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TranscriptomicsDual IRE1 RNase functions dictate glioblastoma development
The transcriptome dataset has been used to validate on a GBM patient cohort (n=117) the IRE1-dependent gene expression signature identified in U87 cell line.
ORGANISM(S): Homo sapiens 
2018-01-09 | E-MTAB-6326 | biostudies-arrayexpress
TranscriptomicsGene profiling: U87 IRE1 dominant negative cells vs. U87ctrl cells in culture
Transcriptome analysis was performed from human U87 glioblastoma cell clones: U87 IRE1.NCK DN (U87dn, IRE1 dominant negative) and U87 control (U87ctrl, empty plasmid). Cells were grown in DMEM supplemented with 10% FBS and glutamine for 16 hours in culture prior mRNA isolation and analyses U87dn c...
ORGANISM(S): Homo sapiens 
2010-08-08 | E-GEOD-22385 | biostudies-arrayexpress
ProteomicsA novel blood brain barrier-permeable IRE1 inhibitor prevents Glioblastoma relapse in mice
Inositol Requiring Enzyme 1 (IRE1) is a bifunctional serine/threonine kinase and endoribonuclease that is a major mediator of the Unfolded Protein Response (UPR) during endoplasmic reticulum (ER) stress. Tumor cells experience ER stress due to adverse environmental cues such as hypoxia or nutrient s...
ORGANISM(S): Homo sapiens (Human) 
2023-07-20 | PXD026908 | Pride
Ire1 Glioblastoma Unfolded protein response Inhibitors Endoplasmic reticulum 
TranscriptomicsUnfolded protein response in wildtype, ire1, gcn4, gcn2
We measured steady-state mRNA levels by microarray hybridization, comparing WT, (delta)ire1, (delta)gcn4, and (delta)gcn2 cells treated with 2 mM DTT for 30 min (by which time the UPR is qualitatively complete) to untreated samples of the same genotype. WT cells were taken as a positive control for ...
ORGANISM(S): Saccharomyces cerevisiae 
2010-06-06 | E-GEOD-1688 | biostudies-arrayexpress
ProteomicsThe ER stress sensor IRE1 interacts with STIM1 to promote store-operated calcium entry, T cell activation, and muscular differentiation
A quantitative phosphoproteomic study was performed on mouse embryonic fibroblasts (MEF) knocked-out of IRE1 protein and re rexpressin it culturen in 25mM glucose media. The chosen experimental strategy was to perform phosphopeptide enrichment associated with multiplex protein identification and qua...
ORGANISM(S): Mus musculus (Mouse) 
2023-12-08 | PXD046579 | Pride
Ire1 Soce Er stress Stim1 
ProteomicsIRE1-dependent GOLIM4 expression controls protein secretion to modulate glioblastoma cell adhesion and migration.
. In the present study, we investigated if and how IRE1 could regulate the functionality of the secretory machinery in GB cells and identified GOLIM4, a Golgi-associated molecule whose expression is regulated downstream of IRE1 through the transcription of XBP1s.
ORGANISM(S): Homo sapiens (Human) 
2026-03-02 | PXD074925 | Pride
Ire1 Golim4 Glioblastoma Secretome 
ProteomicsIdentification of autophosphorylationsites in recombinant Saccharomyces cerevisiae Ire1
This study reports autophosphorylation sites in the serine/threonine protein kinase Ire1. The cytosolic portion of Ire1 was expressed as an glutathione S transferase fusion protein in Escherichia coli. Phosphroyaltion sites in the recombinant wild type protein and a L745A mutant were mapped by mass ...
ORGANISM(S): Saccharomyces cerevisiae (Baker's yeast) 
2017-06-09 | PXD004924 | Pride
Ire1 Yeast Phosphorylation Protein kinase Unfolded protein response 
TranscriptomicsTranscriptome analysis of ire1 mutants after treatment with or without tunicamycin in the presence of actinomycin D
Arabidopsis seedlings of wildtype or ire1a ire1b double mutant were treated with or without tunicamycine in the presence of actinomycin D (ActD). 12 samples
ORGANISM(S): Arabidopsis thaliana 
2013-03-19 | E-GEOD-39690 | biostudies-arrayexpress
TranscriptomicsIRE1-dependent transcriptome remodelling upon ER stress in human glioma cells
We investigate the contribution of IRE1 signaling to the modulation of U87 glioma cells transcriptome upon various stresses. To this end, IRE1 control and IRE1 dominant negative expressing U87 glioma cells were subjected to environmental or chemical challenges and their transcriptome monitored using...
ORGANISM(S): Homo sapiens 
2011-12-31 | E-GEOD-27306 | biostudies-arrayexpress
GenomicsIRE1 inhibitor [RNA-seq]
IRE1 inhibitor [RNA-seq]
PRJNA949269 | ENA
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