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ProteomicsCarrier protein-enabled host cell proteome analysis (CP-HCP) with integrated isobaric tag interference filtering
We used TMT labelling to set up different experiment channels containing mixtures of the host cell carrier proteome (E coli) and mock biotherapeutic (BSA), to compare with interference detection channel to model and filter interference, a phenomenon where host cell proteins (HCPs) co-elute with high...
ORGANISM(S): Escherichia coli 
2026-01-12 | PXD049970 | Pride
Boost Host cell proteins Multi-plexed proteomics Tmt Carrier proteome Lc-ms Co-isolation Interference Co-fragmentation Dda 
ProteomicsTargeted Quantitation of Host Cell Proteins by Capillary LC/IMS/MS/MS in Combination with Rapid Digestion on Immobilized Trypsin Column Under Native Conditions
Host cell protein (HCP) impurities are considered a critical quality attribute of biopharmaceuticals because of their potential to compromise safety and efficacy, and LC/MS-based analytical methods have been developed to identify and quantify individual proteins instead of employing enzyme-linked im...
ORGANISM(S): Cricetulus Griseus 
2025-05-02 | PXD053135 | JPOST Repository
ProteomicsHost Cell Protein Quantification Workflow Using Optimized Standards combined with Data-Independent Acquisition Mass Spectrometry
Monitoring of host cell proteins (HCPs) during the manufacture of monoclonal antibodies (mAb) has become a critical requirement to provide effective and safe drug product. ELISA assays are still the current gold standard for the quantification of protein impurities. However, this technique has sever...
ORGANISM(S): Cricetulus griseus (Chinese hamster) (Cricetulus barabensis griseus) 
2023-07-20 | PXD029305 | Pride
Hcp profiler Protein quantification Data validation. Data-independent acquisition (dia) Host cell proteins (hcps) 
ProteomicsAdvantage of using scout-triggered multiple reaction monitoring mass spectrometry for robust quantification of host cell proteins in biotherapeutics matrices
The European and United States Pharmacopeias require the characterization and quantification of host cell proteins (HCPs) contaminating biological products, as these proteins pose a potential risk to clinical safety and product stability. The Enzyme-linked ImmunoSorbent Assay (ELISA) is currently th...
ORGANISM(S): Cricetulus griseus (Chinese hamster) (Cricetulus barabensis griseus) 
2026-02-23 | PXD066465 | Pride
Bioanalysis Biopharmaceutical characterization Host cell protein Analytical biochemistry Cho Chinese hamster ovary Hcp Antibody(s) 
ProteomicsA Sensitive, Rapid, Robust and Reproducible Workflow for Host Cell Protein Profiling in Biopharmaceutical Process Development
There is a growing industry and regulatory need to detect host cell protein (HCP) impurities in the production of protein biopharmaceuticals, as certain HCPs can impact product stability, safety, and efficacy, even at low levels.1-6 In some cases, regulatory agencies require the identification as we...
ORGANISM(S): Mus musculus (Mouse) 
2020-06-02 | PXD018427 | Pride
Monoclonal antibody (mab) Drug product (dp) Evosepone Drug substance (ds) Host cell proteins (hcps) Lc-ms/ms 
ProteomicsDevelopment of an optimized LC-MS workflow for Host Cell Protein characterization to support upstream process development
Host cell proteins (HCPs) co-expressed during the production of biotherapeutics can affect the safety, efficacy and stability of the final product. As such, monitoring HCPs populations and amounts throughout the production and purification process are an essential part of the overall quality control...
ORGANISM(S): Cricetulus griseus (Chinese hamster) (Cricetulus barabensis griseus) 
2025-05-07 | PXD053035 | Pride
Process analytical technologies Host cell proteins Lc-ms Clarified cell culture fluid Absolute quantification Chinese hamster ovary Data-independent acquisition Hi3 quantification Bio-processing 
ProteomicsLabel-free Quantification of Host-Cell Protein Impurity in a Recombinant Hemoglobin Reference Material
Quantitative analysis depends on pure-substance primary calibrators with known mass fractions of impurity. The datasets included were generated using mass spectrometry for the evaluation of label-free quantification (LFQ) as a method for determining the mass fraction of host-cell proteins (HCPs) in...
ORGANISM(S): Homo sapiens (Human) Escherichia coli Saccharomyces cerevisiae (Baker's yeast) 
2023-05-09 | PXD041736 | Pride
Protein reference materials Dda-pasef Label-free quantification Host-cell proteins 
ProteomicsQuantitative Host Cell Protein Analysis across Innovator Monoclonal Antibody-Based Protein Therapeutics using the Orbitrap Astral Mass Spectrometer
Protein biotherapeutics such as monoclonal antibodies (mAbs) are generally produced using cell-based production systems that generate endogenous proteins, commonly referred to as host cell proteins (HCPs), along with the desired drug products. A critical challenge is the removal of HCPs from the fin...
ORGANISM(S): Mus musculus (Mouse) Cricetulus griseus (Chinese hamster) (Cricetulus barabensis griseus) 
2026-04-20 | PXD067004 | Pride
Host cell proteins Mab based therapeutics Orbitrap astral ms Mass spectrometry 
ProteomicsAdvanced mass spectrometry workflows for accurate quantification of trace-level host cell proteins in drug products: benefits of FAIMS separation and gas-phase fractionation DIA
In this study, we investigated the benefits of adding high-field asymmetric ion mobility spectrometry (FAIMS) separation prior to data dependent acquisition (DDA) and gas phase fractionation (GPF) prior to data independent acquisition (DIA) LC-MS/MS analysis. Native digestion followed by LC-MS/MS wi...
ORGANISM(S): Mus musculus (Mouse) Cricetulus griseus (Chinese hamster) (Cricetulus barabensis griseus) 
2023-05-07 | PXD039582 | Pride
Data-independent analysis (dia) Host cell proteins (hcp) Final drug products (dp) 
ProteomicsAdvanced mass spectrometry workflows for accurate quantification of trace-level host cell proteins in drug products: benefits of FAIMS separation and gas-phase fractionation DIA
In this study, we investigated the benefits of adding high-field asymmetric ion mobility spectrometry (FAIMS) separation prior to data dependent acquisition (DDA) and gas phase fractionation (GPF) prior to data independent acquisition (DIA) LC-MS/MS analysis. Native digestion followed by LC-MS/MS wi...
ORGANISM(S): Mus musculus (Mouse) Cricetulus griseus (Chinese hamster) (Cricetulus barabensis griseus) 
2023-05-07 | PXD039585 | Pride
Faims Data-dependent analysis (dda) Host cell proteins (hcp) Final drug products (dp) 
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