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ProteomicsStructure of TFIIH/Rad4-Rad23-Rad33 in damaged DNA opening in Nucleotide Excision Repair
TFIIH is a 10-protein complex that is conserved throughout eukaryotes. TFIIH has two primary cellular functions: transcription initiation and nucleotide excision repair (NER). NER in eukaryotes begins by recognition of a bulky lesion by the obligate dimer Rad4-Rad23. This is followed closely by the ...
ORGANISM(S): Saccharomyces cerevisiae (Baker's yeast) 
2021-07-07 | PXD021212 | Pride
Dna repair Tfiih Rad4 Rad33 Rad23 Dna damage Nucleotide excision repair 
TranscriptomicsTranscription profiling of S. cerevisiae TFIIH mutants treated with methyl methanesulfonate
Purpose of profiling experiment was to examine effects of a mutation of a subunit of TFIIH, ssl1p, which has ubiquitin ligase activity.,The whole complex has previously been shown to have both kinase and helicase activities. We wanted to see if 1) there were,general transcriptional defects with the...
ORGANISM(S): Saccharomyces cerevisiae 
2007-07-08 | E-GEOD-2343 | biostudies-arrayexpress
ProteomicsCX-MS of TFIIH and Def1 complex
The DNA damage response is an essential process for the survival of living cells. In a subset of stress-responsive genes in humans,Elongin controls transcription in response to multiple stimuli, such as DNA damage, oxidative stress, and heat shock. Yeast Elongin (Ela1-Elc1), along with Def1, is know...
ORGANISM(S): Saccharomyces cerevisiae (Baker's yeast) 
2018-10-22 | PXD008223 | Pride
Stress response Transcription Rna polymerase ii Tfiih Def1 
TranscriptomicsStably-paused genes revealed through inhibition of transcription initiation by the TFIIH inhibitor Triptolide
Transcription by RNA Polymerase II (Pol II) in metazoan is regulated in several steps, including preinitiation complex (PIC) formation, initiation, Pol II escape, productive elongation, cotranscriptional RNA-processing and termination. Genome-wide studies have demonstrated that the phenomenon of ...
ORGANISM(S): Homo sapiens 
2015-01-05 | E-GEOD-63314 | biostudies-arrayexpress
ProteomicsStructural basis of TFIIH activation for nucleotide excision repair
Nucleotide excision repair (NER) is the major DNA repair pathway that removes UV-induced and bulky DNA lesions. There is currently no structure of NER intermediates, which form around the large multisubunit transcription factor IIH (TFIIH). Here we report the cryo-EM structure of an NER intermediate...
ORGANISM(S): Homo sapiens (Human) 
2019-07-02 | PXD013947 | Pride
Ner pathway Bs3 cross-linking 
ProteomicsC. elegans TFIIH subunit GTF-2H5/TTDA is a non-essential transcription factor indispensable for DNA repairC. elegans TFIIH subunit GTF-2H5/TTDA is a non-essential transcription factor indispensable for DNA repair.
The 10-subunit TFIIH complex is vital to transcription and nucleotide excision repair. Hereditary mutations in its smallest subunit, TTDA/GTF2H5, cause a photosensitive form of the rare developmental disorder trichothiodystrophy. Some trichothiodystrophy features are thought to be caused by subtle t...
ORGANISM(S): Caenorhabditis elegans 
2021-11-19 | PXD029008 | Pride
Transcription C. elegans Ip-ms Dna repair Tfiih 
ProteomicsSystematic mutagenesis of TFIIH subunit p52/Tfb2 identifies residues required for XPB/Ssl2 subunit function and genetic interactions with TFB6
TFIIH is an evolutionarily conserved complex that plays central roles in both RNA polymerase II (pol II) transcription and DNA repair. As an integral component of the pol II Pre-Initiation Complex (PIC), TFIIH regulates pol II enzyme activity in numerous ways. The TFIIH subunit XPB/Ssl2 is an ATP-de...
ORGANISM(S): Homo sapiens (Human) 
2022-09-07 | PXD036107 | Pride
Tfiih p52/tfb2 xpb/ssl2 tfb6 
ProteomicsSystematic mutagenesis identifies p52/Tfb2 residues required for XPB/Ssl2 function within TFIIH and genetic interactions with TFB6
TFIIH is an evolutionarily conserved complex that plays central roles in both RNA polymerase II (pol II) transcription and DNA repair. As an integral component of the pol II Pre-Initiation Complex (PIC), TFIIH regulates the activity of the pol II enzyme in numerous ways. The TFIIH subunit XPB/Ssl2 i...
ORGANISM(S): Saccharomyces cerevisiae (Baker's yeast) 
2023-03-11 | PXD035576 | Pride
Mutagenesis Integrity Transcription start site Function P52/tfb2 Structure Rna polymerase ii transcription Gtf2h4 General transcription factor tfiih Tfb6 Xpb/ssl2 
GenomicsGenome binding profile of TFIIH helicases XPB and XPD in HT1080 cells
The conserved TFIIH helicases XPB and XPD play key roles in transcription initiation and DNA repair. To investigate the functions of these helicases on a genome-wide scale, we performed ChIP-seq of endogenous XPB and XPD in the HT1080 human fibrosarcoma cell line. 2 ChIP samples and 1 unenriched inp...
ORGANISM(S): Homo sapiens 
2014-02-18 | E-GEOD-44849 | biostudies-arrayexpress
UnknownThe essential and multifunctional TFIIH complex.
Not available
S-EPMC5980561 | biostudies-literature
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