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Chromatin proteins mediate replication, regulate expression and ensure integrity of the genome. So far, a comprehensive inventory of interphase chromatin has not been determined. This is largely due to its heterogeneous and dynamic composition, which makes conclusive biochemical purification difficu...
ORGANISM(S): Homo sapiens (Human) 
2014-02-18 | PXD000493 | Pride
Purpose: to identify how condensin I removal affects gene expression globally. Methods: Chicken DT40 CAP-H KO cells were treated with or without dox for 36 h. Total RNA samples were extracted and subjected to sequencing using an Illumina Hiseq2000 platform. Library preparation and Illumina sequencin...
ORGANISM(S): Gallus gallus 
In higher eukaryotes centromeres often coalesce into a large intranuclear domain called the chromocenter. Chromocenters are important for the organization of pericentric heterochromatin and a disturbance of their formation results in an upregulation of repetitive elements and causes defects in chrom...
ORGANISM(S): Drosophila melanogaster (Fruit fly) 
2020-03-23 | PXD012551 | Pride
Here we compare the distribution of insulator proteins during interphase and mitosis. We performed ChIP-seq analysis on purified populations of interphase and mitotic Kc cells, using antibodies against CP190, dCTCF, BEAF, and Su(Hw). Examination of 4 different insulator proteins during interphase a...
ORGANISM(S): Drosophila melanogaster 
Differential nucleosome organization in human interphase and metaphase chromosomes [ChemMap]
Across the cell cycle, mitochondrial dynamics are regulated by a cycling wave of actin polymerization/depolymerization. In metaphase, this wave induces the assembly of actin comet tails on mitochondria that propel these organelles to drive spatial mixing,resulting in their equitable inheritance by d...
ORGANISM(S): Homo sapiens (Human) 
2024-06-23 | PXD050085 | Pride
Differential nucleosome organization in human interphase and metaphase chromosomes [MNase-Seq]
Differential nucleosome organization in human interphase and metaphase chromosomes [Mnase-seq]
Differential nucleosome organization in human interphase and metaphase chromosomes [Chemical Mapping]
Mixed Lineage Leukemia (MLL) and its metazoan Trithorax orthologs have been linked with the epigenetic maintenance of transcriptional activity. To identify mechanisms by which MLL perpetuates active transcription in dividing cells, we investigated its role during M-phase of the cell cycle. Unlike ot...
ORGANISM(S): Homo sapiens 
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