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ProteomicsAn Undesirable Side Effect of EDC-catalyzed Carboxyl Labeling Approach
Carbodiimide catalyzed carboxyl and amine conjugation (amidation) has been widely used to protect carboxyl groups. N-(3-dimethylaminopropyl)-N’-ethylcarbodiimide (EDC) is the most commonly used carbodiimide reagent in protein chemis-try due to its high efficiency and high solubility in aqueous media...
ORGANISM(S): Homo sapiens (Human) 
2020-12-10 | PXD020042 | Pride
Tyrosine Carboxyl protection C-tails Edc 
ProteomicsLys-C/Arg-C, a New Enzyme Combination for Next-Generation Proteomics
Today, the most widely used bottom-up proteomics studies necessitate a proteolysis step prior to MS analysis. Trypsin is often the best protease in choice due to its high specificity and MS-favored proteolytic products. Trypsin has been challenged for its low cleavage efficiency at Lys-X bonds, and ...
ORGANISM(S): Homo sapiens (Human) 
2018-07-24 | PXD009797 | Pride
Lys-c / arg-c / trypsin / dia / mass spectrometry 
ProteomicsBasic Strong Cation Exchange Chromatography, BaSCX, a Highly Efficient Approach for C-Terminomic Studies Using LysargiNase Digestion
Decoding protein C-termini is a challenging task in protein chemistry using conventional chemical/enzymatic approaches. With the rapid development in modern mass spectrometer, many advanced mass spectrometry (MS) based protein C-termini analysis approaches have been established. Although great progr...
ORGANISM(S): Homo sapiens (Human) Mus musculus (Mouse) Caenorhabditis elegans 
2020-02-28 | PXD016317 | Pride
C-terminomics / lysarginase / scx / bascx / mass spectrometry 
UnknownUltralow-temperature-driven water-based sorption refrigeration enabled by low-cost zeolite-like porous aluminophosphate.
Not available
S-EPMC8752593 | biostudies-literature
UnknownIodide-mediated intermediate regulation strategy enables high-capacity and ultra-stable zinc-iodine batteries.
Not available
S-EPMC12184782 | biostudies-literature
ProteomicsCombination of multi-enzyme digestions and C-TAILS approach for deep screening of C-terminome
Protein C-termini study is still a challenging task and far behind its counterpart N-termini study. The identification of C-termini is often hampered by low ionization response in MS and fewer efficient enrichment methods existing. We previously optimized the C-TAILS (C-terminal amine-based isotope ...
ORGANISM(S): Escherichia coli 
2017-11-24 | PXD004275 | Pride
Protein c-termini C-tails Multi-enzyme Scii 
ProteomicsSystematic optimization of C-TAILS approach for deep screening of C-terminomics
It is well known that protein C-termini play important roles in various biological processes, and thus the precise characterization of C-termini is essential for fully elucidating protein structure and understanding protein functions. Although many efforts have been made in the fields during the lat...
ORGANISM(S): Escherichia coli 
2015-09-15 | PXD002409 | Pride
Protein c-termini Acetylation Scii 
UnknownConvergent transcriptomic and genomic evidence supporting a dysregulation of CXCL16 and CCL5 in Alzheimer's disease.
Not available
S-EPMC9863145 | biostudies-literature
ProteomicsA Tribrid Hyperplexing approach for Large-scale Quantitative Proteomic Analysis
Isobaric labeling has emerged as an indispensable quantitative proteomic approach for its unprecedented multiplexing capacity in a single analysis. Currently, different hyperplexing approaches have been developed to meet the need for increasing sample numbers in large-scale cohort analysis. In this ...
ORGANISM(S): Homo sapiens (Human) Escherichia coli 
2023-03-17 | PXD037498 | Pride
Tmtpro Ibt Tmt Hyperplexing Quantitative proteomics 
ProteomicsEnzyme and Chemical Assisted N-Terminal Blocked Peptides Analysis, ENCHANT, as a Selective Proteomics Approach Complementary to Conventional Shotgun Approach
Shotgun (bottom-up) approach has been widely applied in large-scale proteomics studies. The inherent shortages of shotgun approach lie in that the generated peptides often overwhelm the analytical capacity of current LC-MS/MS systems and that abundant proteins often hamper the identification of prot...
ORGANISM(S): Homo sapiens (Human) 
2017-11-30 | PXD007863 | Pride
Dia Single-charge ion inclusion N-terminal blocked peptides Cnbr-activated sepharose 4b 
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