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ProteomicsWide-Window Acquisition Improves Proteome Coverage and Measurement Throughput for Label-Free Single-Cell Proteomics
The sensitivity of single-cell proteomics (SCP) has increased dramatically in recent years due to advances in experimental design, sample preparation, separations and mass spectrometry instrumentation. However, further increasing the sensitivity of SCP methods and instrumentation will enable the stu...
ORGANISM(S): Homo sapiens (Human) 
2023-07-11 | PXD037527 | Pride
Label-free Proteome Single-cell proteomics Wide-window acquisition 
ProteomicsHyperSCP: Combining Isotopic and Isobaric Labeling for Higher Throughput Single-Cell Proteomics
Recent developments in mass spectrometry-based single-cell proteomics (SCP) have resulted in dramatically improved sensitivity, yet the relatively low measurement throughput remains a limitation. Isobaric and isotopic labeling methods have been separately applied to SCP to increase throughput throug...
ORGANISM(S): Homo sapiens (Human) 
2023-07-11 | PXD040455 | Pride
Multiplex Single-cell proteomics High-throughput 
ProteomicsIn-depth mass spectrometry-based proteomics of formalin-fixed, paraffin embedded tissues with a spatial resolution of 50–200 µm
Formalin-fixed, paraffin-embedded (FFPE) tissues are banked in large repositories as a cost-effective means of preserving invaluable specimens for subsequent study, including for clinical proteomics in translational medicine. With the rapid growth of spatial proteomics, FFPE tissue samples can serve...
ORGANISM(S): Mus musculus (Mouse) 
2022-08-02 | PXD029729 | Pride
Fresh frozen Spatial Ddm Ffpe Lcm 
ProteomicsEasy and Accessible Workflow for Label-Free Single-Cell Proteomics
Single cell proteomics (SCP) can provide information that is unattainable through either bulk-scale protein measurements or single-cell profiling of other omes. Maximizing proteome coverage often requires custom instrumentation, consumables and reagents for sample processing and separations, which ...
ORGANISM(S): Homo sapiens (Human) 
2023-10-09 | PXD043355 | Pride
Exploris 480 Hp d100 single cell dispenser Single-cell proteomics 
ProteomicsImproved Single Cell Proteome Coverage Using Narrow-Bore Packed NanoLC Columns and Ultrasensitive Mass Spectrometry
we report on improved single-cell proteome coverage through the combination of the previously developed Nanodroplet Processing in One Pot for Trace Samples (nanoPOTS) platform with further miniaturization of liquid chromatography (LC) separations and implementation of an ultrasensitive latest-genera...
ORGANISM(S): Homo sapiens (Human) 
2020-02-26 | PXD016921 | Pride
Eclipse Narrow-bore lc Single cell 
ProteomicsMulticolumn Nanoflow Liquid Chromatography System with Accelerated Offline Gradient Generation for Robust and Sensitive Single-Cell Proteome Profiling
Peptide separations that combine high sensitivity, robustness, peak capacity and throughput are essential for extending bottom-up proteomics to smaller samples including single cells. To this end, we have developed a multicolumn nanoLC system with accelerated offline gradient generation. One binary ...
ORGANISM(S): Homo sapiens (Human) 
2025-05-06 | PXD049248 | Pride
High throughput Proteomics Lc-ms Storage loop Single cell proteomics Multiple columns 
ProteomicsThe $10 proteome: low-cost, deep and quantitative proteome profiling of limited sample amounts using the Orbitrap Astral and timsTOF Ultra 2 mass spectrometers
Mass spectrometry (MS)-based proteomics remains technically demanding and prohibitively expensive for many large-scale or routine applications, with per-sample costs of hundreds of dollars or more. To democratize access to proteomics and facilitate its integration into more high-throughput multiomic...
ORGANISM(S): Homo sapiens (Human) 
2026-02-04 | PXD066701 | Pride
Limited sample input $10 proteome 
ProteomicsFAIMS single cellUltrasensitive single-cell proteomics workflow identifies >1000 protein groups per mammalian cell
We report on the combination of nanodroplet sample preparation, ultra-low-flow nanoLC, high-field asymmetric ion mobility spectrometry (FAIMS), and the latest-generation Orbitrap Eclipse Tribrid mass spectrometer for greatly improved single-cell proteome coverage.
ORGANISM(S): Homo sapiens (Human) 
2020-11-24 | PXD019515 | Pride
Faims Single cell proteomics 
ProteomicsRapid, One-step Sample Processing for Label-Free Single-Cell Proteomics
Sample preparation for single-cell proteomics is generally performed in a one-pot workflow with multiple dispensing and incubation steps. These hours-long workflows can be labor intensive and lead to long sample-to-answer times. Here we report a sample processing method that achieves cell lysis, pro...
ORGANISM(S): Homo sapiens (Human) 
2023-10-09 | PXD041879 | Pride
Sample preparation Single-cell proteomics Lc-msms 
ProteomicsChromatography without a Binary Pump: Smoothed Step Gradients Enable Reproducible, Sensitive, and Low-Cost Nanoflow Separations
Mass spectrometry-based analysis of trace analytes, such as the single cell proteome, benefit from operation at low flow rates (i.e., <50 nL/min). However, the standard high-pressure binary pumps needed to achieve these flow rates are not commercially available, requiring splitting of the gradient f...
ORGANISM(S): Homo sapiens (Human) 
2025-05-07 | PXD052416 | Pride
Liquid chromatography Gradient formation Single-cell proteomics 
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