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ProteomicsC. difficile strain 630 flagellin C modifications
In proteomics, the study of post-translational modifications often relies on some type of enrichment strategy. Here, we show that an approach that is commonly used in phosphoproteomics (Immobilised Metal Affinity Chromatography (Fe3+-IMAC)) also enriches for peptides with a unique post-translational...
ORGANISM(S): Peptoclostridium difficile (strain 630) (Clostridium difficile) 
2022-04-04 | PXD029552 | Pride
C. difficile Phosphoproteomics Flagellin 
ProteomicsRetinal proteomics of a mouse model of dystroglycanopathies shows changes in different photoreceptor regions
Mutations in the POMT1 gene, encoding a protein O-mannosyltransferase essential for alpha-dystroglycan (α-DG) glycosylation, are frequently observed in a group of rare congenital muscular dystrophies, collectively known as dystroglycanopathies. However, it is hitherto unclear whether the effects se...
ORGANISM(S): Mus musculus (Mouse) 
2021-09-10 | PXD023704 | Pride
Mouse model Dystroglycanopathies Photoreceptor 
ProteomicsClostridioides difficile phosphoproteomics shows an expansion of phosphorylated proteins in stationary growth phase
Phosphorylation is a post-translational modification that can affect both house-keeping functions and virulence characteristics in bacterial pathogens. In the Gram-positive enteropathogen Clostridioides difficile the extent and nature of phosphorylation events is poorly characterized, though a prote...
ORGANISM(S): Peptoclostridium difficile (strain 630) (Clostridium difficile) 
2022-02-16 | PXD029475 | Pride
Clostridioides difficile Phosphoproteomics 
ProteomicsIn-depth specificity profiling of Pro-Pro endopeptidases (PPEPs) using combinatorial synthetic peptide libraries
Proteases comprise the class of enzymes that catalyze the hydrolysis of peptide bonds, thereby playing a pivotal role in many aspects of life. The amino acids surrounding the scissile bond determine the susceptibility towards protease-mediated hydrolysis. A detailed understanding of the cleavage spe...
ORGANISM(S): Paenibacillus alvei DSM 29 Geobacillus stearothermophilus Clostridioides difficile 
2023-08-01 | PXD038277 | Pride
Pro-pro endopeptidase Specificity profiling Mass spectrometry (ms) Ppep Synthetic peptide libraries Substrate specificity High-performance liquid chromatography (hplc) Fret peptides Metalloprotease 
ProteomicsComplement is activated by elevated IgG3 hexameric platforms and deposits C4b onto distinct antibody domains
IgG3 is unique among the IgG subclasses due to its extended hinge, allotypic diversity and enhanced effector functions, including highly efficient pathogen neutralization and complement activation. It is also underrepresented as an immunotherapeutic candidate, partly due to a lack of structural info...
ORGANISM(S): Homo sapiens (Human) 
2023-10-24 | PXD039049 | Pride
Igg Cryoem Igg3 
ProteomicsOxonium ion guided analysis of quantitative proteomics data reveals site-specific O-glycosylation of anterior gradient protein 2 (AGR2)
Developments in mass spectrometry (MS)-based analyses of glycoproteins have been important to study changes in glycosylation related to disease. Recently, the characteristic pattern of oxonium ions in glycopeptide fragmentation spectra had been used to assign different sets of glycopeptides. In part...
ORGANISM(S): Homo sapiens (Human) 
2022-02-17 | PXD025805 | Pride
Glycoproteomics  tmt labeling  oxonium ion  lc−ms/ms  o-glycosylation  colorectal cancer  agr2 
ProteomicsN-glycoproteins have a major role in MGL binding to colorectal cancer cell lines
Colorectal cancer (CRC) is the second leading cause of cancer death worldwide due in part to a high proportion of patients diagnosed at advanced stages of the disease. For this reason, many efforts have been made towards new approaches for early detection and prognosis. Cancer-associated aberrant gl...
ORGANISM(S): Homo sapiens (Human) 
2020-08-10 | PXD020344 | Pride
Glycoproteomics  tmt labelling  c-type lectin  colorectal cancer  lacdinac  tn antigen 
ProteomicsGlycoproteomic analysis of MGL binding protein on acute T cell leukemia cells
O-glycosylation is generally initiated by the transfer of a N-acetylgalactosamine to Ser/Thr residues of proteins, forming the Tn antigen. This truncated surface glycan is expressed at high levels by tumor cells and is associated with higher metastatic behaviour and poor prognosis of patients. The T...
ORGANISM(S): Homo sapiens (Human) 
2019-01-02 | PXD011307 | Pride
Glycoproteomics Leukemia O-glycosylation Tn-antigen Lectin 
ProteomicsPTX3 structure determination using a hybrid cryo-electron microscopy and AlphaFold approach offers insights into ligand binding and complement activation
Pattern recognition molecules (PRMs) form an important part of innate immunity and respond to infection and damage via triggering processes such as inflammation. The pentraxin family of soluble PRMs comprises long and short pentraxins, with the former containing unique N-terminal regions unrelated t...
ORGANISM(S): Homo sapiens (Human) 
2022-10-14 | PXD034602 | Pride
Ptx3 
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