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We found that a number of Tfh cells downmodulated BCL6 protein after their development, and we sought to compare the gene expression between BCL6-hi Tfh cells and BCL6-low Tfh cells. CD4+ T cells were sorted from immunized and non-immunized mice for RNA extraction and hybridization on Affymetrix mic...
ORGANISM(S): Mus musculus 
We applied AP-MS to quantify Grb2 interaction dynamics in primary T cells. Data generated here in shotgun/DDA mode were used to build a high quality Grb2 binders-specific SWATH assay library for high-throughput targeted analysis of DIA data.
ORGANISM(S): Mus musculus (Mouse) 
2017-03-09 | PXD003973 | Pride
We applied AP-SWATH to quantify Grb2 interaction dynamics in primary T cells with a high level of reproducibility and accuracy. We concluded that AP-SWATH is a robust approach to quantify signaling dynamics in primary cells and could be further expanded to additional hub proteins using a range of ce...
ORGANISM(S): Mus musculus (Mouse) 
2017-03-09 | PXD003972 | Pride
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