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ProteomicsComparison of LFQ and IPTL for protein identification and relative quantification
Mass spectrometry-based quantitative proteome profiling is most commonly performed by label-free quantification (LFQ), stable isotopic labeling with amino acids in cell culture (SILAC), and reporter-ion based isobaric labeling methods (TMT and iTRAQ). Isobaric peptide termini labeling (IPTL) was des...
ORGANISM(S): Homo sapiens (Human) 
2022-02-17 | PXD026942 | Pride
Tmt Silac Lfq Quantitative proteomics Iptl 
UnknownComparison of LFQ and IPTL for Protein Identification and Relative Quantification.
Not available
S-EPMC8433885 | biostudies-literature
UnknownLabel-Free Quantification from Direct Infusion Shotgun Proteome Analysis (DISPA-LFQ) with CsoDIAq Software.
Not available
S-EPMC9850400 | biostudies-literature
ProteomicsBenchmarking LFQ, SILAC and MS2/MS3-based TMT quantification strategies for large-scale phosphoproteomics
Comprehensive mass spectrometry (MS)-based proteomics is now feasible, but reproducible and multiplexed quantification remains challenging especially for analysis of post-translational modifications (PTMs), such as phosphorylation. Here we compared the most popular quantification techniques for phos...
ORGANISM(S): Homo sapiens (Human) Saccharomyces cerevisiae (Baker's yeast) 
2018-03-13 | PXD007145 | Pride
Phosphoproteomics Ms3 Ms2 Tmt Silac Lfq Technical Benchmark Quantification 
ProteomicsComparison of LFQ and TMT-based quantitative proteomics towards toxicology research
Here, we aimed to compare two of the most commonly used global proteomics strategies, label-free quantification (LFQ) and tandem mass tag-based quantification (TMT) to identify the most suitable tool for toxicology research questions on the systemic level. Using a biological case study of HepG2 expo...
ORGANISM(S): Homo sapiens (Human) 
2020-12-23 | PXD020248 | Pride
Toxicoproteomics Benzo[a]pyrene Tandem mass tag (tmt) Label free-quantification (lfq) Hepg2 
ProteomicsComparative assessment of quantification methods for tumor tissue phosphoproteomics
The choice of quantification method is critical for study design and comparison of cancer phosphoproteomes. We comparatively assessed label-free quantification (LFQ), spike-in-SILAC (stable isotope labeling of amino acids in cell culture) and tandem mass tag (TMT) labeling techniques for quantitativ...
ORGANISM(S): Homo sapiens (Human) 
2022-08-09 | PXD030450 | Pride
Phosphoproteomics Tissue Ovarian cancer Quantification 
UnknownLFQ-Based Peptide and Protein Intensity Differential Expression Analysis.
Not available
S-EPMC10243145 | biostudies-literature
ProteomicsLFQ Burkholderia Cenocepacia mutant comparsion
Comparsion of multiple BC k56-2 muants to assess protoeme chnages. seven strain comparsion between WT vs delta pglL vs delta 1086 vs delta 2974 vs delta CepI vs delta CepR vs delta pglL complemented AmrAB::S7-pglL-his. LFQ based quantification
ORGANISM(S): Burkholderia cenocepacia K56-2Valvano 
2019-11-01 | PXD014581 | Pride
Glycosylation mutant Lfq Burkholderia Quorum sensing 
ProteomicsComparison of DIA and TMT based protein quantification in complex background
Label free quantification (LFQ) and isobaric labelling quantification (ILQ) are among the most popular protein quantification workflows in discovery proteomics. Here, we compared the TMT 10-plex workflow to label free single shot data-independent acquisition (DIA) method on a controlled sample set. ...
ORGANISM(S): Mus Musculus (ncbitaxon:10090) 
2020-01-29 | MSV000084859 | MassIVE
ProteomicsLabel-free quantification (LFQ) analysis of Sphingopyxis granuli TFA wild-type and ΔsuhB strains during exponential and stationary growth phases
A comparative LFQ-based proteomic analysis was performed to assess differences in protein abundance between Sphingopyxis granuli TFA WT and the small RNA mutant ΔsuhB strains during exponential and stationary growth in minimal medium supplemented with 40 mM β-hydroxybutyrate (BHB)
ORGANISM(S): Sphingopyxis granuli Bacteria 
2026-05-18 | PXD073771 | Pride
Proteomics Suhb Small rna Regulon 
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