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Isolated murine kidney glomeruli were lysed in buffer containing 8M urea, and proteins were reduced and alkylated. Proteins were digested with trypsin. After reverse-phase chomatrography, peptides were fractionated using strong cation exchange chromatography. Phosphopeptides were enriched using IMAC...
ORGANISM(S): Mus musculus (Mouse) 
2016-05-09 | PXD000266 | Pride
Current strategies used to quantitatively describe the biological diversity of lipids by mass spectrometry are often limited in assessing the exact structural variability of individual molecular species in detail. A major challenge is represented by the extensive isobaric overlap present among lipid...
2021-01-11 | MTBLS636 | MetaboLights
Primary effusion lymphoma (PEL) is caused by Kaposi's sarcoma-associated herpesvirus (KSHV) and frequently also harbors Epstein-Barr virus (EBV). The expression of KSHV- and, often, EBV-encoded microRNAs (miRNAs) in PELs suggests a role for these miRNAs in viral latency and lymphomagenesis. Here we ...
ORGANISM(S): Homo sapiens 
RBM4 is a RNA-binding protein (RBP) able to modulate splicing by promoting exon inclusion and shares an import pathway with other splicing factors in the nucleus. In earlier studies we found that RBM4 interacts and colocalizes with WT1, which has been implicated in 10M-bM-^@M-^S15% of WilmsM-bM-^@M-...
ORGANISM(S): Homo sapiens 
We analyzed microdissected cortical collecting ducts of pendrin knockouts using a sensitive targeted proteomics approach proteomics approach.
ORGANISM(S): Mus musculus (Mouse) 
2022-04-04 | PXD018791 | Pride
We used a targeted proteomics assay to survey proximal tubule function.
ORGANISM(S): Mus musculus (Mouse) 
2018-06-28 | PXD006896 | Pride
Human ZRANB2 mRNA is alternatively spliced to give two variants with different 3M-bM-^@M-^Y ends. Both isoforms are present in the nucleus of human cells. ZRANB2 binds to mRNA, as well as the essential splicing factors U170K and U2AF35, and the novel splicing component SFRS17A (formerly known as XE7...
ORGANISM(S): Homo sapiens 
Phytochrome Interacting Factor 5 plays an important role in adaptive responses of plants to shaded environment collectively called shade avoidance syndrome. PIF 5 belongs to the bHLH transcription factor family and regulated gene expression in a low R/FR dependent fashion. In this experiment we inve...
ORGANISM(S): Arabidopsis thaliana 
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