Sort   by:  
 Page size 
ProteomicsCX-MS of TFIIH and Def1 complex
The DNA damage response is an essential process for the survival of living cells. In a subset of stress-responsive genes in humans,Elongin controls transcription in response to multiple stimuli, such as DNA damage, oxidative stress, and heat shock. Yeast Elongin (Ela1-Elc1), along with Def1, is know...
ORGANISM(S): Saccharomyces cerevisiae (Baker's yeast) 
2018-10-22 | PXD008223 | Pride
Stress response Transcription Rna polymerase ii Tfiih Def1 
ProteomicsMass spectrometry study of formaldehyde cross-linking of structured proteins
Formaldehyde is a widely used fixative in biology and medicine. The current chemical model for formaldehyde cross-linking of proteins is the formation of a methylene bridge that incorporates one carbon atom into the link. Here, we present mass spectrometry data that largely refute this model. Instea...
ORGANISM(S): Homo sapiens (Human) 
2020-06-25 | PXD015435 | Pride
Xl-ms Nascent polypeptide-associated complex Actin Structural biology Cross-linking coupled to mass spectrometry 
ProteomicsFacilitating in situ cross-linking and mass spectrometry by antibody-based protein enrichment
Cross-linking of living cells followed by mass spectrometry identification of cross-linked peptides (in situ CLMS) is an emerging technology to study protein structures in their native environment. One of the inherent difficulties of this approach is the high complexity of the samples following cell...
ORGANISM(S): Homo sapiens (Human) 
2022-06-09 | PXD025099 | Pride
Cl-ms Antibody 
ProteomicsTargeted in situ cross-linking for determination of structure and interactions of SARS-CoV-2 proteins in the cellular context
Discovery of host-pathogen intra-cellular interactions is critical for identification of mechanisms of infection and discovery. Affinity purification based on Mass Spectrometry (AP-MS) approaches can identify the host-pathogen protein-protein interaction network that includes hundreds of cellular pr...
ORGANISM(S): Homo sapiens (Human) 
2021-10-06 | PXD023542 | Pride
Ap-ms Mass-spectrometry Sars-cov-2 Hek293 Nucleocapsid protein Lc-msms Nsp1 Strep-tag Nsp2 
ProteomicsTargeted in situ cross-linking for determination of structure and interactions of SARS-CoV-2 proteins in the cellular context
Cross-linking mass spectrometry (XL-MS) has progressed from studying purified protein assemblies in-vitro towards investigating the same assemblies in intact cells, tissues, and even whole organisms (so called “in situ XL-MS”). In situ XL-MS offers the great advantage of reporting on the architectur...
ORGANISM(S): Homo sapiens (Human) 
2021-10-06 | PXD023487 | Pride
Mass-spectrometry Xlms Sars-cov-2 Clms Hek293 Nucleocapsid protein Lc-msms Nsp1 Nsp2 
ProteomicsOpen search strategy for inferring the masses of cross-link adducts
Development of new reagents for protein cross-linking is continually ongoing. The chemical formulas of the linker adducts formed by these reagents are usually deduced from expert knowledge, and then validated by mass spectrometry. Clearly, it would have been more rigorous if the adduct masses are ...
ORGANISM(S): Bos taurus (Bovine) 
2021-09-09 | PXD020704 | Pride
Modifications Xl-ms Emcs Open search Cross-linking Mass spectrometry Glutaraldehyde 
Sort   by:  
 Page size