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ProteomicsHost Cell Protein Quantification Workflow Using Optimized Standards combined with Data-Independent Acquisition Mass Spectrometry
Monitoring of host cell proteins (HCPs) during the manufacture of monoclonal antibodies (mAb) has become a critical requirement to provide effective and safe drug product. ELISA assays are still the current gold standard for the quantification of protein impurities. However, this technique has sever...
ORGANISM(S): Cricetulus griseus (Chinese hamster) (Cricetulus barabensis griseus) 
2023-07-20 | PXD029305 | Pride
Hcp profiler Protein quantification Data validation. Data-independent acquisition (dia) Host cell proteins (hcps) 
ProteomicsCarrier protein-enabled host cell proteome analysis (CP-HCP) with integrated isobaric tag interference filtering
We have created a CP-HCP method to characterise the carrier proteome of a host cell expression system producing recombinant protein biotherapeutic, which can be compared with the testing sample mixture containing trace amounts of host cell proteins against high-background recombinant protein and als...
ORGANISM(S): Escherichia coli 
2026-01-12 | PXD050214 | Pride
Psms Host cell proteins Ms3 Tmt Carrier proteome Multiplexed proteomics Lc-ms Co-isolation Interference Co-fragmentation Dda 
ProteomicsProspective ICH Q2(R2)-aligned validation of label-free untargeted proteomics for host cell protein quantification in biotherapeutics
This dataset contains raw and processed LC–MS/MS data generated for a prospective validation of label-free untargeted proteomics for host cell protein (HCP) quantification in biotherapeutic matrices. A stable isotope-labeled CHO whole-proteome standard was spiked into the NIST monoclonal antibody re...
ORGANISM(S): Mus musculus (Mouse) 
2026-06-08 | PXD075694 | Pride
Host cell proteins Hcp quantification Biotherapeutics Timstof Analytical validation Untargeted proteomics Ddapasef Ich q2(r2) Lc–ms/ms 
ProteomicsTargeted Quantitation of Host Cell Proteins by Capillary LC/IMS/MS/MS in Combination with Rapid Digestion on Immobilized Trypsin Column Under Native Conditions
Host cell protein (HCP) impurities are considered a critical quality attribute of biopharmaceuticals because of their potential to compromise safety and efficacy, and LC/MS-based analytical methods have been developed to identify and quantify individual proteins instead of employing enzyme-linked im...
ORGANISM(S): Cricetulus Griseus 
PXD053135 | JPOST Repository
ProteomicsA Sensitive, Rapid, Robust and Reproducible Workflow for Host Cell Protein Profiling in Biopharmaceutical Process Development
There is a growing industry and regulatory need to detect host cell protein (HCP) impurities in the production of protein biopharmaceuticals, as certain HCPs can impact product stability, safety, and efficacy, even at low levels.1-6 In some cases, regulatory agencies require the identification as we...
ORGANISM(S): Mus musculus (Mouse) 
2020-06-02 | PXD018427 | Pride
Monoclonal antibody (mab) Drug product (dp) Evosepone Drug substance (ds) Host cell proteins (hcps) Lc-ms/ms 
ProteomicsAdvanced mass spectrometry workflows for accurate quantification of trace-level host cell proteins in drug products: benefits of FAIMS separation and gas-phase fractionation DIA
In this study, we investigated the benefits of adding high-field asymmetric ion mobility spectrometry (FAIMS) separation prior to data dependent acquisition (DDA) and gas phase fractionation (GPF) prior to data independent acquisition (DIA) LC-MS/MS analysis. Native digestion followed by LC-MS/MS wi...
ORGANISM(S): Mus musculus (Mouse) Cricetulus griseus (Chinese hamster) (Cricetulus barabensis griseus) 
2023-05-07 | PXD039582 | Pride
Data-independent analysis (dia) Host cell proteins (hcp) Final drug products (dp) 
ProteomicsAdvanced mass spectrometry workflows for accurate quantification of trace-level host cell proteins in drug products: benefits of FAIMS separation and gas-phase fractionation DIA
In this study, we investigated the benefits of adding high-field asymmetric ion mobility spectrometry (FAIMS) separation prior to data dependent acquisition (DDA) and gas phase fractionation (GPF) prior to data independent acquisition (DIA) LC-MS/MS analysis. Native digestion followed by LC-MS/MS wi...
ORGANISM(S): Mus musculus (Mouse) Cricetulus griseus (Chinese hamster) (Cricetulus barabensis griseus) 
2023-05-07 | PXD039585 | Pride
Faims Data-dependent analysis (dda) Host cell proteins (hcp) Final drug products (dp) 
ProteomicsCarrier protein-enabled host cell proteome analysis (CP-HCP) with integrated isobaric tag interference filtering
We used TMT labelling to set up different experiment channels containing mixtures of the host cell carrier proteome (E coli) and mock biotherapeutic (BSA), to compare with interference detection channel to model and filter interference, a phenomenon where host cell proteins (HCPs) co-elute with high...
ORGANISM(S): Escherichia coli 
2026-01-12 | PXD049970 | Pride
Boost Host cell proteins Multi-plexed proteomics Tmt Carrier proteome Lc-ms Co-isolation Interference Co-fragmentation Dda 
ProteomicsThe host cell proteome of Physcomitrella patens
Host cell proteins are inevitable contaminants of biopharmaceuticals. Here, we performed detailed analyses of the host cell proteome of moss (Physcomitrella patens) bioreactor supernatants using mass spectrometry and subsequent bioinformatics analysis. Distinguishing between the apparent secretome a...
ORGANISM(S): Physcomitrella patens subsp. patens (Moss) 
2018-09-19 | PXD009517 | Pride
Bioreactor Physcomitrella patens Host cell proteome 
ProteomicsQuantitative Host Cell Protein Analysis across Innovator Monoclonal Antibody-Based Protein Therapeutics using the Orbitrap Astral Mass Spectrometer
Protein biotherapeutics such as monoclonal antibodies (mAbs) are generally produced using cell-based production systems that generate endogenous proteins, commonly referred to as host cell proteins (HCPs), along with the desired drug products. A critical challenge is the removal of HCPs from the fin...
ORGANISM(S): Mus musculus (Mouse) Cricetulus griseus (Chinese hamster) (Cricetulus barabensis griseus) 
2026-04-20 | PXD067004 | Pride
Host cell proteins Mab based therapeutics Orbitrap astral ms Mass spectrometry 
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