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ProteomicsDirect analysis of IFITM3 S-fatty-acylation, part 2
Given our laboratory interest in IFITM3 S-fatty-acylation and antiviral activity, we sought to directly characterize fatty acids that are covalently attached to the Cys residues of IFITM3. IFITM3 comprises two S-fatty-acylation sites (C71 and C72) in proximity of a intramembrane domain, and anothe...
ORGANISM(S): Homo sapiens (Human) 
2018-03-28 | PXD008401 | Pride
Ifitm3 S-fatty-acylation Site identification S-palmitoylation 
ProteomicsDirect analysis of IFITM3 S-fatty-acylation, part 1
Given our laboratory interest in IFITM3 S-fatty-acylation and antiviral activity, we sought to directly characterize fatty acids that are covalently attached to the Cys residues of IFITM3. IFITM3 comprises two S-fatty-acylation sites (C71 and C72) in proximity of a intramembrane domain, and another ...
ORGANISM(S): Homo sapiens (Human) 
2018-03-28 | PXD008400 | Pride
Post translational modifications Modification site identification S-fatty-acylation Ifitm3 S-palmitoylation 
ProteomicsWild-type IFITM3 and mut 4KR-IFITM3 comparative interactome analysis in THP1
wild-type (WT) IFITM3 and 4KR-IFITM3 comparative interactome identified by Mass Spectrometry in THP1
ORGANISM(S): Homo sapiens (Human) 
2023-03-07 | PXD038896 | Pride
Human Ifitm3 Interactome 
ProteomicsNovel interacting proteins identified by tandem affinity purification and mass spectrometry associated with IFITM3 protein during PDCoV infection
To clarify novel roles of IFITM3 during PDCoV infection，host cell proteins that interact with IFITM3 were screened by tandem affinity purification coupled with mass spectrometry (TAP/MS) in ST cell line stably expressing IFITM3 via lentivirus.
ORGANISM(S): Sus Scrofa Domesticus 
2023-12-02 | PXD047480 |
ProteomicsIFITM3 restricts virus-induced inflammatory cytokine production by limiting Nogo-B mediated TLR responses
Interferon-induced transmembrane protein 3 (IFITM3) is a restriction factor that limits viral pathogenesis and exerts poorly understood immunoregulatory functions. Here, using human and mouse models, we demonstrate that IFITM3 promotes MyD88-dependent, TLR-mediated IL-6 production following exposure...
ORGANISM(S): Mus musculus (Mouse) 
2022-07-28 | PXD035254 | Pride
Viral pathogenesis Ip-mass spec Silac 
ProteomicsIFITM3 BioID Interactomes in B-ALL and MCL
To determine the roles of membrane associated IFITM3 in BCR-signalling in B-cell malignancies we expressed N-terminal BirA-IFITM3-Y20E fusion or BirA-EV control in patient derived B-ALL cells (PDX2) and Jeko1 Mantle cell lymphoma (MCL) cells. Additionally to examine the role of PIP3 binding residues...
ORGANISM(S): Homo sapiens (Human) 
2020-12-18 | PXD020697 | Pride
Human b-all mcl b-cells 
ProteomicsIFITM3 amino acid mutants phosphoproteomics
To investigate the role of IFITM3 in B-ALL, patient derived xenograft cells PDX2 were transduced with either a the constitutively membrane associated IFITM3-Y20E mutant or EV control. Additional mutation of PIP3 binding sites within the conserved internal loop region - K83/K104 or R85/R87/K88 - were...
ORGANISM(S): Homo sapiens (Human) 
2020-12-18 | PXD020696 | Pride
Pdx b-all b-cell 
ProteomicsA proteomic-based approach identifies the NEDD4-adaptor NDFIP2 as an important regulator of IFITM3
IFITMs are a family of highly related interferon-induced transmembrane proteins that interfere with the processes of fusion between viral and cellular membranes and are thus endowed with broad antiviral properties. A number of studies have shown how the antiviral potency of IFITMs is highly dependen...
ORGANISM(S): Homo sapiens (Human) 
2023-10-26 | PXD043581 | Pride
Ifitm3 Degradation Virus infection Vesicles Nanolc-ms/ms 
TranscriptomicsRNA-Seq of influenza A/Puerto Rico/8/1934 (H1N1) virus infected monoclonal cell lines with depressed IFITM3 gene activity
In this study monoclonal cell lines carrying mutations in IFITM3 gene were obtained based on WI-38 VA13 cells. To research the involvement of the IFITM3 gene in cellular response to Influenza A virus infection, original WI-38 VA13 cells and the clones with depressed IFITM3 gene activity (F3, F5, Е12...
ORGANISM(S): Homo sapiens 
2023-12-16 | E-MTAB-13582 | biostudies-arrayexpress
ProteomicsGenetic ablation of ifitm1 and ifitm3 attenuates IFN- mediated induction of ISG15 and MHC class I molecules
Interferon-induced transmembrane protein (IFITM1) plays a dual role in restriction of RNA viruses and in metastatic cancer cell growth. The signal transduction events that are orchestrated by IFITM1 are not well defined. We set out to identify IFITM1 interacting proteins to begin to define its mecha...
ORGANISM(S): Homo sapiens (Human) 
2020-05-26 | PXD007562 | Pride
Human Cancer Swath Silac Cell lines Ifn Lc-ms/ms 
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