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ProteomicsAnalysis of pummelo proteome using iTRAQ
For protein identification, the following parameters were used. Peptide mass tolerance = 20 ppm, Missed cleavage = 2, MS/MS tolerance = 0.1 Da, Enzyme = Trypsin, Fixed modification: Carbamidomethyl (C), iTRAQ8plex(K), iTRAQ8plex(N-term), Variable modification：Oxidation(M)，Decoy database pattern=Reve...
ORGANISM(S): Citrus maxima (Pomelo) (Citrus grandis) 
2020-01-27 | PXD000527 | Pride
Itraq floral bud 
ProteomicsPorcine intestinal epithelial cells
Porcine deltacoronavirus (PDCoV) is an emerging pathogen of swine belonging to family Coronaviridae, genus Deltacoronavirus. PDCoV predominantly infects the porcine intestinal epithelial cells (IPECs) causing severe diarrhea and/or vomiting, dehydration, and death in piglets. However, there are no r...
ORGANISM(S): Sus scrofa domesticus (domestic pig) 
2020-10-19 | PXD019975 | Pride
Porcine intestine lc-ms/ms 
ProteomicsProteomic analysis of glutamine deprivation in HepG2 cells
We applied the quantitative proteomics approach to estimate the relative abundance of proteins in HepG2 cells treated by glutamine deprivation. Two independent cultured cell lysates, namely control and glutamine deprivation, were marked with iTRAQ labels and subjected to LC-ESI-MS/MS analysis. The t...
ORGANISM(S): Homo sapiens (Human) 
2016-02-09 | PXD003387 | Pride
Hepg2 cells Glutamine 
ProteomicsQuantitative proteomics of cytokine-induced memory-like (CIML) NK cells
NK cells from healthy donors were isolated from peripheral blood and stimulated overnight with IL-12/15/18 to induce a memory-like phenotype. After 7 days of culture, the phenotype and degranulation potential of CIML NK cells was characterized in two distinct NK cell subsets: CD16+/CD56+ and CD16−/C...
ORGANISM(S): Homo sapiens (Human) 
2025-07-14 | PXD059190 | Pride
Lc-msms Cd16+cd56+ Itraq8plex isobaric labeling Quantitative proteomics Ciml nk cells Cd16−cd56+ 
ProteomicsCandidate proteins involved in the calyx abscission process of ‘Kuerlexiangli’ (Pyrus sinkiangensis Yu) identified by iTRAQ analysis
‘Kuerlexiangli’ (Pyrus sinkiangensis Yu) is an important market pear in China. The shape and quality of the fruit is negatively affected by the presence of a persistent calyx. Here, to explore the molecular mechanism of calyx abscission, we designed an experiment to compare protein expression at two...
ORGANISM(S): Pyrus x bretschneideri 
2020-06-17 | PXD012499 | Pride
Itraq Pear Calyx abscission Differentially abundant proteins 
ProteomicsiTRAQ-based quantitative serum proteomic analysis in ischemic stroke patients
Hemorrhagic transformation (HT), which occurs with or without reperfusion treatments (thrombolysis and/or thrombectomy), deteriorates the outcomes of ischemic stroke patients. It is essential to find clinically reliable biomarkers that can predict HT. In this study, we screened for potential serum b...
ORGANISM(S): Homo sapiens (Human) 
2021-10-12 | PXD026950 | Pride
Hemorrhagic transformation Itraq Serum proteomics Acute ischemic stroke 
ProteomicsChanges in the ascending aorta proteome of cholesterol-fed rabbits
Here we undertook a proteomic investigation of ascending aorta from New Zealand White rabbits after 10 weeks on a high (2% w/w) cholesterol diet (HCD, n=5) or control diet (n=5) in order to profile the proteomic changes in response to the HCD. Histology confirmed intimal thickening in the HCD group ...
ORGANISM(S): Oryctolagus cuniculus (Rabbit) 
2018-06-21 | PXD002345 | Pride
Ascending aorta rabbit cholesterol itraq atherosclerosis 
ProteomicsiTRAQ-based quantitative proteomics analysis of human HNSCC cells
Head and neck squamous cell carcinoma (HNSCC) is the sixth most common cancer and is major cause of cancer mortality and morbidity. It emerges within oral cavity, lip, tongue, floor of the mouth, nasopharynx, palate, gingival and larynx, is common cancer worldwide especially in Southeast Asia and So...
ORGANISM(S): Homo Sapiens (ncbitaxon:9606) 
2016-03-14 | MSV000079580 | MassIVE
ProteomicsAnalysis of protein regulation in cucumber leaves after Corynespora cassiicola inoculation: a proteomic approach
Corynespora leaf spot (CSL), caused by Corynespora cassiicola, has become one of the most important foliar diseases of cultivated cucumber. However, the defense mechanisms of cucumber plants in response to C. cassiicola are still poorly understood. Here, proteins from resistant plants were analyzed ...
ORGANISM(S): Cucumis sativus 
2019-09-26 | PXD012903 | Pride
Corynespora cassiicola Histochemical staining Defense-related proteins Cucumber Itraq Qrt-pcr 
ProteomicsAn Isocaloric High-Fat Diet Improves Lifespan in Rats and Drosophila
The health effect of dietary fat has been one of the most vexing issues in the nutrition field. Few animal studies have examined the impact of high-fat diets on lifespan by controlling energy intake. In this study, we found that compared to a normal diet, an isocaloric high-fat diet (IHF) significan...
ORGANISM(S): Rattus norvegicus (Rat) 
2021-01-06 | PXD022585 | Pride
Isocaloric High-fat diet Proteomics Lifespan 
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