Sort   by:  
 Page size 
MetabolomicsA combined direct infusion/reversed phase chromatography – high resolution mass spectrometry workflow for accurate absolute lipid quantification with 13C- internal standards
We propose a fully automated novel workflow for lipidomics based on flow injection, followed by liquid chromatography-high-resolution mass spectrometry (FI/LC-HRMS). The workflow combined in-depth characterization of the lipidome achieved via reversed-phase LC-HRMS with absolute quantification by us...
2021-07-01 | MTBLS1876 | MetaboLights
ProteomicsLabel-Free Quantification of Yeast Cellular Proteome
Precursor ion intensity quantification and intensity-based absolute quantity estimation (IBAQ) was used to on the mixture of yeast cell lysates in order to estimate the absolute concentrations of the proteins.
ORGANISM(S): Saccharomyces cerevisiae (Baker's yeast) 
2020-03-06 | PXD012803 | Pride
Lc-ms Intensity-based absolute quantification Saccharomyces cerevisiae Label-free quantification 
ProteomicsTMT and Label-Free Quantification of Yeast Mitochondrial and Cellular Proteome During Different Stages of Growth
Tandem mass tag (TMT)-based relative quantification was used in combination with the intensity-based absolute quantity estimation (IBAQ) to study of both yeast cells and isolated yeast mitochondria during fermentative growth, diauxic shift, respiratory growth.
ORGANISM(S): Saccharomyces cerevisiae (Baker's yeast) 
2020-03-06 | PXD012802 | Pride
Lc-ms Intensity-based absolute quantification Saccharomyces cerevisiae Label-free quantification Tandem mass tag Diauxic shift 
ProteomicsIntensity-based absolute quantification (iBAQ) of proteins in MDA-MB231
To determine the absolute copy number of proteins in MDA-MB231 breast cancer cells, we employed IBAQ mediated absolute quantification of proteins based on (Schwanhäusser et al., Nature, 2011), with some modifications. Maqquant calculated iBAQ values were calibrated using spike-in standards, and used...
ORGANISM(S): Homo sapiens (Human) 
2021-05-14 | PXD024139 | Pride
Ibaq  absolute protein quantification  breast cancer  mda-mb231 
ProteomicsAbsolute quantification of proteins and histone modifications in Drosophila embryos reveals stoichiometric relationships in chromatin regulation
Gene transcription in eukaryotes is regulated through dynamic interactions of a variety of different proteins with DNA in the context of chromatin. Here, we used mass spectrometry for absolute quantification of the nuclear proteome during two stages of Drosophila embryogenesis. Before proteomics ana...
ORGANISM(S): Drosophila melanogaster (Fruit fly) 
2023-02-01 | PXD015214 | Pride
Absolute proteome quantification/ histone modification quantification Prc2 H3k27 methylation Drosophila 
TranscriptomicsMicroarray probe design
MicroRNAs (miRNAs) have been shown to play an important role in many different cellular, developmental, and physiological processes. Accordingly, numerous methods have been established to identify and quantify miRNAs. The shortness of miRNA sequence results in a high dynamic range of melting tempera...
ORGANISM(S): synthetic construct 
2009-10-26 | E-GEOD-15832 | biostudies-arrayexpress
ProteomicsMembrane modulation of super-secreting “midiBacillus” expressing the major Staphylococcus aureus antigen – a mass-spectrometry based absolute quantification approach.
Bacillus subtilis is a Gram-positive bacterium considered as a “cell-factory” for industrial enzymes and biopharmaceuticals. Given the commercial advantage of this organism, researchers have been working towards the improvement of this organism as a producer of secreted proteins. Nonetheless, there ...
ORGANISM(S): Bacillus subtilis subsp. subtilis 
2020-03-20 | PXD015496 | Pride
Srm Membrane proteins Heterologous protein production Proteomics Absolute quantification 
ProteomicsComprehensive Absolute Quantification of the Cytosolic Proteome of Bacillus subtilis by Data Independent, Parallel Fragmentation in Liquid Chromatography/Mass Spectrometry (LC/MS)
Absolute protein quantification for cytosolic proteins of B. subtilis growing on a carbon source (condition S) or on an amino acid source (condition CH). Data for 3 biological replicates and 3 technical replicates each for each condition are provided. For data processing and protein identification, ...
ORGANISM(S): Bacillus subtilis subsp. subtilis str. 168 
2014-08-04 | PXD000154 | Pride
Absolute protein quantification Mse Synapt (waters) Dia B. subtilis 
ProteomicsDeconvoluting the complexity of Bacillus subtilis disulfide stress responses by redox-state and absolute abundance quantification of extracellular, membrane and cytosolic proteins
To understand cellular mechanisms of stress management, omics data constitute a powerful resource. However, the availability of absolute quantitative data on protein abundances is still a serious limiting factor. This knowledge gap increments especially when protein abundances need to be compared ac...
ORGANISM(S): Bacillus subtilis subsp. subtilis str. 168 
2024-02-05 | PXD042394 | Pride
Absolute protein quantification / bacillus subtilis / disulfide stress / redox proteomics / subcellular fractionation 
ProteomicsPeptide Filtering and Quantification Methods
In bottom-up proteomics, data are acquired on peptides resulting from proteolysis. In XIC-based quantification, the quality of the protein abundance estimation depends on how peptide data are filtered and on which quantification method is used to sum up peptide intensities into protein abundances. S...
ORGANISM(S): Saccharomyces cerevisiae (Baker's yeast) 
2018-11-14 | PXD009740 | Pride
Peptide filtering Quantification methods Extracted ion current 
Sort   by:  
 Page size