We performed mass spectrometry of a recombinant KKT14 N-terminal fragment that was phosphorylated by AUK1 (Aurora B homolog in Trypanosoma brucei) in vitro to identify phosphorylation sites.
We performed immunoprecipitation and mass spectrometry of kinetoplastid kinetochore proteins (full-length proteins for KKT4, KKT14, and KKT22 as well as KKT14 fragments) to identify co-purifying proteins.
