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MetabolomicsAutomated Label-free Quantification of Metabolites from Liquid Chromatography–Mass Spectrometry Data (Simulated)

Liquid chromatography coupled to mass spectrometry (LC-MS) has become a standard technology in metabolomics. In particular, label-free quantification based on LC-MS is easily amenable to large-scale studies and thus well suited to clinical metabolomics. Large-scale studies, however, require autom...

2015-12-16 | MTBLS235 | MetaboLights
MetabolomicsAutomated Label-free Quantification of Metabolites from Liquid Chromatography–Mass Spectrometry Data (Plasma)

Liquid chromatography coupled to mass spectrometry (LC-MS) has become a standard technology in metabolomics. In particular, label-free quantification based on LC-MS is easily amenable to large-scale studies and thus well suited to clinical metabolomics. Large-scale studies, however, require autom...

2015-12-16 | MTBLS234 | MetaboLights
ProteomicsCombining label-free and label-based accurate quantifications with SWATH-MS: comparison with SRM and PRM for the evaluation of bovine muscle type effects.
Mass spectrometry has proven to be a valuable tool for the accurate quantification of proteins. In this study, we have evaluated the performances of three targeted approaches, namely Selected Reaction Monitoring (SRM), Parallel Reaction Monitoring (PRM) and Sequential Windowed Acquisition of Theoret...
ORGANISM(S): Bos Taurus 
2021-06-08 | PXD020680 | panorama
ProteomicsSurfactant-assisted one-pot sample preparation for label-free single-cell proteomics
Large numbers of cells are generally required for quantitative global proteome profiling due to the significant surface adsorption losses associated with sample processing. Such bulk measurement obscures important cell-to-cell variability (cell heterogeneity) and makes proteomic profiling impossible...
ORGANISM(S): Homo Sapiens 
2021-03-11 | PXD022827 | panorama
ProteomicsIdentification of stably expressed reference genes for expression studies in Arabidopsis thaliana using mass spectrometry-based label-free quantification
Arabidopsis thaliana has been used regularly as a model plant in gene expression studies on transcriptional reprogramming upon pathogen infection, such as that by Pseudomonas syringae pv. tomato DC3000 (Pst DC3000), or when subjected to stress hormone treatments including jasmonic acid (JA), salicyl...
ORGANISM(S): Arabidopsis thaliana (Mouse-ear cress) 
2022-09-05 | PXD035677 | Pride
Pathogen infection  jasmonic acid (ja)  salicylic acid (sa)  abscisic acid (aba)  expression study  label-free quantification (lfq)  rt-qpcr  reference gene 
UnknownImputation of label-free quantitative mass spectrometry-based proteomics data using self-supervised deep learning.
Not available
S-EPMC11208500 | biostudies-literature
ProteomicsMass Dynamics 1.0: A streamlined, web-based environment for analyzing, sharing and integrating Label-Free Data
Label Free Quantification (LFQ) of shotgun proteomics data is a popular and robust method for the characterization of relative protein abundance between samples. Many analytical pipelines exist for the automation of this analysis and some tools exist for the subsequent representation and inspection ...
ORGANISM(S): Homo sapiens (Human) Escherichia coli Saccharomyces cerevisiae (Baker's yeast) 
2022-04-13 | PXD028038 | Pride
Maxquant Benchmarking Label-free quantification Web-based software tool Automated data analysis 
ProteomicsLabel-free Quantification of Proteins in Single Embryonic Cells with Neural Fate in the Cleavage-Stage Frog (Xenopus laevis) Embryo using CE-ESI-HRMS
In this work, we integrate capillary electrophoresis electrospray ionization mass spectrometry, bottom-up proteomics, and single-cell microanalysis to enable the label-free quantification (LFQ) of proteins in single embryonic cells (D11 blastomeres) in the 16-cell frog (Xenopus laevis) embryo. Based...
ORGANISM(S): Xenopus laevis (African clawed frog) 
2016-06-23 | PXD004174 | Pride
Blastomere Embryo Proteomics Xenopus Mass spectrometry Fusion Label free quantification Single cell Lfq Capillary electrophoresis Electrospray ionization Impact hd 
ProteomicsLabel free quantitative mass spectrometry identifies platelet degranulation as an early event during platelet storage
Shelf life of platelet concentrates (PCs) is limited to up to 7 days due to loss of platelet functionality during storage, commonly referred to as the platelet storage lesion (PSL). To get more insight into the development of the PSL, we used label free quantitative mass spectrometry to identify cha...
ORGANISM(S): Homo sapiens (Human) 
2017-09-11 | PXD005610 | Pride
Proteomics Label free quantification Alpha-granules Platelet storage Platelets 
ProteomicsIntegrated analysis of label-free quantitative proteomics and bioinformatics reveal insights into signaling pathways in male breast cancer
The project contains raw and result files from a proteomic profiling of a male breast cancer (MBC) case. Label-free quantification-mass spectrometry (LFQ-MS) and bioinformatics analysis were employed to investigate the differentially expressed proteins (DEPs) among distinct tissue samples: the prima...
ORGANISM(S): Homo sapiens (Human) 
2021-09-08 | PXD012453 | Pride
Proteomics Signaling pathways Label-free mass spectrometry Bioinformatics analysis Male breast cancer 
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