KKT23 is a kinetoplastid kinetochore protein that has structural similarity to the GCN5 acetyltransferase domain. Using a kinetoplastid parasite Trypanosoma brucei, here we performed immunoprecipitation and mass spectrometry of GFP-tagged KKT23 N-terminal fragment (2–150) to identify co-purifying pr...
The kinetochore is the macromolecular protein machine that drives chromosome segregation by interacting with spindle microtubules. Unlike most other eukaryotes that have canonical kinetochore proteins, a group of evolutionarily divergent eukaryotes called kinetoplastids (such as Trypanosoma brucei t...
In spite of their universal function, the composition of kinetochores differs greatly across eukaryotes. Trypanosomes and other organisms Kinetoplastida diverged very early during eukaryotic radiation from the line that includes animals and fungi, and build kinetochores with very little detectable h...
