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GenomicsDistribution of active (H3K4me1, H3K4me3, H3K27ac) and inactive (H3K9me3, H3K27me3) histone PTMs in WT and HIRA KO HeLa cells
We profiled the enrichment of active (H3K4me1, H3K4me3, H3K27ac) and inactive (H3K9me3, H3K27me3) histone PTMs in cells that are wild-type (WT) or knock-out (KO) for the H3.3-specific chaperone HIRA. We performed native ChIP-seq following MNase digestion to isolate nucleosomes in H3.1-SNAP and H3.3-...
ORGANISM(S): Homo sapiens 
2025-08-07 | E-MTAB-14416 | biostudies-arrayexpress
ProteomicsSalinity-responsive histone PTMs identified in Mozambique tilapia tissues
Histone post-translational modifications (PTMs) are epigenetic marks that can be induced by environmental stress and elicit heritable patterns of gene expression. To investigate this process in an ecological context, we characterized the influence of salinity stress on histone PTMs within the gills,...
ORGANISM(S): Oreochromis Mossambicus 
PXD040557 | panorama
ProteomicsTwo distinct modes for propagation of histone PTMs across the cell cycle
Epigenetic states defined by chromatin can be maintained through mitotic cell division. However, it remains unknown how histone-based information is transmitted. Here we combine nascent chromatin capture (NCC) and triple-SILAC labelling to track histone modifications and histone variants during DNA ...
ORGANISM(S): Homo sapiens (Human) 
2015-03-30 | PXD001980 | Pride
Ncc  histone modifications  inheritance  chromatin  epigenetics 
ProteomicsQuantifying histone PTMs by bottom-up MS
Histone post-translational modifications (PTMs) contribute to chromatin function through their chemical properties which influence chromatin structure, and their ability to recruit chromatin interacting proteins. Nanoflow liquid chromatography coupled with high resolution tandem mass spectrometry (n...
ORGANISM(S): Homo sapiens (Human) 
2015-03-27 | PXD001541 | Pride
Proteomics Post-translational modification Isobaric peptide Retention time Histone Quantification 
ProteomicsA strategy to characterize the global histone PTM landscape within tissues of non-model organisms
Histone post-translational modifications (PTMs) are epigenetic marks that play a critical role in the expression and maintenance of DNA, but they remain largely uninvestigated in non-model organisms due to technical challenges. To begin alleviating this issue, we developed a workflow for histone PTM...
ORGANISM(S): Oreochromis Mossambicus 
2024-02-29 | PXD040536 | panorama
ProteomicsAnalysis of histone PTMs in nascent chromatin in new and old histones upon DNA damage (CAL353, CAL358) using the NCC-SILAC approach
Dynamic recycling and de novo deposition of histones are fundamental for chromatin restoration. Histone post-translational modifications (PTMs) are assumed to have a causal role in establishing distinct chromatin structures. However, it remains unknown how specific histone PTMs are regulated at brok...
ORGANISM(S): Homo sapiens (Human) 
2021-01-18 | PXD018220 | Pride
Ncc Histone ptms Silac Camptothecin Replication 
ProteomicsEnvironmental Conditions Elicit a Slow but Enduring Response of Histone Post-Translational Modifications in Mozambique Tilapia
Histone post-translational modifications (PTMs) are epigenetic marks that operate within the central dogma of molecular biology: upon an environmental stimulus, the histone PTMs surrounding DNA can be changed in a way that modifies gene expression, and, therefore, the abundance and composition of RN...
ORGANISM(S): Oreochromis Mossambicus 
2024-12-10 | PXD042718 | panorama
ProteomicsHistone modifications associated with the histone chaperone DAXX
A multitude of histone chaperones is required to protect histones from their biosynthesis to DNA deposition. They cooperate through the formation of co-chaperone complexes, but the crosstalk between nucleosome assembly pathways is unclear. Using explorative interactomics approaches, we map the organ...
ORGANISM(S): Mus musculus (Mouse) 
2023-03-06 | PXD034924 | Pride
Daxx Ptms H3.3 Histones 
ProteomicsImprovements on the quantitative analysis of Trypanosoma cruzi histone post translational modifications
Trypanosome histone N-terminal sequences are very divergent from the other eukaryotes, although they are still decorated by post-translational modifications (PTMs). Here, we used a highly robust workflow to analyze histone PTMs in the parasite Trypanosoma cruzi using mass spectrometry-based data-ind...
ORGANISM(S): Trypanosoma cruzi cruzi 
2022-02-07 | PXD018295 | Pride
Ptm Trypanosoma cruzi Histone 
ProteomicsDevelopment of a high-throughput (10 minute microflow gradient) platform for quantitation of histone modifications on a new QTOF instrument
Histone post-translational modifications (PTMs) regulate gene expression patterns through epigenetic mechanisms. The 5 histone proteins (H1, H2A, H2B, H3, and H4) are extensively modified, with over 75 distinct modification types spanning more than 200 sites. Despite strong advances in mass spectrom...
ORGANISM(S): Homo Sapiens (ncbitaxon:9606) 
2024-05-06 | MSV000094696 | MassIVE
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