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ProteomicsLow-input proteomics using lauryl maltose neopentyl glycol
We developed a sample preparation method for low-input proteomics using lauryl maltose neopentyl glycol. Using the developed method, we performed proteome analysis of coIP samples.
ORGANISM(S): Homo Sapiens (human) 
2024-03-06 | PXD048100 | JPOST Repository
ProteomicsLow-input proteomics using lauryl maltose neopentyl glycol
We developed a sample preparation method for low-input proteomics using lauryl maltose neopentyl glycol. Using the developed method, we performed proteome analysis of 100ng of HEK293T proteins, coIP samples, and serum extracellular vesicles, and further challenged label-free single-cell proteomics o...
ORGANISM(S): Homo Sapiens (human) Bos Taurus 
2024-03-06 | PXD044605 | JPOST Repository
ProteomicsValidation of Thin-diaPASEF for maximizing proteome coverage
We optimized the parameters of CCS range, polygon scan region, ramp time, and isolation window width to achieve high-depth proteome analysis using the timsTOF HT. As a result, we developed Thin-diaPASEF, which was optimized with a CCS range of 0.7–1.3, a ramp time of 150 ms, an isolation window widt...
ORGANISM(S): Homo Sapiens (human) Cellular Organisms 
2025-08-10 | PXD061613 | JPOST Repository
ProteomicsDevelopment of non-targeted proteome analysis of dried blood spots using an automated protein extraction system (
In this study, we developed a simple protein extraction method of dried blood spots (DBS) that potentially meets the throughput required for newborn screening (NBS) and optimized non-targeted proteomic analysis using this method in combination with liquid chromatography coupled mass spectrometry in ...
ORGANISM(S): Homo Sapiens (human) 
2025-08-14 | PXD064701 | JPOST Repository
ProteomicsProteome analysis of serum proteins enriched by lectins
Serum proteins enriched by 37 different lectins were analyzed by LC-MS/MS. It was found that the enrichment process with Solanum tuberosum lectin (STL) and Lycopersicon esculentum lectin (LEL) detected many trace proteins in the serum. In addition, combinations of lectins were examined, and the comb...
ORGANISM(S): Homo Sapiens (human) Gallus Gallus Rattus Norvegicus Ovis Aries Cellular Organisms Capra Hircus Bos Taurus Mus Musculus (mouse) 
2024-02-23 | PXD047848 | JPOST Repository
ProteomicsProteome analysis of serum proteins enriched by STL, LEL and WGA
Serum proteins enriched by solanum tuberosum lectin (STL), lycopersicon esculentum lectin (LEL) and wheat germ agglutinin (WGA) were analyzed by LC-DIA-MS/MS using Q-Exactive HF-X (ThermoFisher Scientific).
ORGANISM(S): Homo Sapiens (human) 
2024-02-24 | PXD048327 | JPOST Repository
ProteomicsProteome analysis of serum proteins enriched by MOA and PSL1a
We conducted serum protein enrichment using Marasmius oreades agglutinin (MOA) and Polyporus squamosus lectin (PSL1a), followed by analysis using HF-X.
ORGANISM(S): Homo Sapiens (human) 
2025-04-30 | PXD051875 | JPOST Repository
ProteomicsProteome analysis by simple and stable nanoESI spray system
We developed InSpIon system capable of stable nanoESI spray. Here, K562 cell digests were measured by DDA-MS and DIA-MS with the typical open nanoESI spray system (Open system) and the spray system inserted spray tip head into ion transfer tube (Insertion-ITT system), and the InSpIon system.
ORGANISM(S): Homo Sapiens (human) 
2023-04-05 | PXD039557 | JPOST Repository
ProteomicsIn-depth proteome analysis of Hela cell culture conditioned media containing fetal bovine serum.
We performed DIA-MS based proteomic analysis of Hela cells conditioned media containing fetal bovine serum by albumin (Alb)-depletion and non-depletion. In addition, we attempted to enrich secreted proteins by reducing the volume of culture medium (10mL, 5mL and 2mL). The supernatants of each cultur...
ORGANISM(S): Homo Sapiens (human) 
2022-02-22 | PXD024272 | JPOST Repository
ProteomicsDeep proteome analysis by DIA-MS
To expand proteome coverage, the MS range in DIA-MS was examined. In addition, we analyzed host proteins in the feces of control and repeated social defeat stress (R-SDS) model mice by DIA-MS with an optimal MS range.
ORGANISM(S): Homo Sapiens (human) Mus Musculus (mouse) 
2022-04-28 | PXD032698 | JPOST Repository
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