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ProteomicsHEK293 Ku70 WT BioID - Mapping the Ku interactome using proximity-dependent biotin identification in human cells
We conducted proximity-dependent biotin identification (BioID) using wild-type Ku70 in HEK293 cells. Ku70 is part of a protein complex known as Ku, which is involved in a variety of functions throughout the cell. By identifying candidate protein interactors, we can infer new cellular processes that ...
ORGANISM(S): Homo sapiens (Human) 
2019-01-03 | PXD010931 | Pride
Ku70 Bioid Hek293 
TranscriptomicsKu70 Is a Novel Cytosolic DNA Sensor that Induces Type-III Rather than Type-I IFN
Cytosolic foreign DNA is detected by pattern recognition receptors and mainly induces Type-I IFN production. We found that transfection of different types of DNA into various untreated cells induces Type-III IFN (IFN-lambda1) rather than Type-I IFN, indicating the presence of uncharacterized DNA sen...
ORGANISM(S): Homo sapiens 
2011-01-29 | E-GEOD-26944 | biostudies-arrayexpress
ProteomicsUsing BioID2 to map domain specific Ku70 protein interactors
To determine if the proximity-dependent biotin identification (BioID2) technique could identify and map domain and region specific protein interactors, we conducted BioID2 with various segments of the Ku70 protein in the HEK293 cell line and compared the different datasets. To our knowledge, this is...
ORGANISM(S): Homo sapiens (Human) 
2021-04-22 | PXD020165 | Pride
Ku70 Bioid2 Hek293 Lc-esi-ms/ms 
ProteomicsGeneration and Characterization of a Conditional Ku70 Knockout Cell Line
In this study we characterize and investigate the effect of the loss of Ku70 protein in a conditional Ku70 knockout TREx-293 cell system. Loss of Ku70 protein levels was directly correlated with a loss of cell viability, supporting the observation that Ku performs an essential role in human cells. I...
ORGANISM(S): Homo sapiens (Human) 
2023-07-27 | PXD036297 | Pride
Ku70 hek293 
ProteomicsHuman Ku70 WT AP-MS - Mapping the Ku interactome using proximity-dependent biotin identification in human cells
Affinity purification coupled to mass spectrometry is often used as a complementary technique to BioID experiments, and the data presented here complements another project. To infer processes involving Ku70, we used AP-MS to identify candidate proteins that may interact with Ku70 and compared these ...
ORGANISM(S): Homo sapiens (Human) 
2019-01-03 | PXD010930 | Pride
Ku70 Ap-ms Hek293 
UnknownIdentification of Ku70 Domain-Specific Interactors Using BioID2.
Not available
S-EPMC8001828 | biostudies-literature
ProteomicsPAXX is an accessory c-NHEJ factor that associates with Ku70 and has overlapping functions with XLF
In mammalian cells, classical non-homologous end joining (c-NHEJ) is critical for DNA double-strand break repair induced by ionizing radiation and during V(D)J recombination in developing B and T lymphocytes. Recently, PAXX was identified as a new c-NHEJ core component. We report here, that PAXX-def...
ORGANISM(S): Homo sapiens (Human) 
2016-10-06 | PXD004659 | Pride
Non-homologous end joining Micro-homology mediated end joining V(d)j recombination Genomic instability Dna double-strand break 
GenomicsHomo sapiens
Ku70 Is a Novel Cytosolic DNA Sensor that Induces Type-III Rather than Type-I IFN
PRJNA136943 | ENA
UnknownPurification and identification of positive regulators binding to a novel element in the c-Jun promoter.
Not available
S-EPMC2905795 | biostudies-literature
UnknownCDCA7 and HELLS mutations undermine nonhomologous end joining in centromeric instability syndrome.
Not available
S-EPMC6307953 | biostudies-literature
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