pride
Pkd1 Ins-1 Protein kinase d Hek293t 
ProteomicsIn vivo polycystin-1 interactome using heads of Pkd1 knock-in newborn mice
We generated a new mouse model with three copies of the HA epitope and eGFP knocked-in frame into the endogenous mouse Pkd1 gene by CRISPR/Cas9. The modified allele is fully functional. Using nanobody-coupled beads and large quantities of tissue (8 heads of P1 newborn mice / experiment / condition),...
ORGANISM(S): Mus musculus (Mouse) 
2023-08-08 | PXD035250 | Pride
Interactome Pkd1 knock-in mouse model Polycystin-1 Pkd1 Polycystic kidney disease 
GenomicsPkd1 and Pkd2 mRNA cis-inhibition drives polycystic kidney disease progression [Pkd1]
Pkd1 and Pkd2 mRNA cis-inhibition drives polycystic kidney disease progression [Pkd1]
PRJNA805542 | ENA
TranscriptomicsDehydration facilitates cyst formation and growth in Pkd1-/- mice via macrophage
The Pkd1 gene was inactivated by tamoxifen at postnatal day 30 (P30) to generate a chronic-onset conditional Pkd1 gene knockout mouse model. Without any intervention, micro cysts could be detected adjacent to renal medulla-corticomedullary junction in Pkd1-/- mice until P180 and cystic phenotype is ...
ORGANISM(S): Mus musculus 
2021-04-27 | E-MTAB-10449 | biostudies-arrayexpress
MetabolomicsDissection of metabolic reprogramming in polycystic kidney disease reveals coordinated rewiring of bioenergetic pathways
Autosomal Dominant Polycystic Kidney Disease (ADPKD) is a genetic disorder caused by loss-of-function mutations in PKD1 or PKD2. Increased glycolysis is a prominent feature of the disease, but how it impacts on other metabolic pathways is unknown. Here, we present an analysis of mouse Pkd1 mutant ce...
2019-06-07 | MTBLS677 | MetaboLights
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