Sort   by:  
 Page size 
ProteomicsHIV-1 Nef changes the proteome of T cells extracellular vesicles
Extracellular vesicles (EVs) are biomolecule carriers for intercellular communication in health and disease. Nef is a human immunodeficiency virus (HIV) virulence factor that is released from cells within EVs and is present in plasma EVs of HIV-1 infected individuals. We performed a quantitative pro...
ORGANISM(S): Bos taurus (Bovine) Homo sapiens (Human) 
2024-01-26 | PXD041028 | Pride
Hiv-1 Nef Exosomes Extracelular vesicles 
TranscriptomicsTranscription profiling by array of human CD4+ T-lymphocytes infected with VSVG-pseudotyped HIV-1 viruses lacking Env, Vpr, and Nef
The high mutation rate of HIV is linked to the generation of viruses expressing proteins with altered function whose impact on disease progression is unknown. We investigated the effects of HIV-1 viruses lacking Env, Vpr and Nef on CD4+ T cell gene expression using high-density DNA microarray analys...
ORGANISM(S): Homo sapiens 
2008-10-26 | E-GEOD-12963 | biostudies-arrayexpress
TranscriptomicsImproved Innate and Adaptive Immunostimulation by Genetically Modified HIV-1 Protein Expressing NYVAC Vectors
Attenuated poxviruses are safe and capable to express foreign antigens. Poxviruses are applied in veterinary vaccination and explored as candidate vaccines for humans. However, poxviruses express multiple genes encoding proteins that interfere with components of the innate and adaptive immune respon...
ORGANISM(S): Homo sapiens 
2010-12-21 | E-GEOD-26239 | biostudies-arrayexpress
TranscriptomicsTranscription profiling of Jurkat CF4+ T cells transfected with simian immunodeficiency virus Nef cells leads to a molecular profile of viral persistence and immune evasion.
Jurkat T cell line was transfected with SIV Nef controlled by an inducible promoter system. Nef was induced by addition of 10 micromolar pronasterone A and gene expression values were determined after 24 hrs. Control Jurkat cells were untransfected and treated with 10 micromolar pronasterone A and g...
ORGANISM(S): Homo sapiens 
2007-10-26 | E-GEOD-4785 | biostudies-arrayexpress
TranscriptomicsInterplay between HIV-1 infection and host microRNAs
Using microRNA array analyses of in vitro HIV-1-infected CD4+ cells, we find that several host microRNAs are significantly up- or downregulated around the time HIV-1 infection peaks in vitro. While microRNA-223 levels were significantly enriched in HIV-1-infected CD4+CD8? PBMCs, microRNA-29a/b, micr...
ORGANISM(S): Homo sapiens 
2012-12-05 | E-GEOD-42737 | biostudies-arrayexpress
TranscriptomicsGene expression profiling of CD4+ T cell line due to presence of Nef during HIV-1 infection.
We have utilized microarray technology to compare gene expression profile in presence or absence of Nef during HIV-1 infection in T cell-line. We found several genes significantly differentially regulated due to ∆nef HIV-1 infection and WT HIV-1 infection when compared to Uninfected cells and also s...
ORGANISM(S): Homo sapiens 
2016-12-06 | GSE76793 | GEO
ProteomicsProteomic Organellar Mapping Identifies Modulation of Peroxisomes by HIV-1
The interaction between HIV-1 and the host cell has been studied at many levels, but cell-wide organellar changes caused by the virus have not been defined. Here, we aimed to detail perturbations to the host cell spatial proteome following HIV protein expression. Subcellular fractionation followed b...
ORGANISM(S): Homo sapiens (Human) 
2022-01-28 | PXD024716 | Pride
Lc-ms/ms Organelle mapping Proteomics Hiv-1 
ProteomicsNefCD4AP2 Complex Crosslinking with DSSO for Integrative Structure Determination
Objective: HIV-1 Nef suppresses immune surveillance mechanisms to promote viral pathogenesis. Cellular receptor CD4 is essential for HIV entry into host cells, though becomes problematic at later stages in the virus replication cycle. CD4 at the plasma membrane is targeted by Nef for endocytosis an...
ORGANISM(S): Homo sapiens (Human) Human immunodeficiency virus 1 
2021-09-09 | PXD019338 | Pride
Xlms Dsso Cd4 Ap2 Hiv-1 nef 
GenomicsExtracellular vesicles carrying HIV-1 Nef induce long-term hyperreactivity of myeloid cells [RNA-Seq]
Extracellular vesicles carrying HIV-1 Nef induce long-term hyperreactivity of myeloid cells [RNA-Seq]
PRJNA887795 | ENA
TranscriptomicsRole of Cofilin 1 pathway and actin dynamics in nuclear retinoid receptor function
We have investigated the role of actin dynamics and the effect of actin cytoskeleton modifying agents on retinoid receptor-mediated transactivation. Using Nef, an actin modifying HIV-1 protein, the role of LMK1/CFL1-mediated actin dynamics in receptor function was studied. The effect of Nef expressi...
ORGANISM(S): Homo sapiens 
2011-08-25 | E-GEOD-31665 | biostudies-arrayexpress
Sort   by:  
 Page size