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UnknownIn-depth amino acid mutational analysis of the key interspecific incompatibility factor Stigmatic Privacy 1.
Not available
S-EPMC12290284 | biostudies-literature
ProteomicsPhosphoproteomics of nucleocapsid protein of SARS-CoV-2
We aimed to identify the functional phosphorylation of SARS-CoV-2 nucleoprotein. For this purpose, we used the Phos-tag technology to purify and enrich virus-derived phosphopeptides.
ORGANISM(S): Cellular Organisms 
2022-01-28 | PXD027939 | JPOST Repository
ProteomicsThe original mouse bone spectral library for the DIA-MS
We successfully constructed a customized spectral library containing information on 1,394 proteins for DIA-MS; the library can be used for comprehensive identification and quantification of proteins expressed in the mouse bones.
ORGANISM(S): Mus Musculus (mouse) 
PXD041731 | JPOST Repository
ProteomicsIdentification of gravity-responsive proteins in the femur of spaceflight mice using a quantitative proteomic approach
This project is “Medical proteome analysis of osteoporosis- and bone mass-related proteins using the Kibo Japanese Experiment Module of International Space Station (ISS)”. Utilizing proteomic technologies, this research aims to identify proteins involved in space flight-induced osteopenia as well as...
ORGANISM(S): Mus Musculus (mouse) 
PXD041738 | JPOST Repository
ProteomicsPlasminogen deficiency exacerbates skeletal muscle loss during mechanical unloading in developing mice
This project is “Medical proteome analysis of osteoporosis- and bone mass-related proteins using the Kibo Japanese Experiment Module of International Space Station (ISS)”. Applying proteomic technologies, this research aims to identify proteins involved in spaceflight-induced deterioration of muscul...
ORGANISM(S): Mus Musculus (mouse) 
2024-02-01 | PXD041806 | JPOST Repository
ProteomicsSelective Enrichment of Newly Synthesized Proteins using Phos-tag Click Tip Enables Nascent Proteome Analysis in Influenza A Virus Infection
Bioorthogonal non-canonical amino acid tagging (BONCAT)-based approaches enable selective labeling of newly synthesized proteins (NSPs). We developed a workflow termed the “Phos-tag Click Tip” by integrating a phosphorylated variant of bicyclononyne (pBCN) with Phos-tag affinity purification to sele...
ORGANISM(S): Homo Sapiens (human) 
PXD059239 | JPOST Repository
ProteomicsThe customized mouse serum spectral library for the DIA-MS
For a comprehensive analysis of the mouse serum proteome, a customized mouse serum spectral library was constructed and utilized for quantitative analysis using DIA-MS. Our spectral library contained information on 1,106 mouse serum proteins. Creation of the spectral library was performed using Spec...
ORGANISM(S): Mus Musculus (mouse) 
2024-03-16 | PXD041889 | JPOST Repository
ProteomicsIdentification of gravity-responsive serum proteins in spaceflight mice using a quantitative proteomic approach with data-independent acquisition mass spectrometry
Physical inactivity associated with gravity unloading, such as microgravity during spaceflight and hindlimb unloading (HU), can cause various physiological changes. In this study, we attempted to identify serum proteins whose levels fluctuated in response to gravity unloading. First, we quantitative...
ORGANISM(S): Mus Musculus (mouse) 
2024-03-16 | PXD041894 | JPOST Repository
ProteomicsCharacterization of phosphopeptide enrichment strategy for mass spectrometry-based proteomic analysis
We attempted to evaluate four strategies for phosphopeptide enrichment, using the titanium dioxide (TiO2) and Phos-tag ligand particles, from in-solution or in-gel digests prior to mass spectrometry-based proteomic analysis.
ORGANISM(S): Homo Sapiens (human) Cellular Organisms 
2021-12-24 | PXD026295 | JPOST Repository
ProteomicsIdentification of gravity-responsive proteins in mouse soleus muscle using proteomic analysis
This project is “Medical proteome analysis of osteoporosis- and bone mass-related proteins using the Kibo Japanese Experiment Module of International Space Station (ISS)”. Applying proteomic technologies, this research aims to identify proteins involved in space flight-induced osteopenia as well as ...
ORGANISM(S): Mus Musculus (mouse) 
PXD041190 | JPOST Repository
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