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ProteomicsPharmacological perturbation of the phase-separating protein SMNDC1
SMNDC1 is an essential splicing factor that, like its better-studied paralog SMN, binds to di-methylated arginines using its Tudor domain. The specific contributions of the SMNDC1 Tudor domain to protein-protein interactions, subcellular localization, and molecular function have not been studied in ...
ORGANISM(S): Mus musculus (Mouse) 
2023-09-04 | PXD037092 | Pride
Pancreatic alpha cells Tudor domain Small molecule inhibitor Nmr structure Nuclear speckles Splicing Chemical screening 
ProteomicsSMNDC1 links chromatin remodeling and splicing to regulate pancreatic hormone expression
Insulin expression is restricted to the pancreatic beta cells, which are physically or functionally depleted in diabetes. Identifying targetable pathways repressing insulin in non-beta cells, particularly in the developmentally related glucagon-secreting alpha cells, is an important aim of regenerat...
ORGANISM(S): Mus musculus (Mouse) 
2022-07-01 | PXD030363 | Pride
Pancreatic islets Chromatin remodelers Alpha cells Beta cells Smndc1 Rnai screen Splicing Insulin transcription 
ProteomicsSMNDC1 links chromatin remodeling and splicing to regulate pancreatic hormone expression
Insulin expression is restricted to the pancreatic beta cells, which are physically or functionally depleted in diabetes. Identifying targetable pathways repressing insulin in non-beta cells, particularly in the developmentally related glucagon-secreting alpha cells, is an important aim of regenerat...
ORGANISM(S): Mus musculus (Mouse) 
2022-07-01 | PXD030362 | Pride
Pancreatic islets Chromatin remodelers Alpha cells Beta cells Smndc1 Rnai screen Splicing Insulin transcription 
ProteomicsSMNDC1 links chromatin remodeling and splicing to regulate pancreatic hormone expression
Insulin expression is restricted to the pancreatic beta cells, which are physically or functionally depleted in diabetes. Identifying targetable pathways repressing insulin in non-beta cells, particularly in the developmentally related glucagon-secreting alpha cells, is an important aim of regenerat...
ORGANISM(S): Mus musculus (Mouse) 
2022-07-01 | PXD030361 | Pride
Pancreatic islets Chromatin remodelers Alpha cells Beta cells Smndc1 Rnai screen Splicing Insulin transcription 
ProteomicsSystematic characterization of BAF mutations explains intra-complex synthetic lethalities
Aberrations in genes coding for subunits of the BAF chromatin remodeling complex are highly abundant in human cancers. Currently, it is not understood how these loss-of-function mutations contribute to cancer development and how they can be targeted therapeutically. The cancer type specific occurren...
ORGANISM(S): Homo sapiens (Human) 
2019-08-01 | PXD013102 | Pride
Atac-seq Interaction proteomics Chromatin remodeling Baf complex Chromatin accessibility Chromatin binding Transcriptomics Synthetic lethality Mammalian swi/snf complex 
ProteomicsAcute BAF perturbation causes immediate changes in chromatin accessibility
Loss-of-function mutations in genes coding for subunits of the large, multifarious BRG1/BRM associated factor (BAF) chromatin remodeling complexes are frequently causative for cancer or developmental diseases1-5. Cells lacking the most frequently mutated subunits like the ATPase SMARCA4 typically ex...
ORGANISM(S): Homo sapiens (Human) 
2020-12-23 | PXD018381 | Pride
Atac-seq Enhancer Dtag knock-in Chromatin remodeling Baf complex Chromatin accessibility Acute chemical degradation Synthetic lethality Mammalian swi/snf complex Kinetics 
ProteomicsChromatin isolation for mass spectrometry
The histone acetyl-reader BRD4 is an important regulator of chromatin structure and transcription, yet factors modulating its activity have remained elusive. Here we describe two complementary screens for genetic and physical interactors of BRD4, which converge on the folate pathway enzyme MTHFD1. W...
ORGANISM(S): Homo sapiens (Human) 
2019-04-04 | PXD013090 | Pride
Transcription Brd4 Mthfd1 Folate metabolism Gene regulation 
TranscriptomicsSingle-cell transcriptomics reveals unique features of human pancreatic islet cell subtypes
We report the single-cell RNA-seq based identification of 6 known human islet cell types (alpha cells, beta cells, delta cells, pp cells, acinar cells and duct cells) based on the expression of known marker genes. We further assess cell type specific gene expression and suggest novel marker genes fo...
ORGANISM(S): Homo sapiens 
2016-01-05 | E-GEOD-73727 | biostudies-arrayexpress
ProteomicsBFP pulldown of BFP-NUDT5 HAP1 MTHFD1-K56R cells
We performed BFP pulldowns with BFP-tagged NUDT5 in HAP1 harboring the MTHFD1 K56R mutation to find interactors of NUDT5. We performed the study under various medium conditions, including full IMDM medium (FULL), IMDM with dialysed FBS (DIA) and DIA supplemented with 50µM adenosine to investigate po...
ORGANISM(S): Homo sapiens (Human) 
2025-11-07 | PXD067739 | Pride
TranscriptomicsNOTCH1 activation in breast cancer confers sensitivity to inhibition of SUMOylation
Breast cancer is genetically heterogeneous, and recent studies have underlined a prominent contribution of epigenetics to the development of this disease. To uncover new synthetic lethalities with known breast cancer oncogenes, we screened an epigenome-focused short hairpin RNA library on a panel...
ORGANISM(S): Homo sapiens 
2014-11-21 | E-GEOD-61727 | biostudies-arrayexpress
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