In order to identify sites of the SEC16A specifically phosphorylated by the Dual Specificity Kinase DYRK3, mCherry-SEC16A was transiently over expressed in HeLa cells together with GFP-DYRK3 or EGFP-C1 only
Stable cell line was used for this study, so there are minimum variations between samples. For the lable free based quantitative analysis of the whole proteome, three biological replicates for each condition were investigated. For the phosphoproteomic analysis, three biological replicates and two te...
The 4D-lable free proteomic and acetylomic study were employed to gain the targets of GS. The co-immunoprecipitation, co-localization, and specific acetylation site antibody were used to verify the acetylation. Based on the mass spectrometry analysis, the active monomers in GS were screened by pheno...
APEX1 overexpression and knockdown cell lines were established based on SKOV3 cell line. Lable-free quantitative phosphoproteomics was done to evaluate the impact of APEX1 on cellular phosphoproteomics
In order to elucidate the potential mechanism of resistance of active substances to PAO1, we performed a 4D Lable Free proteomic analysis to compare the differentially expressed proteins of PAO1 under the stress of active substances.
Pepper(Capsicum annuum L.) fruit development is a complex and genetically programmed process, a comparative study of transcriptome and proteome changes during two varieties of pepper development（IMG, MG, Br and MR） has been carried out by using RNA-Seq and Lable-free quantitation technology.
