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ProteomicsPhosphoproteomic analysis of T cell receptor stimulation-induced changes in human primary CD4+CD25- T cells in the absence or presence of regulatory T cells.
This is an addition to dataset PXD004291. This data were only used for confirmation of certain phosphopetides. The dataset was not included in inital analysis due to suboptimal sample preparation.
ORGANISM(S): Homo sapiens (Human) 
2017-08-28 | PXD006142 | Pride
T-cells Phosphoproteomics 
ProteomicsPhosphoproteomic analysis of T cell receptor stimulation-induced changes in human primary CD4+CD25- T cells in the absence or presence of regulatory T cells.
Regulatory T cells (Tregs) suppress other immune cells and are indispensable for human health, yet the molecular mechanisms of suppression remain incompletely understood. Tregs can suppress proliferation and cytokine production of CD4+CD25- conventional T cells (Tcons). We previously demonstrated th...
ORGANISM(S): Homo sapiens (Human) 
2017-08-28 | PXD004291 | Pride
Co-cultures Regulatory t cells (tregs) Ti-imac Human cd4+ t cells Phosphoproteomics 
ProteomicsTi4+-IMAC label-free quantification
We combine high-resolution mass spectrometry with Ti4+-IMAC phosphopeptide enrichment and label-free quantification to monitor the phosphoproteome of Jurkat T-cells following stimulation by Prostaglandin E2. Jurkat T lymphoma cells were grown in RPMI 1640 medium supplemented with 10% fetal bovine se...
ORGANISM(S): Homo sapiens (Human) 
2014-05-22 | PXD000293 | Pride
Label-free phosphoproteomics 
ProteomicsMultiple protease based human phosphopeptide atlas
Site-specific phospho-antibodies are commonly used to study these changes, however only a limited number of good antibodies is available, covering just a few signaling nodes in important pathways. It is highly unlikely that specific antibodies, covering the more than hundred thousands of estimated p...
ORGANISM(S): Homo Sapiens (ncbitaxon:9606) 
2016-10-04 | MSV000080223 | MassIVE
ProteomicsProteomic analysis of T cell differentiation
T helper cells (Th) play an important role guarding, regulating and modulating immune responses. The different Th lineages fulfill different tasks in response to infections. The two best defined subtypes are Th1 (involved in cellular immunity) and Th2 (humoral immunity). The cytokine environment aft...
ORGANISM(S): Mus musculus (Mouse) 
2019-02-14 | PXD004532 | Pride
Th2 Th1 Murine cd4+ t- cells Q exactive Scx 
ProteomicsExtending proteome coverage through an on-line 2D SCX-RP ultra-high pressure system
Ultra high pressure liquid chromatography (UHPLC) systems combined with state of the art mass spectrometers have pushed the limit of deep proteome sequencing to new heights making it possible to identify thousands of proteins in a single LC/MS experiment within a few hours. However, the relationship...
ORGANISM(S): Homo Sapiens (ncbitaxon:9606) 
2017-03-31 | MSV000080845 | MassIVE
ProteomicsPersonalized proteome profiles of healthy and tumor human colon organoids reveal both individual diversity and basic features of colorectal cancer.
We performed quantitative mass spectrometry based proteomic analysis and comparative transcriptomics analysis of 7 individual patient-derived human colorectal tumor and healthy organoids, the latter acquired from adjacent healthy tissue.
ORGANISM(S): Homo sapiens (Human) 
2017-01-16 | PXD004149 | Pride
Proteomics Organoids Colon cancer 
ProteomicsErythrocyte soluble proteome analysis in hemolytic anaemia patients
We investigated the RBCs of a hemolytic anemia patient using quantitative proteomics. We used stable isotope dimethyl labeling to accurately quantify the RBC proteins. As controls, 1) samples of four healthy subjects were taken to account for normal variation in healthy individuals, and 2) samples...
ORGANISM(S): Homo sapiens (Human) 
2016-09-27 | PXD001837 | Pride
Proteomics Hemolytic anema Red blood cell 
ProteomicsMultiple protease based human phosphopeptide atlas
Site-specific phospho-antibodies are commonly used to study these changes, however only a limited number of good antibodies is available, covering just a few signaling nodes in important pathways. It is highly unlikely that specific antibodies, covering the more than hundred thousands of estimated p...
ORGANISM(S): Homo sapiens (Human) 
2015-05-28 | PXD001428 | Pride
Mass spectrometry Proteases Kinase Phosphorylation 
ProteomicsThe interactome of BTN3A1 (Butyrophilin Subfamily 3 Member A1)
In this study we dissected the BTN3A1 interactome before or after the treatment with pamidronate to gain mehcanistic insights into the gamma9/delta2 T cell dependent recognition of target cells. Our approach relied on affinity purification couple with mass spectrometry from a BTN3A knock-out cell li...
ORGANISM(S): Homo sapiens (Human) 
2020-06-05 | PXD018055 | Pride
Btn3a1 Ap-ms 
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