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ProteomicsProMetheus: a database of hmSILAC-validated protein methylation sites
Protein arginine (R) methylation is a post-translational modification that has been shown to play a role in various biological processes, such as RNA splicing, DNA repair, immune response, signal transduction and tumor development. Here, we present a dataset of high-quality methylations obtained fro...
ORGANISM(S): Homo sapiens (Human) 
2022-06-02 | PXD027949 | Pride
Heavy methyl silac Silac Methylation 
ProteomicsDetermining the Mitochondrial Methyl Proteome in Saccharomyces cerevisiae using Heavy Methyl SILAC
"Determining the Mitochondrial Methyl Proteome in Saccharomyces cerevisiae using Heavy Methyl SILAC" Heavy Methyl SILAC proteomics toward characterization and validation of mitochondrial, methylated proteins.
ORGANISM(S): Saccharomyces Cerevisiae (ncbitaxon:4932) 
2016-08-31 | MSV000080107 | MassIVE
ProteomicsHeavy-methyl SILAC of ribosomal proteins
Methylation on ribosome subunits and ribosime-associating proteins were compared in a low- and high-SAM conditions using heavy-methyl SILAC. Ribosome proteins were isolated in two ways; one was ultracentrifugation and the other was anti-FLAG immunoprecipitation using HeLa cells transfected with a FL...
ORGANISM(S): Homo Sapiens (ncbitaxon:9606) 
2022-04-21 | MSV000089296 | MassIVE
UnknownDetermining the Mitochondrial Methyl Proteome in Saccharomyces cerevisiae using Heavy Methyl SILAC.
Not available
S-EPMC5148652 | biostudies-literature
ProteomicsA systematic analysis of methylpeptide false discovery rates
All large-scale LC-MS/MS post-translational methylation site discovery experiments require methylpeptide spectrum matches (methyl-PSMs) to be identified at acceptably low false discovery rates (FDRs). To meet estimated methyl-PSM FDRs, methyl-PSM filtering criteria are often determined using the tar...
ORGANISM(S): Saccharomyces cerevisiae (Baker's yeast) 
2016-01-05 | PXD002857 | Pride
Arginine methylation Heavy-methyl silac Lysine methylation False discovery rates Target-decoy approach 
ProteomicsQuantitative profiling of the enzymatic activity of the protein lysine mono-methyltransferase SMYD2 using SILAC-based proteomics
The significance of non-histone lysine methylation in cell biology and human disease is an emerging area of research exploration, and small molecule inhibitors that selectively and potently target “writers” and “erasers” of methyl-lysine, such as the protein lysine mono-methyltransferase SMYD2, are ...
ORGANISM(S): Homo sapiens (Human) 
2016-01-11 | PXD002405 | Pride
Methylation Lysine methyltransferase Silac Immunoaffinity enrichment Smyd2 
ProteomicsProteomics Profiling of Arginine Methylation Defines PRMT5 Substarate Specificity
Protein arginine methyltransferases (PRMTs) catalyze arginine methylation, an abundant post-translational modification occurring on both chromatin-bound and cytoplasmic proteins. Growing evidence supports the involvement of PRMT5, the major Type II PRMT, in pro-survival and differentiation pathways,...
ORGANISM(S): Homo sapiens (Human) 
2019-04-10 | PXD009070 | Pride
Lc-ms Prmt5 Arginine methylation 
ProteomicsMethyl-SILAF mapping of the Drosophila mitochondrial protein methylome
S-adenosylmethionine (SAM) is the principal biological methyl group donor for a diverse range of substrates. It is synthesised in the cytosolic methionine cycle and shuttled throughout the cell. The mitochondrial SAM (mitoSAM) pool depends on import through the inner-membrane S-adenosylmethionine ca...
ORGANISM(S): Drosophila melanogaster (Fruit fly) 
2021-02-21 | PXD019654 | Pride
Ptm Mitochondria Methylation Drosophila 
ProteomicsCharacterization of Arginine Methylation in Cancer Cell using LC-MS/MS
Protein arginine methyltransferase 9 (PRMT9) activity has been observed to be elevated in cancer patients, including many types of leukemias, correlating with poor prognosis and decreased response to immune checkpoint inhibitors. By targeting PRMT9, we can eliminate PRMT9-proficient/immune-cold canc...
ORGANISM(S): Homo sapiens (Human) 
2024-01-11 | PXD039441 | Pride
Lc-ms/ms 
ProteomicsPhosphoproteomic profiling of the DNA damage response in MCF10A and human PBMC cells by SILAC labeling and 1D and 2D-LC-MS/MS
The DNA-damage response (DDR) is a critical network for maintaining genomic integrity, and mutations in the DDR are among the most frequently identified in tumors. Phosphorylation is a key signaling event in the DDR network. We sought to design high throughput, mass spectrometry based assays to moni...
ORGANISM(S): Homo sapiens (Human) 
2015-12-01 | PXD002363 | Pride
Human Pbmc Silac Ir Mms Mcf10a Lc-ms/ms 
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