Sort   by:  
 Page size 
TranscriptomicsSaccharomyces cerevisiae Transcript Isoform mapping
Extensive transcriptional heterogeneity revealed by isoform profiling Application of TIF-Seq (Transcript IsoForm Sequencing) to S.cerevisiae. The method was applied to simultaneously identify the 5' capped mRNA transcription start site and the 3' polyadenylation site in different conditions: WT cell...
ORGANISM(S): Saccharomyces cerevisiae 
2013-05-06 | E-GEOD-39128 | biostudies-arrayexpress
ModelsBoehm2014 - isoform-specific dimerization of pSTAT5A and pSTAT5B
Boehm2014 - isoform-specific dimerization of pSTAT5A and pSTAT5B To study STAT5 activation, the authors build a dynamic model of pSTAT5 isoform dimerization. Combinatorial binding of pSTAT5A and pSTAT5B is analysed using model hypotheses and concurrent experiments. Model parameters are derived from ...
2024-09-02 | BIOMD0000000591 | BioModels
TranscriptomicsIdentification of Common Genetic Variants that Account for Transcript Isoform Variation between Human Populations
In addition to the differences between populations in transcriptional and translational regulation of genes, alternative pre-mRNA splicing (AS) is also likely to play an important role in regulating gene expression and generating variation in mRNA and protein isoforms. Recently, the genetic contribu...
ORGANISM(S): Homo sapiens 
2010-08-19 | E-GEOD-9703 | biostudies-arrayexpress
TranscriptomicsIdentification of transcripts regulated by CEBPA protein and its P30 isoform in K562
To investigate the effect of CEBPA and its mutant isoform P30 on the expression of mRNAs and long non-coding RNAs (lncRNAs), we utilized the K562 AML cell line carrying a stable and Tet-on inducible CEBPA or P30 allele. Based on the expression of known CEBPA transcriptional targets, we selected RNA...
ORGANISM(S): Homo sapiens 
2015-06-10 | E-GEOD-65235 | biostudies-arrayexpress
ProteomicsSEPepQuant enhances the detection of possible isoform regulations in shotgun proteomics
Shotgun proteomics is essential for protein identification and quantification in biomedical research, but protein isoform characterization is challenging due to the extensive number of peptides shared across proteins, hindering our understanding of protein isoform regulation and their roles in norma...
ORGANISM(S): Homo sapiens (Human) 
2023-10-24 | PXD044809 | Pride
Protein isoform Shotgun proteomics Quantification 
ProteomicsIdentification of alternative splice variants using unique isoform-specific tryptic peptide sequences for database searches
Alternative splicing is a mechanism in eukaryotes by which different forms of messenger RNAs (mRNAs) are generated from the same gene. Identification of alternative splice variants requires the identification of peptides specific for alternative splice forms. For this purpose, we generated a human d...
ORGANISM(S): Homo sapiens (Human) 
2017-05-19 | PXD006026 | Pride
Protein species Proteomics Alternative splicing Mass spectrometry Phosphorylation Proteoform Isoforms 
TranscriptomicsIdentification of transcripts whose abundance changes in response to acute switches in DSX isoform
RNAseq of dissected adult fat body after DSX isoform is switched using temperature inducible alleles or constructs. Biological duplicates were sequenced for each genotype at each condition.
ORGANISM(S): Drosophila melanogaster 
2014-10-30 | E-GEOD-55848 | biostudies-arrayexpress
TranscriptomicsIdentification of Murine Uterine Genes Regulated in a Ligand-Dependent Manner by the Progesterone Receptor.
Progesterone (P4) acting through its cognate receptor, the progesterone receptor (PR), plays an important role in uterine physiology. The PR knockout (PRKO) mouse has demonstrated the importance of the P4-PR axis in the regulation of uterine function. To define the molecular pathways regulated by P4...
ORGANISM(S): Mus musculus 
2012-08-06 | E-GEOD-39920 | biostudies-arrayexpress
TranscriptomicsIsoform-specific transcriptional activity of overlapping targets that respond to thyroid hormone receptors a1 and b1
Thyroid hormone receptors (TRs) are hormone-regulated transcription factors that control multiple aspects of physiology and development. TRs are expressed in vertebrates as a series of distinct isoforms that exert distinct biological roles. We wished to determine if the two most widely expressed i...
ORGANISM(S): Homo sapiens 
2009-08-08 | E-GEOD-17561 | biostudies-arrayexpress
TranscriptomicsA putative network of actin-associated cytoskeletal proteins in glomerular podocytes defined by co-purified mRNAs
Identification of a putative network of actin-associated cytoskeletal proteins in glomerular podocytes defined by co-purified mRNAs. An experimental purification in which a presumed RNA binding protein was used for an RNA immunoprecipitation and microarray analysis (RIP-CHIP). Total cell extracts w...
ORGANISM(S): Mus musculus 
2009-08-01 | E-GEOD-16942 | biostudies-arrayexpress
Sort   by:  
 Page size