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ProteomicsMicro- and macro- heterogeneity of N-glycoproteome remodeling in endoplasmic reticulum stress
We developed an optimized magnetic polyethyleneimine boronic acid-containing scaffold (mPBA) enrichment workflow to achieve sensitive and broad enrichment of intact glycoproteins for mass spectrometry analysis, quantifying 13759 unique protein-, site-, and glycoform combinations, termed glycopeptido...
ORGANISM(S): Homo sapiens (Human) 
2026-10-19 | PXD079324 | Pride
Boronic acid Ipsc Ac16 Intact glycopeptides N-glycoproteome Er stress 
ProteomicsEffects of age and sex on protein abundance in the mouse heart
We compared protein abundance in the left and right ventricles of male and female C57BL6/J mice at 4 months and 20 months old. Frozen tissues were weighed, thawed, and cut into small pieces (2-3 mm3) on weigh boats on ice. Tissue pieces were transferred to 2.0 mL eppendorf tubes. Cold RIPA (Thermo #...
ORGANISM(S): Mus Musculus (mouse) 
2022-11-08 | PXD033719 | JPOST Repository
ProteomicsHuman Heart Whole Tissue Lysate LC-MS/MS
Commercial human heart whole tissue lysates were analyzed with LC-MS/MS with an inclusion list of alternative sequences (Lau et al. bioRxiv 2019).
ORGANISM(S): Homo sapiens (Human) 
2019-12-16 | PXD015544 | Pride
Human Heart Lc-ms/ms 
ProteomicsHuman induced pluripotent stem cell differentiation to cardiomyocytes
Shotgun proteomics analysis of directed differentiation of human induced pluripotent stem cells to cardiomyocytes
ORGANISM(S): Homo sapiens (Human) 
2019-12-16 | PXD013426 | Pride
Cardiomyocytes Ipsc Ipsc-cm 
ProteomicsProteomic profiles of fetal and postnatal mouse hearts
Fetal heart and postnatal hearts (n=5 each) were acquired commercially at E17.5 and P1 from Zyagen (San Diego, CA), weighed, and homogenized for proteomics profiling. Tissue homogenization was performed with a handheld homogenizer (OMNI International). Samples were placed on ice for 1 minute to allo...
ORGANISM(S): Mus Musculus (mouse) 
2025-07-23 | PXD052640 | JPOST Repository
ProteomicsProteomic profiles of adult, aged, and hypertrophic mouse hearts
All animal protocols were approved by the Institutional Animal Care and Use Committee (IACUC) guidelines at the University of Colorado Denver/Anschutz Medical Campus. For normal and hypertrophic mouse hearts, wild-type C57BL/6J mice were purchased from Jackson Laboratories (Bar Harbor, ME, USA) and ...
ORGANISM(S): Mus Musculus (mouse) 
2025-07-23 | PXD052641 | JPOST Repository
ProteomicsPrimary human cardiac ventricular fibroblasts
Transformed AC16 cells were acquired from Millipore and used at P10-15 and expanded in DMEM/F-12 with 10% FBS (Gibco). Primary human ventricular cardiac fibroblasts were acquired from Promocell and expanded in FGM-3 (Promocell) and used at P3-5. For the experiment, cells were separately placed on tr...
ORGANISM(S): Homo Sapiens (human) 
2023-05-05 | PXD041982 | JPOST Repository
ProteomicsCalibration and heavy water labeling in normal and stressed human AC16 cells
For calibration curve samples, human AC16 cells (Millipore) were cultured in DMEM/F12 supplemented with 10% FBS and either 6% D2O (heavy labeled population) or 6% H2O (control population) at 37°C, 5% CO2. The cells were maintained in this medium for 3 passages, each passage with a split ratio of 1:8...
ORGANISM(S): Homo Sapiens (human) 
2025-07-23 | PXD048321 | JPOST Repository
ProteomicsLongevity of cardiac and skeletal muscle proteins
Mice were injected with two 500μL injections of 0.15M NaCl dissolved in 99.9% D2O, separated by a four-hour interval. Then, the mice were given ad libitum access to 8% D2O drinking water for the remainder of the experiment. Mice were euthanized and dissected on days 0, 1, 2, 3, 4, 6, 8, 10, 15, 20, ...
ORGANISM(S): Mus Musculus (mouse) 
2026-04-26 | PXD063579 | JPOST Repository
ProteomicsHuman induced pluripotent stem cells under dynamic heavy water labeling
Fractional synthesis calibration cells for hiPSC AICS-0052-003 hiPSC (mono-allelic C-terminus mEGFP-tagged MYL7 WTC-11; Allen Institute Cell Collection) line was acquired from Coriell Institute and seeded onto Geltrex (Gibco) coated 6 well plates and maintained in mTesR Plus basal media with Supplem...
ORGANISM(S): Homo Sapiens (human) 
2025-07-23 | PXD060287 | JPOST Repository
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