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Extracellular vesicles (EVs) are biomolecule carriers for intercellular communication in health and disease. Nef is a human immunodeficiency virus (HIV) virulence factor that is released from cells within EVs and is present in plasma EVs of HIV-1 infected individuals. We performed a quantitative pro...
ORGANISM(S): Bos taurus (Bovine) Homo sapiens (Human) 
2024-01-26 | PXD041028 | Pride
Jurkat T cell line was transfected with SIV Nef controlled by an inducible promoter system. Nef was induced by addition of 10 micromolar pronasterone A and gene expression values were determined after 24 hrs. Control Jurkat cells were untransfected and treated with 10 micromolar pronasterone A and g...
ORGANISM(S): Homo sapiens 
The high mutation rate of HIV is linked to the generation of viruses expressing proteins with altered function whose impact on disease progression is unknown. We investigated the effects of HIV-1 viruses lacking Env, Vpr and Nef on CD4+ T cell gene expression using high-density DNA microarray analys...
ORGANISM(S): Homo sapiens 
Jurkat T cell line was transfected with SIV Nef controlled by an unducible promoter system. Nef was induced by addition of 10 micromolar pronasterone A and gene expression values were determined after 24 hrs. Control Jurkat cells were untransfected and treated with 10 micromolar pronasterone A and g...
ORGANISM(S): Homo sapiens 
2006-07-25 | GSE4785 | GEO
we performed the analysis of SOX9-GFP+ cells purified from mouse embryonic testis at embryonic day E17.5 and Postnatal day 5 and analyzed the effect of the deletion of the Insulin receptor (InsR) and Igf1 receptor (Igf1R) by comparing double mutant and control SOX9+ Sertoli cells
ORGANISM(S): Mus musculus 
HIV Nef transfected HEK293T cell culture and HIV mock transfected HEK293T cell culture [mRNA]
HIV Nef transfected HEK293T cell culture and HIV mock transfected HEK293T cell culture [miRNA]
We have investigated the role of actin dynamics and the effect of actin cytoskeleton modifying agents on retinoid receptor-mediated transactivation. Using Nef, an actin modifying HIV-1 protein, the role of LMK1/CFL1-mediated actin dynamics in receptor function was studied. The effect of Nef expressi...
ORGANISM(S): Homo sapiens 
Objective: HIV-1 Nef suppresses immune surveillance mechanisms to promote viral pathogenesis. Cellular receptor CD4 is essential for HIV entry into host cells, though becomes problematic at later stages in the virus replication cycle. CD4 at the plasma membrane is targeted by Nef for endocytosis an...
ORGANISM(S): Homo sapiens (Human) Human immunodeficiency virus 1 
2021-09-09 | PXD019338 | Pride
Purpose: Comparison of RNA-sequencing datasets obtained from exosomes of Nef-transfected and Mock-transfected HEK293T cells Methods: Assessment of RNA content of exosomes produced by Nef-transfected HEK293T cells and and Mock-transfected HEK293T cells Results: Differences in a set of microRNAs Concl...
ORGANISM(S): Homo sapiens 
2018-11-18 | GSE122656 | GEO
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