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ProteomicsBenchmarking LFQ, SILAC and MS2/MS3-based TMT quantification strategies for large-scale phosphoproteomics
Comprehensive mass spectrometry (MS)-based proteomics is now feasible, but reproducible and multiplexed quantification remains challenging especially for analysis of post-translational modifications (PTMs), such as phosphorylation. Here we compared the most popular quantification techniques for phos...
ORGANISM(S): Homo sapiens (Human) Saccharomyces cerevisiae (Baker's yeast) 
2018-03-13 | PXD007145 | Pride
Phosphoproteomics Ms3 Ms2 Tmt Silac Lfq Technical Benchmark Quantification 
ProteomicsComparative assessment of quantification methods for tumor tissue phosphoproteomics
The choice of quantification method is critical for study design and comparison of cancer phosphoproteomes. We comparatively assessed label-free quantification (LFQ), spike-in-SILAC (stable isotope labeling of amino acids in cell culture) and tandem mass tag (TMT) labeling techniques for quantitativ...
ORGANISM(S): Homo sapiens (Human) 
2022-08-09 | PXD030450 | Pride
Phosphoproteomics Tissue Ovarian cancer Quantification 
UnknownLabel-Free Quantitative Phosphoproteomics of the Fission Yeast Schizosaccharomyces pombe Using Strong Anion Exchange- and Porous Graphitic Carbon-Based Fractionation Strategies.
Not available
S-EPMC7916215 | biostudies-literature
ProteomicsLabel-Free Phosphoproteomics Profiling of Fission Yeast Schizosaccharomyces pombe
The phosphorylation of proteins modulates various functions of proteins and plays an important role in regulation of cell signaling. In the recent years, the label-free quantitative (LFQ) phos-phoproteomics has become the powerful tool to analyze the phosphorylation of proteins within the complex sa...
ORGANISM(S): Schizosaccharomyces pombe 927 
2021-02-12 | PXD023818 | Pride
Pgc Schizosaccharomyces pombe Sax Lfq phosphoproteomics 
UnknownBenchmarking common quantification strategies for large-scale phosphoproteomics.
Not available
S-EPMC5849679 | biostudies-literature
UnknownComparative Assessment of Quantification Methods for Tumor Tissue Phosphoproteomics.
Not available
S-EPMC9366746 | biostudies-literature
UnknownPhospho-heavy-labeled-spiketide FAIMS stepped-CV DDA (pHASED) provides real-time phosphoproteomics data to aid in cancer drug selection.
Not available
S-EPMC9762002 | biostudies-literature
UnknownPhosphoproteomics and Bioinformatics Analyses Reveal Key Roles of GSK-3 and AKAP4 in Mouse Sperm Capacitation.
Not available
S-EPMC7582274 | biostudies-literature
ProteomicsDynamic phosphoproteomics of BRS3 activation reveals Hippo signaling pathway for cell migration
Bombesin receptor subtype-3 (BRS3) is an orphan G-protein coupled receptor (GPCR) that involved in a range of pathological and physiological processes, while its biological functions and underlying regulatory mechanisms remain largely unknown. Quantitative phosphoproteomics approach was employed in ...
ORGANISM(S): Homo sapiens (Human) 
2023-07-12 | PXD035666 | Pride
Phosphoproteomics Hippo signaling pathway Signaling transductions Gpcr Cell migration 
ProteomicsPhospho heavy-labeled-spiketide FAIMS stepped-CV DDA (pHASED) provides real-time phosphoproteomics data to aid in cancer drug selection
Global high-throughput phosphoproteomic profiling is increasingly being applied to cancer specimens as a means to identify the oncogenic signaling cascades responsible for promoting disease initiation and disease progression; pathways that are often invisible to genomics analysis. Hence, phosphoprot...
ORGANISM(S): Mus musculus (Mouse) 
2023-02-24 | PXD037227 | Pride
Cancer Phased Clinical phosphoproteomics Combination therapy Phosphoproteomics Resistance Atm Acute myeloid leukemia Drug targets Oncogenic signaling 
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