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ProteomicsHRD1 IP-MS in WT and SEL1L deficiency HEK293T cells
To gain further insights into the role and importance of SEL1L in HRD1 ERAD, we performed unbiased proteomics LC-MS analyses to map SEL1L and HRD1 interactomes in WT and SEL1L-/-, WT and HRD1-/- HEK293T cells, respectively.
ORGANISM(S): Homo sapiens (Human) 
2024-01-10 | PXD043674 | Pride
Erad complex formation Sel1l-hrd1 erad Hek293t 
ProteomicsProteomic screens of SEL1L-HRD1 ER-associated degradation substrates reveal its vital importance in the biogenesis of glycosylphosphatidylinositol-anchored proteins
The SEL1L-HRD1 protein complex of endoplasmic reticulum (ER)-associated degradation (ERAD) plays indispensable roles for many physiological processes in a substrate-specific manner; however, the nature of endogenous substrates remains largely elusive. Here we have developed a unique proteomics strat...
ORGANISM(S): Homo sapiens (Human) 
2023-12-17 | PXD041882 | Pride
Disease mutants Endogenous substrates Pigk Sel1l-hrd1 erad Proteomic screens Gpi-anchored proteins 
ProteomicsProteomic screens of SEL1L-HRD1 ER-associated degradation substrates reveal its vital importance in the biogenesis of glycosylphosphatidylinositol-anchored proteins
The SEL1L-HRD1 protein complex of endoplasmic reticulum (ER)-associated degradation (ERAD) plays indispensable roles for many physiological processes in a substrate-specific manner; however, the nature of endogenous substrates remains largely elusive. Here we have developed a unique proteomics strat...
ORGANISM(S): Homo sapiens (Human) Mus musculus (Mouse) 
2023-12-17 | PXD041803 | Pride
Endogenous substrates Pigk Disease mutants   Sel1l-hrd1 erad Proteomic screens Gpi-anchored proteins 
ProteomicsHrd1 interaction proteomics - Lipid disequilibrium disrupts ER proteostasis by impairing ERAD substrate glycan trimming and dislocation
Endoplasmic reticulum (ER)-associated degradation (ERAD) mediates the degradation of misfolded and unoligomerized proteins in the early secretory pathway. ERAD substrates are detected and delivered to membrane-embedded dislocation complexes. Following transfer into the cytosol, substrates are rapidl...
ORGANISM(S): Homo sapiens (Human) 
2017-01-03 | PXD005633 | Pride
Erad Hrd1 Triacsin c 
ProteomicsIdentification of interacting partners of Hrd1 in Arabidopsis
We performed a co-immunoprecipitation using anti-HRD1 antibodies on wild-type Arabidopsis lysates, followed by label-free quantitative mass spectrometry. Mock IPs (beads without antibody) were used to assess background binding.
ORGANISM(S): Arabidopsis thaliana (Mouse-ear cress) 
2025-09-29 | PXD065941 | Pride
Hrd1 Dia Arabidopsis Interaction 
GenomicsNext Generation Sequencing Facilitates Quantitative Analysis of PIGS-KO, PIGS-HRD1-DKO, PIGS-HRD1-CD55-TKO, and PIGS-HRD1-CD55-TKO+HA-CD55 Transcriptomes
Next Generation Sequencing Facilitates Quantitative Analysis of PIGS-KO, PIGS-HRD1-DKO, PIGS-HRD1-CD55-TKO, and PIGS-HRD1-CD55-TKO+HA-CD55 Transcriptomes
PRJNA766608 | ENA
ProteomicsRegulation of hepatic inclusions and fibrinogen biogenesis by SEL1L-HRD1 ERAD
Impaired secretion of an essential blood coagulation factor fibrinogen leads to hepatic fibrinogen storage disease (HFSD), characterized by the presence of fibrinogen-positive inclusion bodies and hypofibrinogenemia. However, the molecular mechanisms underlying the biogenesis of fibrinogen in the en...
ORGANISM(S): Mus musculus (Mouse) 
2024-09-11 | PXD047658 | Pride
Hepatocytes Sel1l-hrd1 erad Fibrinogen Inclusion bodies 
ProteomicsHRD1-ERAD controls production of the hepatokine FGF21 through CREBH polyubiquitination
As a ubiquitin ligase, HRD1 may regulate FGF21 expression through targeting protein degradation. We then aimed to identify proteins whose expressions are altered at post-transcriptional levels in HRD1 LKO mouse liver
ORGANISM(S): Mus Musculus 
2021-12-14 | PXD030410 |
ProteomicsHepatic Sel1L-Hrd1 ER-associated degradation deficiency leads to intrahepatic cholestasis in mice
The liver is the central organ critically regulating the balance of the metabolically potent yet toxic bile acids in the body. While genomic association studies have pointed to hepatic Sel1L – a critical component of mammalian Hrd1 ER-associated degradation (ERAD) machinery – as an influencer of ser...
ORGANISM(S): Mus musculus (Mouse) 
2023-03-11 | PXD035243 | Pride
Bile Liver Cholestasis Progressive familial intrahepatic cholestasis Abcb4 Sel1l-hrd1 erad 
TranscriptomicsThe ER-Associated Degradation Adapter Protein Sel1L Regulates Triglyceride Metabolism via Lipoprotein Lipase
Sel1L is an adaptor protein for the E3 ligase Hrd1 in the endoplasmic reticulum-associated degradation (ERAD), but its physiological role in a cell-type-specific manner remains unclear. Here we show that mice with adipocyte-specific Sel1L deficiency are resistant to diet-induced obesity and exhibit ...
ORGANISM(S): Mus musculus 
2015-05-18 | E-GEOD-56918 | biostudies-arrayexpress
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