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ProteomicsPuromycin acetyltransferase-mediated cell-specific labeling of de novo synthesized proteins, part I
Puromycin and its derivative O-propargyl puromycin (OPP) enable detection of nascent proteins. Use of these metabolic labels in complex mixtures of cells leads to indiscriminate tagging of nascent proteomes independent of cell type. Here we have used puromycin N-acetyltransferase (PAT), for cell-spe...
ORGANISM(S): Mus musculus (Mouse) Homo sapiens (Human) 
2023-10-24 | PXD043494 | Pride
Neuro2a Hek Opp-id Shotgun proteomics Streptavidin pulldown 
ProteomicsPuromycin acetyltransferase-mediated cell-specific labeling of de novo synthesized proteins, Part II
Puromycin and its derivative O-propargyl puromycin (OPP) enable detection of nascent proteins. Use of these metabolic labels in complex mixtures of cells leads to indiscriminate tagging of nascent proteomes independent of cell type. Here we have used puromycin N-acetyltransferase (PAT), for cell-spe...
ORGANISM(S): Rattus rattus (Black rat) 
2023-10-24 | PXD043498 | Pride
Opp-id Shotgun proteomics Streptavidin pulldown Rat cortical neuron-glia co-culture 
ProteomicsSILAC analysis of the effects of AS1411 on 3T3 cell proteome.
Here we conducted a SILAC analysis to study the effects of AS1411 on 3T3 cell proteome, and found that AS1411 consistently downregulated the expression of cholesterol biosynthesis enzymes
ORGANISM(S): Mus musculus (Mouse) 
2025-10-17 | PXD055162 | Pride
Cholesterol biosynthesis pathway Nucleolin As1411 Aptamer 
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