Sort   by:  
 Page size 
ProteomicsCombining laser capture micro-dissection and proteomics reveals an active translation machinery controlling invadosome formation
We developed a method that combines laser micro-dissection and mass spectrometry, enabling the analysis of subcellular structures in their native state based on low amounts of input material. Using this combinatorial method, we showed that invadosomes contain specific components of the translational...
ORGANISM(S): Mus musculus (Mouse) 
2018-10-19 | PXD009390 | Pride
Invadosome Mass spectrometry Laser micro-dissection Translation 
TranscriptomicsLaser Capture Microdissection isolation of preovulatory granulosa cells from WT and bERKO ovaries
Determining the spatial and temporal expression of genes involved in the ovulatory pathway is critical for the understanding of the role of each estrogen receptor in the modulation of folliculogenesis and ovulation. Estrogen receptor (ER) b is highly expressed in ovarian granulosa cells and mice lac...
ORGANISM(S): Mus musculus 
2013-07-03 | E-GEOD-44651 | biostudies-arrayexpress
TranscriptomicsMouse sinoatrial node transcriptome
RNA Sequencing of Mouse Sinoatrial Node Reveals an Upstream Regulatory Role for Islet-1 in Cardiac Pacemaker Cells Rationale: Treatment of sinus node disease with regenerative or cell-based therapies will require a detailed understanding of gene regulatory networks in cardiac pacemaker cells (PCs)....
ORGANISM(S): Mus musculus 
2015-02-06 | E-GEOD-65658 | biostudies-arrayexpress
TranscriptomicsEmbryo methylation profiling in marmosets
Here we perform Bisulfite sequencing (BS-seq) on marmoset post-implantation embryos following laser capture micro dissection to capture methylation profiles of post-implantation tissues. We also perform single-cell BS-seq on pre-implantation marmoset embryos from four-cell to the compacted morula st...
ORGANISM(S): Callithrix jacchus 
2024-09-22 | E-MTAB-13893 | biostudies-arrayexpress
TranscriptomicsGene expression profiles of components isolated from developing kidney at E11.5, E12.5 & E15.5 using LCM. (GUDMAP Series ID: 10)
The long term objective is to create an encyclopedia of the expression levels of all genes in multiple components of the developing kidney. The central thesis is straightforward. The combination of laser capture microdissection (LCM) plus microarray analysis offers a powerful, efficient and effecti...
ORGANISM(S): Mus musculus 
2006-11-18 | E-GEOD-6290 | biostudies-arrayexpress
TranscriptomicsTranscription profiling of Drosophila EcR-dependent gene expression in MB neurons at the onset of axon pruning
This study identifies genes that show EcR-dependent gene expression in MB neurons at the onset of axon pruning. Experiment Overall Design: A dominant negative form of the ecdysone receptor (EcRDN) was expressed in MB neurons by OK107-GAL4. Animals were staged at 0 and 5 hours relative to puparium f...
ORGANISM(S): Drosophila melanogaster 
2008-06-18 | E-GEOD-10013 | biostudies-arrayexpress
TranscriptomicsAgilent Microarray of laser capture microdissected mesenteric adipose tissue from C57BL/6J mice with repeated endoscopic colon biopsies or from uninjured mice
We performed repeated endoscopic colon biopsies of C57BL/6J mice weekly and found that the mesenteric adipose tissue (MAT) was enlarged and it wrapped around the colon at wound sites after 4 cycles of biopsy. On histological view, fibrous bands extended from the intestine into the adjacent MAT, mimi...
ORGANISM(S): Mus musculus 
2021-07-16 | E-MTAB-8566 | biostudies-arrayexpress
TranscriptomicsTranscription profiling of Drosophila mushroom body neurons at the onset and early steps of axon pruning
Drosophila mushroom body (MB) γ neurons undergo axon pruning during metamorphosis through a process of localized degeneration of specific axon branches. Developmental axon degeneration is initiated at the onset of metamorphosis by the pre-pupal rise in the steroid hormone ecdysone. This study ide...
ORGANISM(S): Drosophila melanogaster 
2008-06-18 | E-GEOD-10012 | biostudies-arrayexpress
ProteomicsProteomics on formalin-fixed and stained laser-capure microdissected human lung sections
A broad range of tissues have been examined by microscopy following a process of formalin fixation, paraffin embedment, sectioning and staining. Haematoxylin and eosin (H&E) – which respectively stain nuclei blue and other cellular and stromal material pink – are routinely used for clinical diagnosi...
ORGANISM(S): Homo sapiens (Human) 
2021-09-08 | PXD014762 | Pride
Lung Human Laser-capture Dissection Spatial proteomics Fixed 
TranscriptomicsTranscription profiling by array of Citrus clementina laser microdissected epidermis and subepidermis from expanding green fruit rind
Transcriptomic profiling of laser microdissected epidermis and subepidermis cells from expanding green fruit rind of Citrus clemenules using the cDNA microarray CFGP-Citrus_20k.
ORGANISM(S): Citrus clementina 
2010-05-23 | E-TABM-954 | biostudies-arrayexpress
Sort   by:  
 Page size