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Identification of the host factors trigger ORF9b degradation
ORGANISM(S): Homo Sapiens 
2024-02-23 | PXD050055 |
EV71-VP2-MYC was extransfected into 293T cells, and 36 hours later, the cells were treated with MG132 for 12 hours. The cells were harvested, and the cell lysates were incubated with protein-G with MYC antibody overnight. The interacting proteins were analyzed by mass spectrometry.
ORGANISM(S): Homo Sapiens 
2025-01-07 | PXD059489 |
HEK293T were transfected with SARS-CoV-2-NSP16-HA for 36 h, then treated with MG132 or DMSO for 12 h prior to harvest. Co-IP assay were performed with HA beads (Roche, catalog no. 11867423001), the elution was analyzed by mass spectrum. Mass spectrum analysis were performed by the national center fo...
ORGANISM(S): Homo Sapiens 
To identify potential host factors promoting HIV-1 control and non-progressive infection, we employed mass spectrometry-based proteomic profiling to analyze three LTNPs and two PRs,in order to identify proteins expressed at higher levels in LTNPs.
ORGANISM(S): Homo Sapiens 
The whole blood samples were obtained from 3 LTNPs and 2 RPs HIV-1 infected patients, and the CD4+ T cells were isolated using anti-CD4-specific antibody-coated microbeads (130-045-101, Miltenyi Biotec, Germany) according to the manufacturer's instructions. Then the proteins extracted from the CD4+ ...
ORGANISM(S): Homo Sapiens 
HEK293T were transfected with SARS-CoV-2-E-HA for 36 h, then treated with MG132 or DMSO for 12 h prior to harvest. Co-IP assay were performed with HA beads (Roche, catalog no. 11867423001), the elution was analyzed by mass spectrum. Mass spectrum analysis were performed by the national center for pr...
ORGANISM(S): Homo Sapiens 
2023-01-01 | PXD039140 |
HEK293T were transfected with RSV-SH-HA, GBP5-HA or VR1012(empty vector) respectively for 48 h and then harvested. Co-IP assay were performed with HA beads (Roche, catalog no. 11867423001), the elution was analyzed by mass spectrum. Mass spectrum analysis were performed by the national center for pr...
ORGANISM(S): Homo Sapiens 
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