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MetabolomicsGlobal 13C tracing and metabolic flux analysis of intact human liver tissue ex vivo
Liver metabolism is central to human physiology and influences the pathogenesis of common metabolic diseases. Yet, our understanding of human liver metabolism remains incomplete, with much of current knowledge based on animal or cell culture models that do not fully recapitulate human physiology. He...
2024-07-23 | MTBLS10481 | MetaboLights
ProteomicsHuman serum N-linked intact glycopeptides LC-MS/MS
Large-scale identification of N-linked intact glycopeptides by liquid chromatography coupled tandem mass spectrometry (LC-MS/MS) in human serum is challenging due to the wide dynamic range of serum protein abundances, the lack of a complete serum N-Glycan database and the existence of non-specifical...
ORGANISM(S): Armoracia rusticana Homo sapiens (Human) Gallus gallus (Chicken) 
2020-04-21 | PXD015622 | Pride
N-linked intact glycopeptide Co-elution Spectral library search Human serum Isotopic distribution 
ProteomicsGlobal quantification of intact proteins via chemical isotope labeling and mass spectrometry
Although thousands of intact proteins can be feasibly identified in recent years, global quantification of intact proteins is still challenging. Herein, we develop a high-throughput strategy for global intact protein quantification based on chemical isotope labeling. The isotope incorporation effici...
ORGANISM(S): Saccharomyces cerevisiae (Baker's yeast) 
2019-04-30 | PXD012526 | Pride
Intact protein quantification Ptop Chemical isotope labeling Highest isotopic peak Mass spectrometry 
TranscriptomicsSuv39h-dependent H3K9me3 represses intact retrotransposons in mouse embryonic stem cells
Heterochromatin is required to restrict aberrant expression of retrotransposons, but it remains poorly defined due to the underlying repeat-rich sequences. We dissected Suv39h-dependent histone H3 lysine 9 trimethylation (H3K9me3) by genome-wide ChIP-sequencing in mouse embryonic stem cells (ESCs). ...
ORGANISM(S): Mus musculus 
2014-06-26 | E-GEOD-57092 | biostudies-arrayexpress
ProteomicsGlycosylation Profiling of Neoplastic Biomarker Alpha Fetoprotein Through Intact Mass Protein Analysis
Glycosylation is an important post-translational modification (PTM) mechanism of proteins, that modulates protein function. Measurement of clinically adopted cancer protein biomarkers is commonly performed using affinity-based techniques, which often suffer from poor specificity and inability to dis...
ORGANISM(S): Homo sapiens (Human) 
2023-01-11 | PXD038602 | Pride
Intact proteins Hepatocellular carcinoma 
TranscriptomicsTU-tagging: cell type specific RNA isolation from intact complex tissues
We show that the combination of spatially restricted uracil phosphoribosyltransferase (UPRT) expression with 4-thiouracil (4TU) delivery can be used to label and purify cell type specific RNA from intact complex tissues. This method is useful for isolating RNA from cell types that are difficult to i...
ORGANISM(S): Drosophila melanogaster 
2010-06-19 | E-GEOD-15445 | biostudies-arrayexpress
ProteomicsIntact N-glycopeptides and proteomics from HCC tissue tumors with low and high AFP concentrations
Hepatocellular carcinoma (HCC) is still one of the malignant tumors with high morbidity and mortality in China and worldwide. Although AFP have been widely used as important biomarkers for HCC diagnosis and evaluation, the AFP level has a huge variation among HCC patient populations. Understanding t...
ORGANISM(S): Homo sapiens (Human) 
2020-03-19 | PXD016406 | Pride
Proteomics Intact glycopeptide Mass spectrometry Hepatocellular carcinoma 
TranscriptomicsIntact ureteric buds gene expression with AngII treatment
Compare the gene expression in intact Ubs after treated with AngII vs. Media, determine the key genes related to the ub branching gene expression change pattern. Two condition experiments, media and AngII. Biological replicate. Two for media samples, two for AngII treatment samples.
ORGANISM(S): Mus musculus 
2010-11-01 | E-GEOD-24932 | biostudies-arrayexpress
ProteomicsMulti-level – Intact, Subunits, and Peptides – Characterization of Antibody-Based Therapeutics by a Single-Column LC-MS setup
A comprehensive characterization of biotherapeutics, mandated by regulatory authorities, require analyses of a protein drug at multiple structure levels. Such multi-level characterization can be performed by mass spectrometry (MS), with established conventional MS-based assays of product quality att...
ORGANISM(S): Cricetulus griseus (Chinese hamster) (Cricetulus barabensis griseus) 
2025-02-26 | PXD054948 | Pride
Subunits Intact Lc-ms Ead C4-column Peptides 
ProteomicsWavelength Dependence of Intact Protein Extraction Using Femtosecond Laser Ablation
Raw files for the paper named: Wavelength Dependence of Intact Protein Extraction Using Femtosecond Laser Ablation
ORGANISM(S): Mastigocladopsis Repens Rhodopsin Equine Myoglobin Lysozyme From Chicken Egg White 
2025-03-04 | MSV000097234 | MassIVE
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