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MetabolomicsSodium Butyrate mediates Histone Crotonylation and Alleviated Neonatal Rats Hypoxic-Ischemic Brain Injury Through Gut-Brain Axis (untargeted fecal metabolomics)

Neonatal hypoxic-ischemic encephalopathy (HIE) refers to nervous system damage caused by perinatal hypoxia, which is the major cause of long-term neuro-developmental disorders in surviving infants. However, the mechanisms still require further investigation. In this study, we found that the butan...

2022-10-12 | MTBLS4932 | MetaboLights
MetabolomicsSodium Butyrate mediates Histone Crotonylation and Alleviated Neonatal Rats Hypoxic-Ischemic Brain Injury Through Gut-Brain Axis (targeted SCFA brain metabolomics)

Neonatal hypoxic-ischemic encephalopathy (HIE) refers to nervous system damage caused by perinatal hypoxia, which is the major cause of long-term neuro-developmental disorders in surviving infants. However, the mechanisms still require further investigation. In this study, we found that the butan...

2022-10-12 | MTBLS4933 | MetaboLights
MetabolomicsHistone H3K4me3 Demethylase RBP2 Transcriptionally Activates PPARγ to Drive Lipid Metabolic Reprogramming in Gastric Carcinoma

This work investigates epigenetic driven lipid metabolic reprogramming in gastric carcinoma. We characterize that histone H3K4me3 demethylase RBP2 transcriptionally activates PPARgama by binding to its promoter region,thereby promoting lipogenic gene expression and intracellular lipid accumulatio...

2026-08-14 | MTBLS15324 | MetaboLights
ProteomicsArg-C Ultra simplifies histone preparation for LC-MS/MS
Using mammalian histone extract, we demonstrate that Arg-C Ultra facilitates histone preparation for LC-MS/MS. We show the performance of Arg-C Ultra in terms of digestion specificity, number of modified forms identified, and yield of quantitative information compared with standard Arg-C and trypsin...
ORGANISM(S): Homo sapiens (Human) 
2025-06-30 | PXD059514 | Pride
Histone Arg-c ultra Trimethylacetic anhydride Lc-ms/ms Chemical derivatization 
ProteomicsMicrowave oven-assisted derivatization of histone proteoforms using trimethylacetic anhydride
We introduce a new reagent – Trimethylacetic anhydride (TMA) for highly efficient histone derivatization, which is facilitated by microwave irradiation to reduce sample preparation duration. The derivatization of unmodified NH2- groups at histone lysine residues is necessary prior LC-MS analysis usi...
ORGANISM(S): Homo sapiens (Human) 
2021-06-14 | PXD019502 | Pride
O histone derivatization Epigenetics Mass spectrometry Trimethylacetic anhydride 
ProteomicsSample preparation procedure for mass spectrometric analysis of plant histones
The protocol for preparing samples of plant histones prior to LC-MS/MS analysis was established, using a filter-aided sample preparation (FASP) technique to remove natural contaminants and chemical additives incompatible with MS-based procedures. The protocol consists of nuclei isolation, histone ex...
ORGANISM(S): Arabidopsis thaliana (Mouse-ear cress) 
2018-09-20 | PXD010102 | Pride
Arabidopsis thaliana Mass spectrometry Post-translational modifications Epigenetics Histone derivatization Filter-aided sample preparation 
ProteomicsComparison of in-gel digestion methods for histone PTM analysis
In order to optimize the histone digestion procedure for low-abundance clinical samples, we compared different in-gel digestion protocols. Histones are usually digested in-gel using “Arg-C-like” strategies, which involve chemical acylation of lysines followed by trypsin digestion (17). Acylated lysi...
ORGANISM(S): Homo sapiens (Human) 
2021-09-09 | PXD024745 | Pride
Epigenetics Histone post-translationa modification 
ProteomicsAdaptive epigenetic changes in histone variants and modifications rescue the fitness of Arabidopsis plants with dysfunctional histone chaperones
The aim was to characterize histone variants and PTMs in mutant plants with histone chaperone dysfunction using mass spectrometry-based approach. We found that the maintenance of genome stability via chromatin remodelling depends on a two-tiered process. The first level relies on a highly flexible m...
ORGANISM(S): Arabidopsis thaliana (Mouse-ear cress) 
2024-08-10 | PXD046034 | Pride
Arabidopsis Immunochemistry. Post-translational modifications Mass spectrometry Histone chaperone complex Chromatin remodeling Histone variants 
ProteomicsImproved Mass Spectrometry-based methods to study histone propionylation
Histone post-translational modifications (PTMs) have crucial roles in a multitude of cellular processes, their aberrant levels have been linked with numerous diseases, including cancer. Although histone PTM investigations have focused so far on methylations and acetylations, alternative long-chain a...
ORGANISM(S): Homo sapiens (Human) Mus musculus (Mouse) 
2024-07-11 | PXD050362 | Pride
Histone post-translational modifications Propionylation Acylation 
ProteomicsBreaking Boundaries in Histone Modification MS-based Detection: A Tailored Search Strategy for Unrestricted identification of novel epigenetic marks
Histone post-translational modifications (PTMs) are crucial for gene expression regulation and DNA maintenance, and their aberrations are linked to multiple diseases, like cancer. While functions related to lysine acetylation and methylation have been well-studied, recent studies have uncovered addi...
ORGANISM(S): Homo sapiens (Human) 
2025-11-11 | PXD061934 | Pride
Human Lc-msms Bioinformatics Epigenetics Histone post-translational modifications Open search 
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