The type VI secretion system (T6SS) is a bacterial contractile nanomachine that translocates toxic effectors into neighboring cells, helping bacteria to survive in competitive environments. The core T6SS structural protein Hcp forms the inner tube and mediates the loading and delivery of diverse eff...
Salmonella Entertidis (SE) causes persistent infections and egg contamination in laying ducks.Hcp roles as the core structural and effector proteins of T6SS. We generated an hcp deletion mutant MY1△hcp and detected its ability to invade duck granulosa cells (dGCs) and contaminate eggs. Then, Quantit...
We have created a CP-HCP method to characterise the carrier proteome of a host cell expression system producing recombinant protein biotherapeutic, which can be compared with the testing sample mixture containing trace amounts of host cell proteins against high-background recombinant protein and als...
Monitoring of host cell proteins (HCPs) during the manufacture of monoclonal antibodies (mAb) has become a critical requirement to provide effective and safe drug product. ELISA assays are still the current gold standard for the quantification of protein impurities. However, this technique has sever...
In order to examine how IOX1 affects the global Wnt target gene transcriptome in colorectal cancer cells, we performed RNA-sequencing in HCT116, DLD-1 and HCP-1 cells treated with IOX1. IOX1 led to global inhibition of Wnt target transcription in colorectal cancer cells.
HCP analysis of the various trypsins was performed using the X! Tandem search engine (Alanine) (http://www.thegpm.org). All data was searched against the downloaded UniProt Sus scrofa proteome (40.706 sequences, March 10, 2019) and the most recent cRAP list (http://www.thegpm.org). Data were search ...
