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ProteomicsPlant SILAC: Stable-Isotope Labelling with Amino Acids of Arabidopsis Seedlings for Quantitative Proteomics.
Stable Isotope Labelling by Amino acids in Cell culture (SILAC) is a powerful technique for comparative quantitative proteomics, which has recently been applied to a number of different eukaryotic organisms. Inefficient incorporation of labelled amino acids in cell cultures of Arabidopsis thaliana h...
ORGANISM(S): Arabidopsis Thaliana (ncbitaxon:3702) 
2016-08-15 | MSV000080043 | MassIVE
ProteomicsProteomic Analysis of the Response to Cell Cycle Arrests in Human Myeloid Leukemia Cells
Previously (Ly et al. 2014), we analyzed protein abundance changes across a ‘minimally perturbed’ cell cycle in human cells (NB4), using centrifugal elutriation to differentially enrich distinct cell cycle phases. Here, we compare data from elutriated cells with NB4 cells arrested at comparable ...
ORGANISM(S): Homo Sapiens (ncbitaxon:9606) 
2017-03-31 | MSV000080841 | MassIVE
ProteomicsEnhanced snoMEN vectors facilitate establishment of GFP–HIF-1? protein replacement human cell lines
The snoMEN (snoRNA Modulator of gene ExpressioN) vector technology was developed from a human box C/D snoRNA, HBII-180C, which contains an internal sequence that can be manipulated to make it complementary to RNA targets, allowing knock-down of targeted genes. Here we have screened additional human ...
ORGANISM(S): Homo Sapiens (ncbitaxon:9606) 
2017-03-31 | MSV000080828 | MassIVE
ProteomicsGlobal Subcellular Characterization of Protein Degradation Using Quantitative Proteomics
Protein degradation provides an important regulatory mechanism used to control cell cycle progression and many other cellular pathways. To comprehensively analyze the spatial control of protein degradation in U2OS osteosarcoma cells, we have combined drug treatment and SILAC-based quantitative mass ...
ORGANISM(S): Homo Sapiens (ncbitaxon:9606) 
2017-03-31 | MSV000080816 | MassIVE
ProteomicsAge-related changes in protein abundance in nematodes
Using quantitative mass spectrometry together with protein and peptide-level fractionation, we can now report a major increase in coverage of the C. elegans proteome, identifying over 9,300 proteins in three biological replicates. The resulting data have been assembled into a searchable, online reso...
ORGANISM(S): Caenorhabditis Elegans (ncbitaxon:6239) 
2015-08-21 | MSV000079263 | MassIVE
ProteomicsGlobal Membrane Protein Interactome Analysis using In vivo Crosslinking and MS-based Protein Correlation Profiling
We present a methodology using in vivo crosslinking combined with HPLC-MS for the global analysis of endogenous protein complexes by protein correlation profiling. Formaldehyde crosslinked protein complexes were extracted with high yield using denaturing buffers that maintained complex solubility du...
ORGANISM(S): Homo Sapiens (ncbitaxon:9606) 
2016-08-02 | MSV000080014 | MassIVE
ProteomicsHipSci project proteomics batch 1, comprising 6 IPS cell lines from 3 donors
The Human Induced Pluripotent Stem Cells Initiative (HipSci) is generating a large, high-quality reference panel of human IPSC lines. This is a submission of mass-spectrometry analyses from 6 induced pluripotent stem cell lines generated by the HipSci project.
ORGANISM(S): Homo Sapiens (ncbitaxon:9606) 
2017-05-15 | MSV000081088 | MassIVE
ProteomicsA quantitative spatial proteomics analysis of proteome turnover in human cells
Measuring the properties of endogenous cell proteins, such as expression level, subcellular localization and turnover rates, on a whole proteome level remains a major challenge in the post-genome era. Quantitative methods for measuring mRNA expression do not reliably predict corresponding protein le...
ORGANISM(S): Homo sapiens (Human) 
2015-12-01 | PXD003258 | Pride
Hela Subcellular Silac 
ProteomicsGlobal proteomics analysis reveals a role for histone variant H3.3 in the response to starvation
Periodic starvation of animals induces large shifts in metabolism, but may also influence many other cellular systems and can lead to adaption to prolonged starvation conditions. To date, there is limited understanding of how starvation affects gene expression, particularly at the protein level. Her...
ORGANISM(S): Caenorhabditis elegans 
2015-06-17 | PXD001723 | Pride
C. elegans Starvation Histone 
ProteomicsA protein phosphatase network controls the temporal and spatial dynamics of differentiation commitment in human epidermis
Epidermal homeostasis depends on a balance between stem cell renewal and terminal differentiation. The transition between the two cell states,termed commitment, is poorly understood. Here we characterise commitment by integrating transcriptomic and proteomic data from disaggregated primary human ker...
ORGANISM(S): Homo sapiens (Human) 
2017-10-18 | PXD003281 | Pride
Human Timecourse Keratinocytes Pkc U2os 
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