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Transcription steps are marked by different modifications of the C-terminal domain of RNA polymerase II (RNAPII). Phosphorylation of Ser5 and Ser7 by cyclin-dependent kinase 7 (CDK7) as part of TFIIH marks initiation, whereas phosphorylation of Ser2 by CDK9 marks elongation. These processes are thou...
ORGANISM(S): Mus musculus 
CALIPERS: Cell cycle-aware live imaging for phenotyping experiments and regeneration studies
Morphodynamic domains enable integration of live imaging morphometrics with spatial transcriptomics
Assembly of infectious influenza A viruses (IAV) is a complex process involving transport from the nucleus to the plasma membrane. Rab11A containing recycling endosomes have been identified as a platform for intracellular transport of viral RNA (vRNA). Using high spatiotemporal resolution light-shee...
ORGANISM(S): Homo sapiens (Human) Influenza A virus (A/California/07/2009(H1N1)) 
2020-01-14 | PXD016331 | Pride
Quantitative live-cell imaging of secretion activity reveals dynamic immune responses
Next-generation breast organoids capture human organogenesis with high-resolution live imaging
To allow the study of unsaturated free fatty acids in live cells, we here report the use of sterculic acid, a 1,2-cyclopropene containing oleic acid analogue, as a bioorthogonal probe. We here show that this lipid can be readily taken up by dendritic cells without toxic side-effects, and that it can...
ORGANISM(S): Mus musculus (Mouse) 
2023-04-28 | PXD033831 | Pride
We introduce CALIPERS, a Fluorescence Ubiquitin Cell Cycle Indicator enabling cell cycle-aware phenotyping based on multiplexed live-cell imaging. We validate CALIPERS in three- and four-color reporter lines (HaCaT) and human induced pluripotent stem cells (WTC-11), co-expressing genetically encoded...
ORGANISM(S): Homo sapiens 
2026-06-25 | GSE281528 | GEO
A Split Luciferase Biosensing Platform for Detection and Imaging of Chromatin Loops in Individual Live Cells
Decoding the interplay between m6A modification and stress granule stability by live-cell imaging
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